Oxygen tension and medium type actions on blastocyst development and interferon-tau secretion in cattle

Rodina, T. M.; Cooke, Flavia N.T.; Hansen, Peter J.; Ealy, Alan D.

doi:10.1016/j.anireprosci.2008.02.014

Cited by 31 publications

(29 citation statements)

References 57 publications

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“…Previous work consistently observed little to no mitogenic effect of boFGF2 (Michael et al 2006, Rodina et al 2008. One interpretation of these findings is that the FGFs under investigation are likely not serving an active role in regulating cell proliferation rate early in conceptus development.…”

Section: Fgfs In Bovine Conceptusesmentioning

confidence: 60%

“…This laboratory previously described a role for FGF2 in stimulating IFNT production in bovine conceptuses (Michael et al 2006, Rodina et al 2008. Initially, the uterine epithelium was proposed as the primary site of FGF2 production in cattle (Michael et al 2006), but work contained herein and other findings (Daniels et al 2000, Lazzari et al 2002, Ocon-Grove et al 2008 establish that bovine conceptuses also produce FGF2.…”

Section: Discussionmentioning

confidence: 99%

“…In one study, blastocysts were incubated at 38.5 8C in 5% CO 2 /5%O 2 /90%N 2 for 24 h, then washed once in PBS/PVP, snap-frozen in liquid nitrogen and stored at K80 8C. In a companion study, blastocysts were incubated at the aforementioned conditioned for 48 h then were washed once in PBS/PVP and fixed by incubation in 4%(w/v) paraformaldehyde (Polysciences Inc., Warrington, PA, USA) for 10 min and processed for determining blastomere numbers with Hoechst 33342 staining and epifluorescence microscopy (Rodina et al 2008). Number of Hoechst-positive nuclei was quantified with NIS-Elements software (Nikon Instruments Inc, Melville, NY, USA).…”

Section: Bovine In Vitro Embryo Productionmentioning

confidence: 99%

“…Individual blastocysts were placed in 30 ml drops Medium 199 (M199) containing 2.5% (v/v) fetal bovine serum (FBS; Invitrogen Corp.) and 10 mM Gentamycin (Sigma Chemical Co.; Rodina et al 2008). The stage of blastocyst development (non-expanded or expanded) was used to balance treatment combinations.…”

Section: Bovine In Vitro Embryo Productionmentioning

confidence: 99%

“…FGF2 is expressed by the luminal and glandular epithelium and detected in the uterine lumen of cows and ewes throughout the estrous cycle and early pregnancy (Michael et al 2006, Ocon-Grove et al 2008. Studies using a bovine trophectoderm cell line (CT1) and in vitro-produced (IVP) bovine blastocysts reveal that supplementation with FGF2 increases IFNT mRNA and protein levels (Michael et al 2006, Rodina et al 2008.…”

Section: Introductionmentioning

confidence: 99%

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Several fibroblast growth factors are expressed during pre-attachment bovine conceptus development and regulate interferon-tau expression from trophectoderm

Cooke¹,

Pennington²,

Yang³

et al. 2009

Reproduction

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The trophectoderm-derived factor interferon tau (IFNT) maintains the uterus in a pregnancy-receptive state in cattle and sheep. Fibroblast growth factors (FGFs) are implicated in regulating IFNT expression and potentially other critical events associated with early conceptus development in cattle. The overall objectives of this work were to identify the various FGFs and FGF receptors (FGFRs) expressed in elongating pre-attachment bovine conceptuses and determine if these FGFs regulate conceptus development and/or mediate IFNT production. In vitro-derived bovine blastocysts and in vivo-derived elongated conceptuses collected at day 17 of pregnancy express at least four FGFR subtypes (R1c, R2b, R3c, R4). In addition, transcripts for FGF1, 2, and 10 but not FGF7 are present in elongated bovine conceptuses. The expression pattern of FGF10 most closely resembled that of IFNT, with both transcripts remaining low in day 8 and day 11 conceptuses and increasing substantially in day 14 and day 17 conceptuses. Supplementation with recombinant FGF1, 2 or 10 increased IFNT mRNA levels in bovine trophectoderm cells and bovine blastocysts and increased IFNT protein concentrations in trophectoderm-conditioned medium. Blastocyst development was not affected by any of the FGFs. In summary, at least four FGFRs reside in pre-and peri-attachment bovine conceptuses. Moreover, conceptuses express at least three candidate FGFs during elongation, the time of peak IFNT expression. These findings provide new insight for how conceptus-derived factors such as FGF1, 2, and 10 may control IFNT expression during early pregnancy in cattle.

