Oxygen tension affects histone remodeling of in vitro–produced embryos in a bovine model

Gaspar, Roberta Cordeiro; Arnold, Daniel; Corrêa, Carolina Alves Pereira; Rocha, Carlos V. da; Penteado, João C.T.; Collado, Maite del; Vantini, Roberta; Garcia, J. M.; Lopes, Flávia L.

doi:10.1016/j.theriogenology.2015.01.002

Cited by 37 publications

(14 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…COCs with homogenous cytoplasm and at least three layers of compact cumulus cells were selected. The selected COCs were washed twice in TCM-199 medium (Gibco BRL, Grand Island, NY, USA) supplemented with 5 mM sodium bicarbonate, 10 mM HEPES sodium salt (Sigma), 10 mM HEPES free acid (Sigma), 10% fetal bovine serum (FBS; Cripion, Andradina, SP, Brazil), 0.20 mM sodium pyruvate, and 83.4-mg/mL amikacin (Instituto Biochimico, Rio de Janeiro, Brazil), and once in TCM-199 maturation medium (Gibco BRL) buffered with 25 mM sodium bicarbonate and supplemented with 10% FBS (Cripion), 1.0 mg/mL FSH (Folltropin; Bioniche Animal Health, Belleville, Canada), 50 mg/mL hCG (Vetercor; Intervet, São Paulo, Brazil), 1.0 mg/mL estradiol, 0.20 mM sodium pyruvate, and 83.4 mg/mL amikacin (Instituto Biochimico), according to Gaspar et al [25]. Groups of 20 to 25 COCs were cultured in 100-mL microdroplets of maturation medium under sterile mineral oil for 24 hours at 38.5 C in a humidified atmosphere of 5% CO 2 in air.…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

et al. 2017

Self Cite

View full text Add to dashboard Cite

Cryopreservation of bull semen is a common biotechnology procedure in cattle breeding. However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. The removal of SP may improve the quality of frozen bull semen. The objective of this study was to evaluate the effect of SP removal from the ejaculate on the cryopreservation of semen from 38 Nellore bulls collected by electroejaculation. After collection, the ejaculate was divided into three aliquots: (1) control (N) diluted to a concentration of 60 × 10 spermatozoa/mL and frozen with SP; (2) centrifugation (C) at ×600g for 10 minutes and the pellet resuspended and frozen at the same concentration as N; and (3) filtration (F) through SpermFilter and sperm recovered and frozen at the same concentration as N. After thawing, sperm kinetics, plasma and acrosome membrane integrity, mitochondrial membrane potential, oxidative stress, and in vitro fertility were evaluated. Statistical analysis was performed using the SAS 9.2 package, and differences were considered significant when P < 0.05. Higher average path velocity and straight-line velocity were observed in the groups submitted to SP removal compared to the control group (P < 0.01). In contrast, filtered samples exhibited higher beat cross frequency, straightness, and linearity compared to the other groups. Plasma membrane integrity was reduced when SP was removed, but lower oxidative stress was observed in groups C and F (34.91 ± 2.95% and 31.63 ± 2.95%, respectively) compared to group N (57.39 ± 2.95%). However, the percentage of hatched blastocysts was similar in the N and F groups (21.22 ± 1.05% and 24.00 ± 1.05%, respectively) and higher compared to group C (18.83 ± 1.05%). In conclusion, removal of SP by centrifugation for bull semen freezing reduced the rate of in vitro-produced embryos, whereas filtration of prefrozen semen was found to be an efficient alternative in terms of semen freezability and in vitro production of bovine embryos.

show abstract

Section: In Vitro Embryo Productionmentioning

confidence: 99%

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Physiological oxygen concentrations are around 2-8% in the reproductive tract (Fischer and Bavister, 1993;Mastroianni and Jones, 1965), and studies on multiple mammalian species have shown that culture in 20% oxygen is detrimental for embryo development, resulting in slower cleavage timings (Wale and Gardner, 2010;Weinerman et al, 2016), lower blastocyst rates and cell numbers (Batt et al, 1991;Quinn and Harlow, 1978;Tervit et al, 1972;Thompson et al, 1990;Whitten, 1971), increased apoptosis (Van Soom et al, 2002;Yuan et al, 2003), more frequent aneuploidy (Bean et al, 2002), more DNA damage (Kitagawa et al, 2004;Takahashi et al, 2000) and higher hydrogen peroxide concentrations (Goto et al, 1993;Kitagawa et al, 2004;Kwon et al, 1999). Atmospheric oxygen in culture is also associated with differential preimplantation gene expression (Harvey et al, 2004;Kind et al, 2005;Meuter et al, 2014;Rinaudo et al, 2006), histone remodelling and global methylation (Gaspar et al, 2015;Li et al, 2014), alterations of the proteome (Katz-Jaffe et al, 2005), secretome (Kubisch and Johnson, 2007;Rodina et al, 2009) and metabolism (Khurana and Wales, 1989;Wale and Gardner, 2012) compared with 5% oxygen. These changes result in perturbed post-implantation development following culture in 20% oxygen (de Waal et al, 2014;Fischer-Brown et al, 2005;Karagenc et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Kelley

