Oxygenated Secondary Metabolites from Endophytic Fungi Isolated From Ocimum sanctum

Abstract

Ocimum sanctum Linn (Tulsi), an important medicinal plant of Bangladesh is used for antimicrobial, antiinflammatory and antiulcer activities. Eleven endophytic fungi were isolated from O. sanctum. The fungal strain, labeled as OS-005, was cultivated at large scale on the potato dextrose agar semi solid medium and was extracted with ethyl acetate. Repeated column chromatography of the parent extract by normal phase silica gel and one of its fraction by Sephadex LH-20 afforded linoleic acid, R (-)-glycerol monolinoleate, 9,10,11-trihydroxy-(12Z)-12-octadecenoic acid, ergosterol, ergosterol peroxide, 1,8-O-dimethylaverantin, coriloxin, and a new natural product named sanctumol. The structures of these compounds were elucidated unequivocally by UV, IR, MS and a series of 1D & 2D NMR analyses.

Pharmacological potential of fungal endophytes associated with the genus Ocimum L.

“…Studies by Mohammad et al, (2015) on the crude extract of endophytes isolated from O.tenuiflorum, elucidated the structure of linoleic acid, R (-)-glycerol monolinoleate, 9, 10, 11,trihydroxy-(12Z)-12-octadecenoic acid, ergosterol, ergosterol peroxide, 1,8-Odimethylaverantin, coriloxin, and a new natural product named as sactumol. Studies of Gangadevi & Muthumary, (2007) showed that leaf endophyte Phyllosticta (sp.6) is a potential producer of taxol in artificial culture media.…”