Oxygen plasma treatment for reducing hydrophobicity of a sealed polydimethylsiloxane microchannel

Tan, Say Hwa; Nguyen, Nam‐Trung; Chua, Yong Chin.; Kang, Tae Goo

doi:10.1063/1.3466882

Cited by 353 publications

(259 citation statements)

References 30 publications

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“…[56][57][58] Given the high water content of hydrogels, their direct contact with PDMS should impart a similar protection of the surface treatment.…”

mentioning

confidence: 99%

3D Printing of Transparent and Conductive Heterogeneous Hydrogel–Elastomer Systems

et al. 2017

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A hydrogel–dielectric‐elastomer system, polyacrylamide and poly(dimethylsiloxane) (PDMS), is adapted for extrusion printing for integrated device fabrication. A lithium‐chloride‐containing hydrogel printing ink is developed and printed onto treated PDMS with no visible signs of delamination and geometrically scaling resistance under moderate uniaxial tension and fatigue. A variety of designs are demonstrated, including a resistive strain gauge and an ionic cable.

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“…[56][57][58] Given the high water content of hydrogels, their direct contact with PDMS should impart a similar protection of the surface treatment.…”

mentioning

confidence: 99%

3D Printing of Transparent and Conductive Heterogeneous Hydrogel–Elastomer Systems

et al. 2017

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“…A 10:1 PDMS (polydimethylsiloxane) and curing agent mix was cast onto the masters and cured in the oven at 90 °C for 2 h. Microfluidic chips were cut and gently released from the masters, inlet and outlet holes were punched and PDMS replicas were irreversibly bonded on coverslips (Thickness no. 1.5 (0.16 to 0.19 mm), VWR, Stockholm, Sweden) by oxygen plasma treatment (PDC-32G/32G-2 (115/230V), Harrick Plasma, Ithaca, NY, USA) at 18 W RF power for 30 s. To increase the surface wettability and reduce the risk of bubble formation inside the channels, sealed microfluidic devices underwent an extended oxygen plasma treatment step for 5 min before performing the experiments [33]. Detailed fabrication procedure has been described by Sott et al [34].…”

Section: Microfluidic System Design and Fabricationmentioning

confidence: 99%

Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications

et al. 2013

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Abstract:The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 μm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min −1 , moderate = 50 nL min −1 , and high = 100 nL min −1 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types.

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“…2 Fabrication process of the two-layered SU-8 mold: a silicon substrate, b Al thin film deposition using radiofrequency magnetron sputtering, c spin coating and patterning of a 10-lm thick layer of SU-8 for clearance, d development of SU-8, e etching Al thin film for alignment, f spin coating and patterning of a 500-lm thick SU-8 layer, g development of SU-8, h pouring PDMS onto the SU-8 mold, and i punching holes for the inlet and the outlet in PDMS valve and the side walls of the microchannel, and enables the micro-VFP applicable to various working fluids. In addition, a specific surface treatment (Tan et al 2010) can be useful to maintain the hydrophilicity of PDMS surface, which will enable us to use water as a working fluid. The motion of the gas-liquid interface in the reservoirs is observed using a CCD camera (WAT-231S2, Watec Co., Ltd., Japan) and is analyzed by in-house software, which tracks the horizontal gas-liquid interface in real time.…”

Section: Methodsmentioning

confidence: 99%

Development of micro-vibrating flow pumps using MEMS technologies

Osman

Shintaku

2012

Microfluid Nanofluid

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In the present paper, we propose a microvibrating flow pump (micro-VFP), which is a novel micropump. The micro-VFP is constructed using an actively vibrating valve that has a cantilever-like structure fixed on a wall of a microchannel and a slit orifice downstream. The slit orifice is designed to make the flow asymmetric around the vibrating valve and to effectively generate a net flow in one direction. At the same time, the valve works as an actuator to induce liquid flow in the microchannel. Since the valve is made of a flexible material including magnetic particles, it is manipulated by changing the magnetic field from outside the micro-VFP. This design allows external operation of the micro-VFP without any electrical or mechanical connections. In addition, the micro-VFP, which realizes pumping with a chamber free design, is advantageous for implementation in a small space. In order to demonstrate its basic pumping performance, a prototype micro-VFP was fabricated in a microchannel with a cross section of 240 lm 9 500 lm using microelectromechanical systems technologies. The vibration characteristics of the valve were investigated using a high-speed camera. The pump performance at various actuation frequencies in the range of 5 to 25 Hz was evaluated by measuring the hydrostatic head and the flow rate. The proposed micro-VFP design exhibited an increase in performance with the driving frequency and had a maximum shut-off pressure of 3.8 ± 0.4 Pa and a maximum flow rate of 0.38 ± 0.02 ll/min at 25 Hz. Furthermore, in order to clarify the detailed pumping process, the flow characteristics around the vibrating valve were investigated by analyzing the velocity field based on micron-resolution particle image velocimetry (micro-PIV). The validity of the hydrostatic measurement was confirmed by comparing the volume flow rate with that estimated from micro-PIV data. The present study revealed the basic performance of the developed micro-VFP.

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Oxygen plasma treatment for reducing hydrophobicity of a sealed polydimethylsiloxane microchannel

Cited by 353 publications

References 30 publications

3D Printing of Transparent and Conductive Heterogeneous Hydrogel–Elastomer Systems

3D Printing of Transparent and Conductive Heterogeneous Hydrogel–Elastomer Systems

Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications

Development of micro-vibrating flow pumps using MEMS technologies

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