Oxygen radical-dependent expression of CXC chemokines regulate ischemia/reperfusion-induced leukocyte adhesion in the mouse colon

P-selectin glycoprotein ligand-1 regulates chemokine-dependent leukocyte recruitment in colonic ischemia-reperfusion

“…Challenge with MIP-2 and antibodies had no signifi cant effect on peripheral leukocyte count or hemodynamic parameters (Table 1 and 2). Knowing that MIP-2 plays a key role in colonic I/R [19], it was of great interest to also examine the function of PSGL-1 in I/R-induced leukocyte rolling and adhesion in the colon. Indeed, the colonic content of MIP-2 increased signifi cantly from 4.7 ± 0.7 pg/mg in sham operated animals to 65.8 ± 7.9 pg/mg, after I/R (P < 0.05 vs sham).…”

“…An important role of the CXC chemokines MIP-2 and KC in colonic I/R was recently forwarded by Riaz et al [19] although the detailed adhesive mechanisms regulating chemokine-provoked leukocyte-endothelium interactions have remained elusive. Numerous studies have shown that leukocyte recruitment is a rate-limiting step in acute infl ammation [24][25][26].…”

Therapeutic Effects of Xanthine Oxidase Inhibitors: Renaissance Half a Century after the Discovery of Allopurinol

“…Xanthine Oxidase and Splanchnic Ischemia-Reperfusion. Allopurinol pretreatment is protective in the ischemic and reperfused gut and beneficially affects the changes in vascular permeability (Parks et al, 1982;Parks and Granger, 1983;Granger et al, 1986;Kulah et al, 2004), neutrophil infiltration (Grisham et al, 1986;Riaz et al, 2002), bacterial translocation (Deitsch et al, 1988;Vaughan et al, 1992), intestinal inflammatory chemokine levels (Riaz et al, 2003), motility (Hakguder et al, 2002), and mortality (Megison et al, 1990). Most of the early studies are reviewed in Schoenberg and Beger (1993).…”

Intestinal ischemia/reperfusion: microcirculatory pathology and functional consequences

“…Accordingly, intestinal I/Rinduced leukocyte adhesion in LFA-1 (CD11a/CD18)-deficient mice is found reduced by more than 95% [56]. Besides these specific adhesion molecules, trafficking of leukocytes is regulated by secreted CXC chemokines [59] with both macrophage inflammatory protein-2 and cytokine-induced neutrophils chemoattractant playing a key role in promoting leukocyte-endothelial interactions during post-ischemic reperfusion [60,61]. In a murine model of colonic I/R, CXC chemokine expression has been shown to be regulated by xanthine-oxidase-dependent oxygen free radicals [61] and to be inhibited by blockade of Rho-kinase signaling [62].…”