Mass transport phenomena influence all kinds of bioprocesses, often controlling the rate, the yield, the product spectrum, and the downstream recovery. This article details the mass transfer issues in bioprocessing. Fundamentals of mass transfer are discussed for gas‐liquid, liquid‐liquid, and solid‐liquid systems. Methods for measurement of the overall gas‐liquid volumetric mass transfer coefficient (
k
L
a
L
) are noted. Mass transport behavior is discussed for various kinds of bioreactors: shake flasks, tissue culture flasks, vortex fermenters, stirred fermenters, bubble columns, airlift bioreactors, fluidized beds, packed beds, and other devices. Prediction and estimation of the various mass transfer coefficients are emphasized. Mass transfer within particles is briefly treated as being relevant to immobilized enzyme and cell biocatalysis. Specific considerations are noted for processes such as animal cell culture, microbial fermentations, downstream product recovery, and wastewater treatment. Mass transport in membrane processing is outlined.