Oxygen transfer rate and sophorose lipid production by <i>Candida bombicola</i>

Guilmanov, Vladimir; Ballistreri, Alberto; Impallomeni, Giuseppe; Gross, Richard A.

doi:10.1002/bit.10177

Cited by 67 publications

(43 citation statements)

References 21 publications

(29 reference statements)

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“…Guilmanov et al 67 investigaram o efeito da aeração, por meio de experimentos em frascos agitados, na produção de soforolipídeos por C. bombicola. O maior rendimento foi obtido com a taxa de aeração entre 50 e 80 mM O 2 /l h -1 .…”

Section: Temperatura Ph Aeração E Agitaçãounclassified

Produção de biossurfactante por levedura

Fontes¹,

Amaral²,

Coelho³

2008

Quím. Nova

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Recebido em 18/8/07; aceito em 24/4/08; publicado na web em 16/10/08 BIOSURFACTANTS PRODUCTION BY YEASTS. Biosurfactants are molecules extracellularly produced by bacteria, yeast and fungi that have significant interfacial activity properties. This review focuses on relevant parameters that influence biosurfactant production by yeasts. Many works have investigated the optimization of yeast biosurfactant production, mainly within the last decade, revealing that the potential of such microorganisms is not well explored in the industrial field. The main points to increase the process viability lays on the reduction of the production costs and enhancement of biosynthesis efficiency through optimization the culture conditions (carbon and nitrogen source, pH, aeration, speed agitation) and the selection of inexpensive medium components.Keywords: biosurfactants; yeast; surface-active compounds. introduçãoVários microorganismos possuem capacidade de produzir moléculas com atividade interfacial. 1 Nas últimas décadas tem-se aumentado o interesse em identificar e isolar novos microorganismos produtores de moléculas tenso-ativas que apresentem boas caracterís-ticas surfactantes, como baixa concentração micelar crítica (CMC), baixa toxicidade, alta atividade de emulsificação, dentre outras. 2 A maioria dos biossurfactantes microbianos relatados na literatura é de origem bacteriana. As bactérias produtoras mais reportadas são dos gêneros: Pseudomonas sp., Acinetobacter sp., Bacillus sp. e Arthrobacter sp.. 3 Entretanto, a grande maioria dos biossurfactantes de origem bacteriana não é adequada para utilização na indústria alimentícia, devido a sua possível natureza patogênica. 4 As leveduras também têm sido estudadas para a produção de emulsificadores. Entre as leveduras, espécies de Candida e Yarrowia têm sido largamente estudadas e empregadas com sucesso na produção de biossurfactanes. A Tabela 1 apresenta as espécies de levedura mais estudadas e os respectivos biossurfactantes produzidos.Uma grande vantagem do uso de leveduras reside no status GRAS (generally regarded as safe) que muitas delas apresentam, como Yarrowia lipolytica, Saccharomyces cerevisiae e Kluyveromyces lactis. Organismos com status GRAS não apresentam riscos de toxicidade e patogenicidade, o que permite sua utilização para aplicações nas indústrias de alimentos e farmacêutica. 5 Alguns microorganismos podem produzir biossurfactantes quando crescem em diferentes substratos, variando desde carboidratos até hidrocarbonetos. O uso de diferentes fontes de carbono altera a estrutura dos biossurfactantes produzidos e, conseqüentemente, suas propriedades emulsificantes. Estas mudanças podem ser benéficas quando se desejam propriedades específicas para uma aplicação direcionada. 26 Diversos são os estudos realizados por vários autores 18,27,28 na produção de biossurfactantes, envolvendo propriedades físico-químicas.A maioria dos biossurfactantes é geralmente produzida quando as culturas alcançam a fase estacionária de crescimento. 29 Porém algumas espécies podem...

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Section: Temperatura Ph Aeração E Agitaçãounclassified

Produção de biossurfactante por levedura

Fontes¹,

Amaral²,

Coelho³

2008

Quím. Nova

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“…Because of its high product yield, Candida bombicola has been the favored organism used in the study of sophorolipid production (Daniel et al 1998;Guilmanov et al 2002). Product yields of 100 g/l or more are routinely obtained under laboratory conditions using glucose and a lipid substrate such as oleic acid or rapeseed oil as feedstocks.…”

Section: Introductionmentioning

confidence: 99%

Simplified soy molasses-based medium for reduced-cost production of sophorolipids by Candida bombicola

et al. 2007

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A simplified medium containing only soy molasses and oleic acid as ingredients was developed for the production of sophorolipids (SLs) from Candida bombicola. We achieved a product yield of 53 +/- 3 g of purified sophorolipids per liter of starting culture volume, which is 71 +/- 4% of the yield obtained with growth medium that also additionally contains the costly yeast extract and urea as nitrogen source. The large majority of the SL components existed in the lactone form (87%), and the predominant component is SL containing (omega-1)-hydroxyoleic acid as the lipid moiety. The study demonstrated for the first time the usefulness of the low-value soy molasses as a combined nitrogen- and carbon-source for SL production at a reduced cost.

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“…A high concentration of alcohol (including methanol) can inhibit many microorganisms primarily by disrupting lipid metabolism and hence membrane formation and fluidity (Ingram and Buttke 1985). Since SL synthesis is dependent on high oxygenation levels (Guilmanov et al 2002), it is likely that the rate at which air was sparged through each system to oxygenate the media for SL production was sufficient to evaporate much of the methanol to levels that were tolerable for microbial growth resulting in similar cell yields in all cultures after 7 days. This was, in fact, proven by monitoring the methanol concentrations in each fermenter over the course of the fermentations (Fig.…”

Section: Resultsmentioning

confidence: 98%

The influence of increasing media methanol concentration on sophorolipid biosynthesis from glycerol-based feedstocks

Ashby

Solaiman²

2010

Biotechnol Lett

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Candida bombicola, a known producer of sophorolipids (SLs; glycolipid surfactants), was grown on glycerol and oleic acid with up to 1.5% (v/v) methanol in the fermentation growth media to assess the effects of methanol presence on SL synthesis and structural distribution. Increasing methanol concentrations had little effect on the growth of the organism resulting in average cell dry weights (CDW; after SL separation) of 20.8 ± 0.7 g/l between 0 and 1.5% methanol. However, increasing methanol concentrations decreased SL production by 56% (from 12.7 to 5.6 g/l at 1.5% methanol) which translated to SL yields on a cellular basis of between 0.60 g SL/g cells (in the absence of methanol) to 0.27 g SL/g cells (in the presence of 1.5% methanol). LC/MS revealed that increased methanol concentrations also resulted in larger concentrations (up to 20 mol%) of free acid SLs but had little effect on the ratios of diacetylated SL lactones synthesized with palmitic acid (4 mol%), linoleic acid (3 mol%), oleic acid (80 mol%), and stearic acid (13 mol%) as the hydrophobic moieties.

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Oxygen transfer rate and sophorose lipid production by Candida bombicola

Cited by 67 publications

References 21 publications

Produção de biossurfactante por levedura

Produção de biossurfactante por levedura

Simplified soy molasses-based medium for reduced-cost production of sophorolipids by Candida bombicola

The influence of increasing media methanol concentration on sophorolipid biosynthesis from glycerol-based feedstocks

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