Oxytocin and tumor necrosis factor α stimulate expression of prostaglandin E2 synthase and secretion of prostaglandin E2 by luminal epithelial cells of the porcine endometrium during early pregnancy

Wacławik, Agnieszka; Blitek, Agnieszka; Ziȩcik, Adam J.

doi:10.1530/rep-10-0092

Cited by 34 publications

(33 citation statements)

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“…PGE 2 , after reaching the ovaries, may act through luteal PGE 2 receptors (PTGER), PTGER2 and PTGER4 (Waclawik et al 2010). Other subtypes of PTGER have not been studied in the CL in this species yet.…”

Section: Role Of Pgs During Pregnancy Establishmentmentioning

confidence: 99%

“…The source of TNF may be macrophages, which are present in the endometrium during the estrous cycle and early pregnancy, as well as endometrial cells and conceptus (Hunt et al 1996). Recent studies indicate that TNF stimulates PGE 2 synthesis and secretion through upregulation of PTGS2 mRNA, as well as mPGES-1 mRNA and protein content in porcine LE cells collected from gilts on days 11-12 after estrus (Waclawik et al 2010).…”

Section: Nk Cellsmentioning

confidence: 99%

“…The role of oxytocin (OXT) in controlling PGF 2a secretion is not as well defined in pigs as in ruminants (Mirando et al 1995, Waclawik et al 2010. In ewes, luteal OXT acts through endometrial OXT receptors to generate a PGF 2a pulse which in turn stimulates further secretion of OXT as well as inducing functional regression of the CL through inhibiting progesterone synthesis (reviewed in Jenkin (1992)).…”

Section: Introductionmentioning

confidence: 99%

“…infusion of OXT decreases plasma concentrations of PGF 2a metabolite, PGFM, on day 16 after estrus (Sample et al 2004). In addition, OXT stimulates synthesis and secretion of PGE 2 (PG which has the opposite action to PGF 2a on the CL) by endometrial luminal epithelial (LE) cells only during early pregnancy but not on the corresponding days of the estrous cycle (Waclawik et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Novel insights into the mechanisms of pregnancy establishment: regulation of prostaglandin synthesis and signaling in the pig

Wacławik

2011

Reproduction

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Ovarian progesterone induces essential changes leading to a temporary state of uterine receptivity for conceptus implantation. Estrogens secreted by the porcine conceptus on days 11 and 12 of pregnancy provide the initial signal for maternal recognition of pregnancy and maintenance of a functional corpus luteum (CL) for continued production of progesterone. As prostaglandins F 2a (PGF 2a ) and E 2 (PGE 2 ) exert opposing actions on the CL, a tight control over their synthesis and secretion is critical either for the initiation of luteolysis or maintenance of pregnancy. One of the supportive mechanisms by which conceptus inhibits luteolysis is changing PG synthesis in favor of luteoprotective PGE 2 . Conceptus PGE 2 could be amplified by PGE 2 feedback loop in the endometrium. In pigs, as in other species, implantation and establishment of pregnancy is associated with upregulation of expression of proinflammatory factors, which include cytokines, growth factors, and lipid mediators. The conceptus produces inflammatory mediators: interferon g and interferon d, interleukins IL1B and IL6, and PGs, which probably activate inflammatory pathways in the endometrium. The endometrium responds to these embryonic signals by enhancing further progesterone-induced uterine receptivity. Understanding the mechanisms of pregnancy establishment is required for translational research to increase reproductive efficiencies and fertility in humans and animals.

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Section: Role Of Pgs During Pregnancy Establishmentmentioning

confidence: 99%

Section: Nk Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel insights into the mechanisms of pregnancy establishment: regulation of prostaglandin synthesis and signaling in the pig

Wacławik

2011

Reproduction

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“…Oestrogens and ovarian progesterone make up the specific hormonal microenvironment (Bazer and Johnson, 2014). The first oestrogens peak (days 10 to 12 of gestation) stimulates prostaglandin E2 (PGE2) synthesis and increases the PGE2/ prostaglandin F2α (PGF2α) ratio (Waclawik et al, 2009), boosts endometrial production of fibroblast growth factor 7 (FGF7), transforming growth factor beta (TGFβ), interleukin-1β, tumour necrosis factor alpha (TNFα), interferon delta (IFND) and interferon gamma (IFNG) (Harney and Bazer, 1989;Ka et al, 2001;Ross et al, 2003;White et al, 2009;Waclawik et al, 2010). The discussed growth factors and cytokines directly influence various genes whose products deliver luteoprotective effects and support conceptus development (Bazer and Johnson, 2014).…”

