Oxygen Transport Through a Model Lung Surfactant Surface Layer: Influence of the Film Compression on the Kinetics

Ladanyi, Erna; Ahuja, Ramesh C.; Möbius, Dietmar; Stalder, Karlheinz

doi:10.1007/978-1-4615-3428-0_37

Cited by 3 publications

(5 citation statements)

References 3 publications

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“…Thus, increasing the hydrophobic chain length of the coating lipid provides a valuable means of increasing the stability of therapeutic microbubbles. The result that the longer chain lipids impose a significant barrier to air permeation is biologically relevant, most notably for studies involving gas exchange in the lungs 3 Shell Resistance to Air Permeation carbon chain length of main lipid componentnumber of bubblesresistance (s/cm) 12 5 0 14 16 49 ± 21 16 23 51 ± 27 17 12 49 ± 21 18 19 86 ± 56 19 9 153 ± 75 20 22 166 ± 55 21 6 225 ± 52 22 11 315 ± 72 24 23 370 ± 149 …”

Section: Resultsmentioning

confidence: 99%

“…The result that the longer chain lipids impose a significant barrier to air permeation is biologically relevant, most notably for studies involving gas exchange in the lungs. 44 Two general mechanisms, one based on the continuum approximation and the other on statistical mechanics, have been proposed to describe the permeation of gas molecules through monolayer films. The size of the permeating gas molecule is approximately an order of magnitude smaller than the length scale over which permeation is taking place (i.e., the thickness of the shell).…”

Section: Resultsmentioning

confidence: 99%

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Dissolution Behavior of Lipid Monolayer-Coated, Air-Filled Microbubbles: Effect of Lipid Hydrophobic Chain Length

2002

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The gas transport properties and dissolution behavior of the lipid monolayer shell coating a micron-scale bubble (microbubble) are of particular interest for developing stable ultrasound contrast agents, drug delivery vehicles, and efficient oxygen carriers. In the current study, Epstein and Plesset's model for the dissolution of a bubble into a quiescent, degassed medium was modified to include a term for the gas permeation resistance of the lipid shell. The shell resistances for a homologous series of phospholipids were determined by comparing this model to experimental data of lipid-coated, air-filled microbubbles dissolving in a degassed aqueous medium. The gas permeation resistance is a significant factor in controlling the dissolution rate of lipid-coated microbubbles and was found to increase monotonically with lipid hydrophobic chain length. During the dissolution process, lipid was shed from the shell in a continuous manner for short-chain lipids to accommodate the shrinking volume of the gas core. A cyclic process of buckling and lipid shedding was observed for long-chain lipids and a mechanism involving adhesive zippering of apposing monolayers is proposed to describe this phenomenon.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Dissolution Behavior of Lipid Monolayer-Coated, Air-Filled Microbubbles: Effect of Lipid Hydrophobic Chain Length

2002

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“…This also increases the diffusion distances for O 2 /CO 2 , which affects – in accordance to Fick’s law of diffusion – the rates of diffusion of these gases (Weibel et al, 1993; Sznajder, 2001; Hoschele and Mairbaurl, 2003). In addition, the diffusion rate of gases can be influenced by the chemical composition of the alveolar fluid and the surfactant (Ladanyi et al, 1992; Connor et al, 2001). Furthermore, there is evidence that the rate of water evaporation is also influenced by the composition of the alveolar fluid (Rantamaki et al, 2011).…”

Section: Function Malfunction Regulation and Transport Routes Of Tmentioning

confidence: 99%

Why Do We have to Move Fluid to be Able to Breathe?

