Oxytocin and plasma containing leukocytes or a combination of both as treatment of postbreeding endometritis in the horse

Mattos, Rodrigo Costa; Gregory, Ricardo Macedo; Meirelles, Luciana Silva; Malschitzky, Eduardo; Castilho, L F F; Neves, Adriana Pires; Mattos, Ana Luiza Gelpi; Vieira, Magda Jöchins; Keller, Andrea; Hott, A.K.

doi:10.21836/pem19990618

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…Data presented in this work showed that the pregnancy rate of maiden mares bred in the 1 st estrus cycle during the breeding season was significantly lower (63.7%) than that of the mares bred in other estrus cycles (80.3%), results similar to those observed previously by Mattos et al (1997 and1999). However, no significant difference (p>0.05) regarding the EL rate was observed between the first and other estrus cycles, being the EL rates in agreement with those found by Ginther (1992).…”

Section: Discussionsupporting

confidence: 91%

Pregnancy and embryo loss rates in non-lactating mares bred in the first or in other estrus cycles during the breeding season

Malschitzky¹,

Schilela²,

Mattos³

et al. 2003

PHK

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The aim of the present study was to compare pregnancy and embryo loss rates in non-lactating mares bred either in the first, or in other estrus cycles during the breeding season. A total of 324 non-lactating mares, 179 of them barren and 145 maiden, were allocated in a stallion management center in Southern Brazil. All the mares were submitted to an artificial photoperiod of 15 hours light/9 hours darkness, either from May 15th on, or later on, from the moment they entered the management center. All the mares have been examined twice a week, from July 15th to July 31st, in order to determine the estrus phase, being classified, according to the findings, into two groups: group 1 = 1st estrus-mares showing reduced uterine tonus, small sized ovaries or ovaries with irregular follicular activity, lacking evidence of preovulatory follicle and, mainly, of corpus luteum (CL) at ultrasound; group 2 = ∆ 2 nd estrus-mares presenting CL at examination. The last ones received Prostaglandin on the 5th day after CL identification, or later on, for induction of a new estrus cycle. From August 1st on, all mares in estrus have been examined at maximal intervals of 48 hours, in order to evaluate follicular growth, grade of uterine edema and presence of intra-uterine fluid (IUF). Mares have been bred when a preovulatory follicle and low grade of uterine edema were found. A new examination was performed 36 to 48 hours after breeding to confirm ovulation and to detect IUF. Mares showing IUF were treated by means of uterine flushes and antibiotic infusion and those showing IUF for more than three consecutive days were excluded from the experiment. Barren and maiden mares bred in the 1st estrus cycle showed significant lower (p<0,026) pregnancy rates in days 12 and 42 in comparison to mares bred during subsequent cycles. Barren mares bred during the 1st estrus cycle showed higher embryo loss rate (p= 0.048) than barren mares bred during other cycles (20.4% vs. 8.0%). Embryo loss rate was not significantly different in maiden mares bred in 1st or in other estrus (9.1% vs. 4.9%). Grade of uterine edema and presence of IUF during estrus did not differ in barren and maiden mares bred either in the 1st, or in other cycles. However, barren mares bred during the 2nd and other cycles showed higher incidence of IUF (p=0.040) 36 to 48 hours after breeding than those bred in the 1st estrus cycle (51.7% vs.27.5%). Presence of IUF was not significantly different (p=0.680) between maiden mares bred during the 1st or other cycles (18,2% vs. 27.0%). It was concluded that: (a) during the breeding season the fertility of non-lactating mares is lower in the 1st than in other estrus cycles; (b) that a higher embryo loss rate may be expected in barren mares bred in the 1st than in other estrus cycles of the breeding season and (c) that the lower fertility rate observed during the 1st estrus cycle is not related to the incidence of post-breeding endometritis.

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Section: Discussionsupporting

confidence: 91%

Pregnancy and embryo loss rates in non-lactating mares bred in the first or in other estrus cycles during the breeding season

Malschitzky¹,

Schilela²,

Mattos³

et al. 2003

PHK

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“…The intrauterine infusion of freshly isolated autologous PMN as a treatment of equine endometritis is routinely applied in several Stud-farms in Brazil. Mattos et al (1999) could show that the early pregnancy rate per cycle was significantly higher in barren mares treated with plasma containing neutrophils than in mares treated with oxytocin and in control mares. However, this procedure has some practical limitations, especially if many mares have to be treated at the same time.…”

Section: Discussionmentioning

confidence: 92%

“…The use of peripheral blood neutrophils together with autologous plasma, as a post-breeding treatment, resulted in improved fertility in barren mares. In experimentally infected mares, this treatment helped to eliminate bacteria in less time compared with other treatments (Mattos et al, 1999).…”

Section: Introductionmentioning

confidence: 94%

Isolation and Cryopreservation of Functionally Competent Equine Leucocytes

Zerbe¹,

Castilho

Engelke

et al. 2003

Journal of Veterinary Medicine Series A

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Sufficient numbers of functionally competent polymorphonuclear neutrophil granulocytes (PMN) seem to be of major importance during the course of equine endometritis. In this study, we wanted to establish a method for cryopreservation of functionally competent neutrophils for an intended local endometritis therapy in mares. The separation of leucocytes by hypotonic lysis of whole blood from clinically healthy mares was superior to the separation by dextrose sedimentation. After suspension of the cells in the cryoprotective solution [equine plasma with 5% (v/v) dimethyl sulphoxide (DMSO)], the leucocytes were frozen in liquid nitrogen. A temperature gradient with low cooling velocity (1 degree C/min between 4 and -70 degrees C) resulted in highest numbers of viable cells after thawing. Thawed PMN had a high phagocytic capacity for opsonized streptococci. Their ability to generate reactive oxygen species (ROS) after stimulation with a phorbol ester was even higher than that of freshly isolated PMN and was preserved up to 6 h after thawing. The results of this study indicate that cryopreservation of PMN may provide viable and functionally competent neutrophils for therapeutic use in mares susceptible to endometritis.

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Use of leukocytes as treatment for endometritis in mares experimentally infected with Streptococcus equi subsp. zooepidemicus

Neves

Keller

Trein

et al. 2007

Animal Reproduction Science

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Oxytocin and plasma containing leukocytes or a combination of both as treatment of postbreeding endometritis in the horse

Cited by 4 publications

References 7 publications

Pregnancy and embryo loss rates in non-lactating mares bred in the first or in other estrus cycles during the breeding season

Pregnancy and embryo loss rates in non-lactating mares bred in the first or in other estrus cycles during the breeding season

Isolation and Cryopreservation of Functionally Competent Equine Leucocytes

Use of leukocytes as treatment for endometritis in mares experimentally infected with Streptococcus equi subsp. zooepidemicus

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