Section: Discussionmentioning

confidence: 99%

Expression of the orexin system in the porcine uterus, conceptus and trophoblast during early pregnancy

Smolińska

Kieżun

Szeszko

et al. 2015

Animal

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Orexin A and B are hypothalamic peptides derived from the prepro-orexin (PPO) precursor. Orexins stimulate food intake and arousal. Those peptides bind and activate two G protein-coupled receptors: orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). Numerous authors have suggested that orexins play an important role in the regulation of the reproductive functions. The objective of the present study was to analyse the presence of and changes in the gene and protein expression pattern of the orexin system in the porcine uterus, conceptus and trophoblast (chorioallantois) during early pregnancy. In the endometrium, the highest PPO and OX1R gene expression was detected on days 15 to 16 of gestation. The OX2R mRNA content in the endometrium was higher on days 10 to 11 and 15 to 16 than on days 12 to 13 and 27 to 28. In the trophoblasts, PPO gene expression was higher on days 30 to 32 than on days 27 to 28. The highest PPO protein content in the endometrium was noted on days 12 to 13. The highest OX1R protein content in the endometrium was detected on days 10 to 11, whereas OX2R protein on days 15 to 16. In the trophoblasts, PPO and OX1R protein levels were more pronounced on days 27 to 28 than on days 30 to 32, but OX2R expression was higher on days 30 to 32. The expression of PPO, OX1R and OX2R was different in the conceptuses and trophoblasts during early pregnancy. Local orexin production and the presence of the specific orexin receptors suggest that the orexin system may participate in the control of porcine reproductive functions by exerting endocrine and auto/paracrine effects on the uterus, conceptuses and trophoblasts during early pregnancy. This study provides the first evidence for the presence of orexins and their receptors in the uteri, conceptuses and trophoblasts in pigs during early pregnancy. The local orexin system is dependent on the stage of pregnancy.Keywords: orexin A and B, orexin receptors 1 and 2, uterus, pregnancy, pig ImplicationsThere is a growing body of evidence to implicate the existence of a common endocrine system that controls metabolism and the reproductive system. Orexin A and orexin B, the hormones that control energy homoeostasis, may be a part of the above system. This paper provides the pioneering information about the presence of orexins and orexin receptors in the porcine uterus, conceptus and trophoblast during early pregnancy, the crucial period for reproductive success. The expression levels of orexins and their receptors were found to be correlated with the stages of early pregnancy. A sound knowledge of mechanisms that control energy homoeostasis and reproduction is needed in animal breeding practice.

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Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

Wacławik

Kaczmarek

Blitek

et al. 2017

Molecular Reproduction Devel

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102

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Porcine conceptuses secrete pregnancy-recognition signals (estrogens, including estradiol-17β) that inhibit luteolysis, thereby prolonging progesterone production by corpora lutea. The supportive mechanism by which the conceptus also inhibits luteolysis is by shifting endometrial prostaglandin (PG) synthesis to luteoprotective PGE2.Progesterone stimulates endometrial production of factors that are essential for conceptus development. Priming the uterus by progesterone and loss of progesterone receptors from the uterine epithelium by Day 10-12 after estrus are key for achieving endometrial receptivity for implantation. Conceptus implantation involves a series of events, many resembling the inflammatory reaction, that are greatly influenced by cytokines, growth factors, and prostaglandins. We herein present a novel, dual role for PGF2α in corpora lutea that depends on the acquisition of luteolytic sensitivity, based on the knowledge that PGF2α triggers pathways involved in luteolysis during the estrous cycle or/and may have an alternative function in maintaining progesterone synthesis during pregnancy. We also point out a new role for PGF2α that, together with PGE2, can act as embryonic signal mediators. PGF2α, which until recently was considered undesirable for promoting pregnancy, is now known to stimulate conceptus-maternal interactions and angiogenesis in the endometrium. This function is in line with other important prostaglandin functions, such as stimulating adhesion of trophoblasts (PGE2, PGI2) as well as endometrial vascular functions and trophoblast cell proliferation (PGI2).

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Oxytocin and tumor necrosis factor α stimulate expression of prostaglandin E2 synthase and secretion of prostaglandin E2 by luminal epithelial cells of the porcine endometrium during early pregnancy

Cited by 34 publications

References 53 publications

Novel insights into the mechanisms of pregnancy establishment: regulation of prostaglandin synthesis and signaling in the pig

Novel insights into the mechanisms of pregnancy establishment: regulation of prostaglandin synthesis and signaling in the pig

Expression of the orexin system in the porcine uterus, conceptus and trophoblast during early pregnancy

Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

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