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Section: Fgfs In Bovine Conceptusesmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Bovine In Vitro Embryo Productionmentioning

confidence: 99%

Section: Bovine In Vitro Embryo Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Several fibroblast growth factors are expressed during pre-attachment bovine conceptus development and regulate interferon-tau expression from trophectoderm

Cooke¹,

Pennington²,

Yang³

et al. 2009

Reproduction

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Transcriptomic signature to oxidative stress exposure at the time of embryonic genome activation in bovine blastocysts

Cagnone

Sirard

2013

Molecular Reproduction Devel

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In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions. Two pro-oxidant agents, one that acts extracellularly by promoting reactive oxygen species (ROS) production (0.01 mM 2,2'-azobis (2-amidinopropane) dihydrochloride [AAPH]) or another that acts intracellularly by inhibiting glutathione synthesis (0.4 mM buthionine sulfoximine [BSO]) were added separately to in vitro culture media from Day 3 (8-16-cell stage) onward. Transcriptomic analysis was then performed on resulting Day-7 blastocysts. In the literature, these two pro-oxidant conditions were shown to induce delayed degeneration in a proportion of Day-8 blastocysts. In our experiment, no morphological difference was visible, but AAPH tended to decrease the blastocyst rate while BSO significantly reduced it, indicating a differential impact on the surviving population. At the transcriptomic level, blastocysts that survived either pro-oxidant exposure showed oxidative stress and an inflammatory response (ARRB2), although AAPH induced higher disturbances in cellular homeostasis (SERPINE1). Functional genomics of the BSO profile, however, identified differential expression of genes related to glycine metabolism and energy metabolism (TPI1). These differential features might be indicative of pre-degenerative blastocysts (IGFBP7) in the AAPH population whereas BSO exposure would select the most viable individuals (TKDP1). Together, these results illustrate how oxidative disruption of pre-attachment development is associated with systematic up-regulation of several metabolic markers. Moreover, it indicates that a better capacity to survive anti-oxidant depletion may allow for the survival of blastocysts with a quieter metabolism after compaction.

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Embryo responses to stress induced by assisted reproductive technologies

Ramos‐Ibeas

Heras

Gómez-Redondo

et al. 2019

Molecular Reproduction Devel

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Assisted reproductive technology (ART) has led to the birth of millions of babies. In cattle, thousands of embryos are produced annually. However, since the introduction and widespread use of ART, negative effects on embryos and offspring are starting to emerge. Knowledge so far, mostly provided by animal models, indicates that suboptimal conditions during ART can affect embryo viability and quality, and may induce embryonic stress responses. These stress responses take the form of severe gene expression alterations or modifications in critical epigenetic marks established during early developmental stages that can persist after birth. Unfortunately, while developmental plasticity allows the embryo to survive these stressful conditions, such insult may lead to adult health problems and to long-term effects on offspring that could be transmitted to subsequent generations. In this review, we describe how in mice, livestock, and humans, besides affecting the development of the embryo itself, ART stressors may also have significant repercussions on offspring health and physiology. Finally, we argue the case that better control of stressors during ART will help improve embryo quality and offspring health. K E Y W O R D S assisted reproductive technologies, bovine, embryo, human, mouse, stress

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Oxygen tension and medium type actions on blastocyst development and interferon-tau secretion in cattle

Cited by 31 publications

References 57 publications

Several fibroblast growth factors are expressed during pre-attachment bovine conceptus development and regulate interferon-tau expression from trophectoderm

Several fibroblast growth factors are expressed during pre-attachment bovine conceptus development and regulate interferon-tau expression from trophectoderm

Transcriptomic signature to oxidative stress exposure at the time of embryonic genome activation in bovine blastocysts

Embryo responses to stress induced by assisted reproductive technologies

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