Oxygen Supply and Energy-yielding Substrates for Nitrogen Fixation (Acetylene Reduction) by Bacteroid Preparations

Trinchant, Jean-Charles; Birot, A.; Rigaud, J.

doi:10.1099/00221287-125-1-159

Cited by 26 publications

(29 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16) or soybean bacteroids (Trinchant et al, 1981). The strict 0, requirement with glucose as substrate was also confirmed when 0, was either freely dissolved or supplied by oxyleghaemoglobin.…”

Section: Discussionmentioning

confidence: 77%

“…The strict 0, requirement with glucose as substrate was also confirmed when 0, was either freely dissolved or supplied by oxyleghaemoglobin. It could be responsible for the commonly reported inefficiency of glucose in supporting CzH2 reduction, which may be explained by inadequate conditions of incubation as previously suggested (Trinchant & Rigaud, 1979;Trinchant et al, 1981).…”

Section: Discussionmentioning

confidence: 88%

“…An alteration of the sugar transport system during the bacteroid preparation might also play a part in hexose inefficiency (Dilworth & Glenn, 1984). However, glucose acts as an energy-yielding substrate for bacteroids isolated from French-bean and soybean nodules (Trinchant et al, 1981(Trinchant et al, , 1983) with O2 tensions lower than those required with organic acids such as succinate; the O2 requirement appears to be directly related to the stimulation by these substrates of bacteroid respiration (Bergersen & Trinchant, 1985).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Acetylene Reduction by Bacteroids Isolated from Stem Nodules of Sesbania rostrata. Specific Role of Lactate as an Energy-yielding Substrate

Trinchant

Rigaud

1987

Microbiology

Self Cite

View full text Add to dashboard Cite

Bacteroids isolated from stem nodules of Sesbania were able to reduce C2H2 when glucose or succinate was the energy-yielding substrate. To be efficient, glucose always required low O2 concentrations, both with free O2 and with O2 delivered by leghaemoglobin extracted from French-bean nodules. Lactate detected in the stem nodule cytosol supported C2H2 reduction by bacteroid preparations, but the O2 tensions required for optimal activity were always higher than those defined for glucose, as predicted by the model of Bergersen & Trinchant (J. theor. Biol. 115, 93-102, 1985). In contrast, lactate was only present at very low concentrations in the cytosol of French-bean nodules and was unable to support C2Hz reduction by bacteroids isolated from these nodules. Lactate dehydrogenase (EC 1 . 1 . 1 .27) extracted from stem nodule bacteroids and free-living cells of Rhizobium sp. (Sesbania) exhibited a higher affinity for lactate than did the enzyme from Rhizobium meliloti, R. leguminosarum biovar phaseoli or Bradyrhizobium and their symbiotic forms. Lactate appears to be a specific energy-yielding substrate supporting N 2 fixation by Sesbania bacteroids and could play a major role when fermentative processes take place in the nodules.

show abstract

Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acetylene Reduction by Bacteroids Isolated from Stem Nodules of Sesbania rostrata. Specific Role of Lactate as an Energy-yielding Substrate

Trinchant

Rigaud

1987

Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our results are in agreement with that found by them. Furthermore, the presence of Azospirillum and Azotobacter in soils has been shown to be pH dependent, but it has been pointed out that roots of grasses provide optimal conditions for the bacteria even at low soil pH values (41,42). Dinitrogen fixing organisms occurring in soil remote from the roots are probably primarily limited by carbon nutrition, pH limitations being secondary.…”

Section: Resultsmentioning

confidence: 99%

Dinitrogen fixation by Azotobacter chroococcum and its isocitrate dehydrogenase activity: A quick method for screening nitrogen fixing strains.

Varma¹,

Rao²

1983

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

Nitrogenase activity in the rhizosphere of sugarcane (Saccharum officinarum Linn.), and its associated soil was measured by reduction of acetylene. Zero order kinetics and first order kinetics of acetylene reduction (C2H2---*C,H4) were used to describe the activity of N2 fixers from these soils. Arranged in descending order of nitrogen-fixing (acetylene-reducing) potential, the sites examined were highland (arable), mesic meadow, peat polygon trough, transition zone between mesic meadow and peat polygon trough, lowland (flooded with water) and virgin flooded land. On most of the land, sugarcane has been under cultivation for centuries. In total, 9 acetylene reducers were isolated, the remaining being oligonitrophiles. In addition to using the saturation kinetic values to describe the activity of dinitrogen fixers, first order kinetics of several potential energy substrates were also compared. Carbohydrate concentrations (glucose, maltose, arabinose, mannose and raffinose) between 25 and 100 ,ug/ml were used since the MichaelisMenten plot indicates that between this range first order kinetics were measured. The results indicated that various carbohydrate additions had different effects on C2H2 reduction. Because of the relatively high temperature requirements for Azotobacter chroococcum development, tropical areas appear more favorable for a higher incidence of this bacterium. Optimal temperatures for acetylene reduction were between 25°-30°. Acetylene reduction was pH-dependent, the optimum occurring between pH 6.4-7.5. Nitrogenase activity was almost completely inhibited by air and anaerobic conditions, and was optimum at around p02 0.06 atm. The reduction of 2, 3, 5-triphenyl tretrazolium chloride to triphenyl

