Oxytocin antagonist does not disrupt rabbit maternal behavior despite binding to brain oxytocin receptors

Abstract

We explored a possible role of oxytocin (OXT) for the onset and maintenance of rabbit maternal behavior by: (a) confirming that a selective oxytocin receptor antagonist (OTA) widely used in rodents selectively binds to OXT receptors (OXTR) in the rabbit brain and (b) determining the effect of daily intracerebroventricular (icv) injections of OTA to primiparous and multiparous does from gestation day 29 to lactation day 3. OTA efficiently displaced the high affinity, selective oxytocin receptor (OXTR) radioligand, 125I‐labeled ornithine vasotocin analog (125I‐OVTA), but was much less effective at displacing the selective V1a vasopressin receptor radioligand, 125I‐labeled linear vasopressin, thus showing high affinity and selectivity of OTA for rabbit OXTR as in rodents. Further, ICV OTA injections did not modify nest‐building, latency to enter the nest box, time spent nursing or the amount of milk produced, relative to vehicle‐injected does. The percentage of mothers suckling the litter was also similar between both groups, regardless of parity. Together, our results do not support a role of OXT for the initiation or maintenance of rabbit maternal behavior. Future studies are warranted to determine if OXT participates in fine‐tuning additional aspects of the maternal ethogram, for example, circadian periodicity of nursing and nest defense.

The Role of Oxytocin in Domestic Animal’s Maternal Care: Parturition, Bonding, and Lactation

“…The investigations of OXT in different anatomical regions and compounds are another research field. Recent studies in rabbits have shown that OXT antagonist administration does not impair maternal behavior [ 175 ]; however, studies by Keverne and Kendrick [ 99 ] have reported that OXT’s effect on maternal neuroendocrine regulation is affected by the administration of opioid receptor blockers, limiting OXT release, a relevant issue when using analgesic treatment or other therapeutic protocols during parturition [ 27 ]. Moreover, nursing and maternal traits are highly motivated by parity, species, experience, and intrapartum events such as dystocia [ 87 ].…”