2012

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The ability to breathe air represents a fundamental step in vertebrate evolution that was accompanied by several anatomical and physiological adaptations. The morphology of the air-blood barrier is highly conserved within air-breathing vertebrates. It is formed by three different plies, which are represented by the alveolar epithelium, the basal lamina, and the endothelial layer. Besides these conserved morphological elements, another common feature of vertebrate lungs is that they contain a certain amount of fluid that covers the alveolar epithelium. The volume and composition of the alveolar fluid is regulated by transepithelial ion transport mechanisms expressed in alveolar epithelial cells. These transport mechanisms have been reviewed extensively. Therefore, the present review focuses on the properties and functional significance of the alveolar fluid. How does the fluid enter the alveoli? What is the fate of the fluid in the alveoli? What is the function of the alveolar fluid in the lungs? The review highlights the importance of the alveolar fluid, its volume and its composition. Maintenance of the fluid volume and composition within certain limits is critical to facilitate gas exchange. We propose that the alveolar fluid is an essential element of the air-blood barrier. Therefore, it is appropriate to refer to this barrier as being formed by four plies, namely (1) the thin fluid layer covering the apical membrane of the epithelial cells, (2) the epithelial cell layer, (3) the basal membrane, and (4) the endothelial cells.

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“…Ladanyi has developed an electrochemical method based on dc and ac polarography to indirectly study the effect of lung surfactant films on oxygen transfer. Using this method, Ladanyi and co-workers qualitatively reported that both dipalmitoyl phosphatidylcholine (DPPC) films (spread at surface tension of 42 and 27 mJ/m 2 , respectively) and lung surfactant extracts 10 presented resistance to oxygen transfer and that the resistance increased with deceasing surface tension …”

Section: Introductionmentioning

confidence: 99%

“…Using this method, Ladanyi and co-workers qualitatively reported that both dipalmitoyl phosphatidylcholine (DPPC) films (spread at surface tension of 42 and 27 mJ/m 2 , respectively) 11 and lung surfactant extracts 10 presented resistance to oxygen transfer and that the resistance increased with deceasing surface tension. 11 More recent studies of the influence of phospholipid films on interfacial oxygen transfer relied on Langmuir-type balances, in which a spread phospholipid monolayer is compressed by a moving barrier and the surface tension is measured by a Wilhelmy plate. [12][13][14][15] Sosnowski et al 12 studied the resistance of spread DPPC films to oxygen transfer.…”

Section: Introductionmentioning

confidence: 99%

Effect of Compressed Bovine Lipid Extract Surfactant Films on Oxygen Transfer

Zuo

Acosta²,

Cox³

et al. 2006

Langmuir

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In the lungs, oxygen transfer from the inspired air to the capillary blood needs to cross the surfactant lining layer of the alveoli. Therefore, the gas transfer characteristics of lung surfactant film are of fundamental physiological interest. However, previous in vitro studies-most relied on the Langmuir-type balance-fail to cover the low surface tension range (i.e., less than the equilibrium surface tension of approximately 25 mJ/m2) due to film leakage. We have recently developed a novel in vitro experimental strategy, the combination of axisymmetric drop shape analysis and captive bubble technique (ADSA-CB), in studying the effect of surfactant films on interfacial gas transfer (Langmuir 2005, 21, 5446). In the present work, ADSA-CB is used as a micro-film-balance to study the effect of compressed bovine lipid extract surfactant (BLES) films on oxygen transfer. A low surface tension ranging from approximately 25 mJ/m2 to 2 mJ/m2 is studied. The experimental results suggest that lung surfactant films at a low surface tension near 2 mJ/m2 provide resistance to oxygen transfer, as indicated by a decrease of 30-50% in the mass transfer coefficient (kL) of oxygen in BLES suspensions with respect to water. At higher surface tension (i.e., >6 mJ/m2), the resistance to oxygen transfer is only modest, i.e., the decrease in kL is less than 20% compared to water. The experimental results suggest that lung surfactant plays a role in oxygen transfer in the pulmonary system.

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Oxygen Transport Through a Model Lung Surfactant Surface Layer: Influence of the Film Compression on the Kinetics

Cited by 3 publications

References 3 publications

Dissolution Behavior of Lipid Monolayer-Coated, Air-Filled Microbubbles: Effect of Lipid Hydrophobic Chain Length

Dissolution Behavior of Lipid Monolayer-Coated, Air-Filled Microbubbles: Effect of Lipid Hydrophobic Chain Length

Why Do We have to Move Fluid to be Able to Breathe?

Effect of Compressed Bovine Lipid Extract Surfactant Films on Oxygen Transfer

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