Oxytalan Fibres in the Periodontal Ligament of Equine Molar Cheek Teeth

Abstract

The distribution and arrangement of oxytalan fibres were examined in periodontal specimens of cheek teeth from seven horses. Oxidation prior to aldehyde fuchsin exposition permitted a selective staining of the oxytalan fibres, which are a distinct component of the elastic fibre system. On three horizontal levels of the periodontium--(a) subgingival, (b) middle third and (c) apical--two oxytalan fibre groups were shown histologically: 'blood vessel-related' and 'independent' oxytalan fibres. In levels a and b, both groups were arranged in a typical occluso-apical alignment along the reserve crown. Single oxytalan fibres deviated from their general course in order to attach to the cementum. In these cemental entheses the oxytalan fibres ran parallel to collagen fibre bundles. The interpretation of such morphological features emphasized the oxytalan fibres' capacity to improve the stability of periodontal blood vessel walls during masticatory movements. Level c, especially in regions next to the persisting epithelial root sheath, is the site of oxytalan fibre generation. This is a prerequisite for the facilitation of periodontal regeneration and reorganization during dental growth and eruption.

ADAMTSL6β Protein Rescues Fibrillin-1 Microfibril Disorder in a Marfan Syndrome Mouse Model through the Promotion of Fibrillin-1 Assembly

“…An anti-Adamtsl6 polyclonal antibody was labeled with Alexa 488 by using the Zenon antibody labeling kit according to the manufacturer's instructions (Invitrogen) for double immunostaining with an anti-fibrillin-1 polyclonal antibody. For visualization of oxytalan fibers, sections were oxidized for 15 min in 10% Oxone (Merck) and subsequently stained with aldehyde fuchsin as described previously (20). Fluorescence images were sequentially collected using a confocal microscope featuring 403-, 488-, and 543-nm laser lines (LSM510; Carl Zeiss MicroImaging, Jena, Germany).…”

Trends in Periodontal Regeneration Therapy: Potential Therapeutic Strategy of Extracellular Matrix Administration for Periodontal Ligament Regeneration

“…ADAMTSL6β and fibrillin-1-positive microfibrils (green yellow) was markedly increased in the aorta and skin of TSL6β TG mice compared with WT mice. Bar ¼ 50 μm Image from published paper [39] Since oxytalan fiber, a principal elastic fiber system of PDL is composed of fibrillin-1 microfibrils and does not contain significant amounts of elastin [44,45], this composition suggests that PDL will have an increased susceptibility to breakdown in MFS compared with other elastic tissues composed of both elastin and fibrillin-1 [46]. We demonstrated that ADAMSL6β is highly expressed in DF during PDL forming stage.…”

Tooth and Tooth-Supporting Structures

“…Oxytalan fi bers are composed of 10-12 nm wide microfi brils without any amorphous elastin substance. They are oriented in an occluso-apical direction and topographically related to the periodontal vascular system [ 49 ]. Elaunin fi bers may also be found in association with fi ber bundles in the gingival ligament.…”