Oxygen Sensing, Uptake, Delivery, Consumption and Related Disorders

Fähling, Michael; Persson, Pontus B.

doi:10.1111/j.1748-1716.2012.02432.x

Cited by 3 publications

(2 citation statements)

References 32 publications

(43 reference statements)

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“…Historically, a variety of acute and chronic O 2 ‐sensing mechanisms have been proposed. These include increases, or paradoxically decreases, in reactive oxygen species (ROS), activation or inactivation of metabolites, for example, nitric oxide, prostanoids, etc, AMP‐activated protein kinase, ion channels, Na,K ATPase, haeme and other proteins and the hypoxia inducible factors …”

Section: Introductionmentioning

confidence: 99%

Extended hypoxia‐mediated H₂S production provides for long‐term oxygen sensing

et al. 2019

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Aim Numerous studies have shown that H2S serves as an acute oxygen sensor in a variety of cells. We hypothesize that H2S also serves in extended oxygen sensing. Methods Here, we compare the effects of extended exposure (24‐48 hours) to varying O2 tensions on H2S and polysulphide metabolism in human embryonic kidney (HEK 293), human adenocarcinomic alveolar basal epithelial (A549), human colon cancer (HTC116), bovine pulmonary artery smooth muscle, human umbilical‐derived mesenchymal stromal (stem) cells and porcine tracheal epithelium (PTE) using sulphur‐specific fluorophores and fluorometry or confocal microscopy. Results All cells continuously produced H2S in 21% O2 and H2S production was increased at lower O2 tensions. Decreasing O2 from 21% to 10%, 5% and 1% O2 progressively increased H2S production in HEK293 cells and this was partially inhibited by a combination of inhibitors of H2S biosynthesis, aminooxyacetate, propargyl glycine and compound 3. Mitochondria appeared to be the source of much of this increase in HEK 293 cells. H2S production in all other cells and PTE increased when O2 was lowered from 21% to 5% except for HTC116 cells where 1% O2 was necessary to increase H2S, presumably reflecting the hypoxic environment in vivo. Polysulphides (H2Sn, where n = 2‐7), the key signalling metabolite of H2S also appeared to increase in many cells although this was often masked by high endogenous polysulphide concentrations. Conclusion These results show that cellular H2S is increased during extended hypoxia and they suggest this is a continuously active O2‐sensing mechanism in a variety of cells.

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Section: Introductionmentioning

confidence: 99%

Extended hypoxia‐mediated H₂S production provides for long‐term oxygen sensing

et al. 2019

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“…in Kaelin 2008). HIFs are hetero-dimers composed of one of three a-subunits, which are regulated by oxygen-dependent proteolysis, and of a constitutive b-subunit (reviews in Maxwell 2004, Metzen & Ratcliffe 2004, Semenza 2004, F€ ahling & Persson 2012. The most studied subunits, HIF-1a and -2a , are structurally similar, but differ with respect to their required hypoxic stimuli, activation thresholds, cell type and organ specificity, target genes and functional properties (Rosenberger et al 2002, Wiesener et al 2003.…”

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Action of hypoxia‐inducible factor in liver and kidney from mice with Pax8‐rtTA‐based deletion of von Hippel‐Lindau protein

et al. 2013

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Combined Impact of Heart Rate Sensor Placements with Respiratory Rate and Minute Ventilation on Oxygen Uptake Prediction

Lu,

Yang,

Tao

et al. 2024

Sensors

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Oxygen uptake (V˙O2) is an essential metric for evaluating cardiopulmonary health and athletic performance, which can barely be directly measured. Heart rate (HR) is a prominent physiological indicator correlated with V˙O2 and is often used for indirect V˙O2 prediction. This study investigates the impact of HR placement on V˙O2 prediction accuracy by analyzing HR data combined with the respiratory rate (RESP) and minute ventilation (V˙E) from three anatomical locations: the chest; arm; and wrist. Twenty-eight healthy adults participated in incremental and constant workload cycling tests at various intensities. Data on V˙O2, RESP, V˙E, and HR were collected and used to develop a neural network model for V˙O2 prediction. The influence of HR position on prediction accuracy was assessed via Bland–Altman plots, and model performance was evaluated by mean absolute error (MAE), coefficient of determination (R2), and mean absolute percentage error (MAPE). Our findings indicate that HR combined with RESP and V˙E (V˙O2HR+RESP+V˙E) produces the most accurate V˙O2 predictions (MAE: 165 mL/min, R2: 0.87, MAPE: 15.91%). Notably, as exercise intensity increases, the accuracy of V˙O2 prediction decreases, particularly within high-intensity exercise. The substitution of HR with different anatomical sites significantly impacts V˙O2 prediction accuracy, with wrist placement showing a more profound effect compared to arm placement. In conclusion, this study underscores the importance of considering HR placement in V˙O2 prediction models, with RESP and V˙E serving as effective compensatory factors. These findings contribute to refining indirect V˙O2 estimation methods, enhancing their predictive capabilities across different exercise intensities and anatomical placements.

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Oxygen Sensing, Uptake, Delivery, Consumption and Related Disorders

Cited by 3 publications

References 32 publications

Extended hypoxia‐mediated H₂S production provides for long‐term oxygen sensing

Extended hypoxia‐mediated H₂S production provides for long‐term oxygen sensing

Action of hypoxia‐inducible factor in liver and kidney from mice with Pax8‐rtTA‐based deletion of von Hippel‐Lindau protein

Combined Impact of Heart Rate Sensor Placements with Respiratory Rate and Minute Ventilation on Oxygen Uptake Prediction

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Oxygen Sensing, Uptake, Delivery, Consumption and Related Disorders

Cited by 3 publications

References 32 publications

Extended hypoxia‐mediated H2S production provides for long‐term oxygen sensing

Extended hypoxia‐mediated H2S production provides for long‐term oxygen sensing

Action of hypoxia‐inducible factor in liver and kidney from mice with Pax8‐rtTA‐based deletion of von Hippel‐Lindau protein

Combined Impact of Heart Rate Sensor Placements with Respiratory Rate and Minute Ventilation on Oxygen Uptake Prediction

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Extended hypoxia‐mediated H₂S production provides for long‐term oxygen sensing

Extended hypoxia‐mediated H₂S production provides for long‐term oxygen sensing