Oxyresveratrol induces apoptosis and inhibits cell viability via inhibition of the STAT3 signaling pathway in Saos‑2 cells

Abstract

oxyresveratrol (oreS) is a natural phenolic compound with multiple biological functions including antioxidation, anti-inflammation and neuroprotection; however, the inhibitory effect of oreS on osteosarcoma remains largely unknown. The present study aimed to determine the effects of oreS on osteosarcoma cell Saos-2. cell counting Kit-8 assay was performed to detect Soas-2 cell viability. annexin-FiTc/Pi staining and Jc-1 staining were used to measure cell apoptosis and the change of mitochondrial membrane potential. in addition, western blotting was conducted to determine the expression levels of apoptotic proteins and the phosphorylation of STaT3. it was found that oreS inhibited cell viability and induced apoptosis of osteosarcoma Saos-2 cells in a concentration-dependent manner. in addition, oreS increased the expression levels of apoptotic proteases caspase-9 and caspase-3 and reduced mitochondrial membrane potential. in response to oreS treatment, the expression levels of pro-apoptotic proteins, Bad and Bax, were enhanced, whereas those of anti-apoptotic proteins, Bcl-2 and Bcl-xl, were reduced. in addition, the phosphorylation of STaT3 was attenuated in Saos-2 cells after treatment with oreS. inhibition of cell viability and apoptosis induction by oreS were rescued by enhancement of STaT3 activation upon treatment with il-6. collectively, the present study indicated that oreS induced apoptosis and inhibited cell viability, which may be associated with the inhibition of STAT3 activation; thus, ORES represents a promising agent for treating osteosarcoma.

Oxyresveratrol: Sources, Productions, Biological Activities, Pharmacokinetics, and Delivery Systems

“…In osteosarcoma cell Saos-2 cells, ORV inhibited cell viability and induced apoptosis by suppressing the activation of STAT3 (signal transducer and activator of transcription 3) [ 213 ]. ORV also induced apoptosis of human bladder cancer T24 cells in a dose- and time-dependent manner [ 214 ].…”

Oxyresveratrol Ameliorates Dextran Sulfate Sodium-Induced Colitis in Rats by Suppressing Inflammation

“…Thus, the Bax/Bcl-2 ratio is often used as an index of apoptosis within tissue. OXY enhances pro-apoptotic proteins and reduces anti-apoptotic proteins in osteosarcoma cells, which induces apoptosis in the cancer cells through inhibition of the STAT3 signaling pathway [ 32 ]. However, in this study, OXY decreased Bax/Bcl-2 ratio, which means that OXY protects intestinal goblet cells from apoptosis.…”

Traditional Medicinal Plants as a Source of Inspiration for Osteosarcoma Therapy

“…Also, oxyresveratrol revealed promising results in osteosarcoma treatment. The natural product inhibited Saos–2 cell viability and induced apoptosis by reducing the phosphorylation level of STAT3 [ 116 ]. However, further studies of the underlying mechanisms of phillygenin and oxyresveratrol are still needed to elucidate the applicability of these novel compounds for osteosarcoma therapy.…”