show abstract

“…Besides the essential role played by organic acids to support N2 fixation (Day and Copeland, 1991;Streeter, 1991), studies have explored the ability of other molecules such as Glc (Trinchant et al, 1981; …”

mentioning

confidence: 99%

Acetylene Reduction by Symbiosomes and Free Bacteroids from Broad Bean (Vicia faba L.) Nodules (Role of Oxalate)

1994

Self Cite

View full text Add to dashboard Cite

and Rigaud, 1987), glutamate (Kahn et al., 1985), or Pro (Kohl et al., 1988) to be potential sources of energy for bacteroids. However, the preparation of intact symbiosomes from different legumes (Herrada et al., 1989; Udvardi and D~~, 1989;Rosendahl et 1992) has provided interesting infomation conceming the selectivity of the PBM and has lead to a reappraisal of the tyPe of substrates available to the bacteroid' Thusf the absence Of carriers for glutamate (Udvardi et 1988; Herrada et 1989) Or Pro (Udvardi et al.f 1990) on the PBM stronglY lifits the possibilitY that these mokcules are SOurceS of energy for bacteroids. In the same way, the abundance of certain carbohydrates in the nodule cytosol does not necessarily imply that it is utilized by the bacteroid. This situation occurs for &ate and benzoate, which is found in soybean (Glycine max L.) (Streeter, 1991), but these molecules are unable to cross the PBM (Herrada et al., 1989).In this paper we report the presence of high concentrationsOf oxalate in broad bean (Vicia fabQ L.1 nodules and examine the ability of oxalate to CrOSS the PBM and the bacteroid membranes. In addition, we studied the capacity for oxalate to support C2H2 reduction and respiration in symbiosomes and free bacteroids in the presence of leghemoglobin.We report the presence of oxalate in the organic acid fraction of broad bean (Vicia faba 1.) nodule cytosol. Using both highperformance liquid chromatography and enzymic assays, high levels of oxalate were detected (70.4 f 2.4 mM). The Rhizobium-legume symbiosis is characteked by numerous and complex interactions between the two partners. Besides the exchange of signals during the recognition (Long, 1989; Dénarié et al., 1992) and the differentiation of nodules (Truchet et al., 1991), the microsymbionts receive reduced C and, in retum, provide the host cell with ammonia. The infected cells contain large numbers of bacteroids that express nitrogenase, and a piasmalemma-type membrane that separates the bacteroids from the host cytosol. This PBM defines the bacteroid-containing compartment known as a 'symbiosome" (Roth et al., 1988).Studies of N2 fixation at the cellular leve1 in nodules have involved, until now, isolation of bacteroids and the provision of succinate or malate as the energy-yielding substrates (Bergersen and Tumer, 1975b). Besides the essential role played by organic acids to support N2 fixation (Day and Copeland, 1991; Streeter, 1991), studies have explored the ability of other molecules such as Glc (Trinchant et al., 1981; Trinchant MATERIALS AND METHODS ,,iant CultureBroad beans (Vicia faba L. var minor cv Soravi) were grown in a glasshouse in a sand:vermiculite mixture (3:1, v/v) under the conditions reported elsewhere (Guérin et al., 1990). Seedlings were inoculated 5 and 7 d after planting with a mixture of three strains of Rhizobium leguminosarum bv uiciae (FH 25, FH 34, and FH 20S1), kindly provided by N. Amarger (Institut National de la Recherche Agronomique, Dijon, France). Nodules appeared on the roots abo...

show abstract

Oxygen Supply and Energy-yielding Substrates for Nitrogen Fixation (Acetylene Reduction) by Bacteroid Preparations

Cited by 26 publications

References 19 publications

Acetylene Reduction by Bacteroids Isolated from Stem Nodules of Sesbania rostrata. Specific Role of Lactate as an Energy-yielding Substrate

Acetylene Reduction by Bacteroids Isolated from Stem Nodules of Sesbania rostrata. Specific Role of Lactate as an Energy-yielding Substrate

Dinitrogen fixation by Azotobacter chroococcum and its isocitrate dehydrogenase activity: A quick method for screening nitrogen fixing strains.

Acetylene Reduction by Symbiosomes and Free Bacteroids from Broad Bean (Vicia faba L.) Nodules (Role of Oxalate)

Contact Info

Product

Resources

About