Gardner

2016

Reproductive BioMedicine Online

View full text Add to dashboard Cite

Embryos are routinely cultured individually, although this can reduce blastocyst development. Culture in atmospheric (20%) oxygen is also common, despite multiple detrimental effects on embryos. Although frequently occurring together, the consequences of this combination are unknown. Mouse embryos were cultured individually or grouped, under physiological (5%) or atmospheric (20%) oxygen. Embryos were assessed by time-lapse and blastocyst cell allocation. Compared with the control group (5% oxygen group culture), 5-cell cleavage (t5) was delayed in 5% oxygen individual culture and 20% oxygen group culture (59.91 ± 0.23, 60.70 ± 0.29, 63.06 ± 0.32 h post-HCG respectively, P < 0.05). Embryos in 20% oxygen individual culture were delayed earlier (3-cell cleavage), and at t5 cleaved later than embryos in other treatments (66.01 ± 0.40 h, P < 0.001), this delay persisting to blastocyst hatching. Compared with controls, hatching rate and cells per blastocyst were reduced in 5% oxygen single culture and 20% oxygen group culture (134.1 ± 3.4, 104.5 ± 3.2, 73.4 ± 2.2 cells, P < 0.001), and were further reduced in 20% oxygen individual culture (57.0 ± 2.8 cells, P < 0.001), as was percentage inner cell mass. These data indicate combining individual culture and 20% oxygen is detrimental to embryo development.

show abstract

“…38 Moreover, the oxygen level affected histone post transcription modifications on the chromatin of blastocysts which might impact gene expression, while the presence of serum in the media was without effects on chromatin despite a higher developmental rate in vitro. 39 Indeed the culture conditions like higher glucose can stimulate, sometime excessively, embryo metabolism, 40 creating a phenotype similar to the ones induced by excess intra-or extracellular free radical species during that same period. 41 It is interesting to analyze the different responses to metabolic stresses like glucose, free radicals or lipids to realize that the most obvious victim is the mitochondria.…”

Section: Resultsmentioning

confidence: 99%

The influence of in vitro fertilization and embryo culture on the embryo epigenetic constituents and the possible consequences in the bovine model

Sirard

2017

J Dev Orig Health Dis

View full text Add to dashboard Cite

Medically assisted reproductive technologies, such as in vitro embryo production, are increasingly being used to palliate infertility. Eggs are produced following a hormonal regimen that stimulates the ovaries to produce a large number of oocytes. Collected oocytes are then fertilized in vitro and allowed to develop in vitro until they are either frozen or transferred to mothers. There are controversial reports on the adverse impacts of these technologies on early embryos and their potential long-term effects. Using newly developed technological platforms that enable global gene expression and global DNA methylation profiling, we evaluated gene perturbations caused by such artificial procedures. We know that cells in the early embryo produce all cells in the body and are able to respond to their in vitro environment. However, it is not known whether gene perturbations are part of a normal response to the environment or are due to distress and will have long-term impacts. While the mouse is an established genetic model used for quality control of culture media in clinics, the bovine is a large mono-ovulating mammal with similar embryonic kinetics as humans during the studied developmental window. These model systems are critical to understand the effects of assisted reproduction without the confounding impact of infertility and without the limitations imposed by the scarcity of donated human samples and ethical issues. The data presented in this review come mostly from our own experimentation, publications, and collaborations. Together they demonstrate that the in vitro environment has a significant impact on embryos at the transcriptomic level and at the DNA methylation level.

show abstract

Oxygen tension affects histone remodeling of in vitro–produced embryos in a bovine model

Cited by 37 publications

References 65 publications

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

The influence of in vitro fertilization and embryo culture on the embryo epigenetic constituents and the possible consequences in the bovine model

Contact Info

Product

Resources

About