Oxygen metabolism in plant cell culture/bacteria interactions: role of bacterial concentration and H2O2-scavenging in survival under biological and artificial oxidative stress

Baker, C. Jacyn; Orlandi, Elizabeth W.; Anderson, Anne J.

doi:10.1006/pmpp.1997.0132

Cited by 14 publications

(6 citation statements)

References 23 publications

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“…In particular, when plant cells come into contact with bacteria, the plant cells release an immediate (and non-bacterial strain-specific) burst of H 2 O 2 (26). In tobacco cell suspensions, this H 2 O 2 burst typically falls below the 100 M range, and Baker et al (27) demonstrate that catalase, because of its high K m (anywhere from 47 to 1100 mM), is inefficient at scavenging H 2 O 2 at such concentrations. Indeed, wild type P. putida were no better able to survive a range of H 2 O 2 concentration from 0.001 to 1 mM than an isogenic catalase-deficient mutant (27).…”

Section: Fig 2 H 2 O 2 -Scavenging (Open Symbols) and Growth (As Mementioning

confidence: 99%

“…In tobacco cell suspensions, this H 2 O 2 burst typically falls below the 100 M range, and Baker et al (27) demonstrate that catalase, because of its high K m (anywhere from 47 to 1100 mM), is inefficient at scavenging H 2 O 2 at such concentrations. Indeed, wild type P. putida were no better able to survive a range of H 2 O 2 concentration from 0.001 to 1 mM than an isogenic catalase-deficient mutant (27). This observation highlights a need for one or more alternative mechanisms for H 2 O 2 scavenging in P. putida, and we propose that quinone-mediated quenching by ChrR is likely to be particularly relevant at M levels of H 2 O 2 .…”

Section: Fig 2 H 2 O 2 -Scavenging (Open Symbols) and Growth (As Mementioning

confidence: 99%

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ChrR, a Soluble Quinone Reductase of Pseudomonas putida That Defends against H2O2

González¹,

Ackerley²,

Lynch

et al. 2005

Journal of Biological Chemistry

131

119

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Most bacteria contain soluble quinone-reducing flavoenzymes. However, no biological benefit for this activity has previously been demonstrated. ChrR of Pseudomonas putida is one such enzyme that has also been characterized as a chromate reductase; yet we propose that it is the quinone-reducing activity of ChrR that has the greatest biological significance. ChrR reduces quinones by simultaneous two-electron transfer, avoiding formation of highly reactive semiquinone intermediates and producing quinols that promote tolerance of H 2 O 2 . Expression of chrR was induced by H 2 O 2 , and levels of chrR expression in overexpressing, wild type, and knock-out mutant strains correlated with the H 2 O 2 tolerance and scavenging ability of each strain. The chrR expression level also correlated with intracellular H 2 O 2 levels as measured by protein carbonylation assays and fluorescence-activated cell scanning analysis with the H 2 O 2 -responsive dye H 2 DCFDA. Thus, enhancing the activity of ChrR in a chromate-remediating bacterial strain may not only increase the rate of chromate transformation, it may also augment the capacity of these cells to withstand the unavoidable production of H 2 O 2 that accompanies chromate reduction.

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Section: Fig 2 H 2 O 2 -Scavenging (Open Symbols) and Growth (As Mementioning

confidence: 99%

Section: Fig 2 H 2 O 2 -Scavenging (Open Symbols) and Growth (As Mementioning

confidence: 99%

ChrR, a Soluble Quinone Reductase of Pseudomonas putida That Defends against H2O2

González¹,

Ackerley²,

Lynch

et al. 2005

Journal of Biological Chemistry

131

119

View full text Add to dashboard Cite

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“…Six of the catalase‐negative isolates grew aerobically in PYGV and two in glucose media, indicating that they may possess alternative methods to destroy H 2 O 2 as has been proposed for plant‐associated Pseudomonas spp. [35].…”

Section: Discussionmentioning

confidence: 99%

Psychrotolerant and microaerophilic bacteria in boreal groundwater

Männistö

Puhakka

2002

FEMS Microbiology Ecology

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Growth temperature and microaerophily of 39 phylogenetically different isolates from boreal oxygen-deficient groundwater were studied. Based on growth temperatures, the isolates were mainly psychrotolerant bacteria as 35 grew at 2 degrees C and only three at 35 degrees C. Growth rates in the range of 4-35 degrees C fitted the Ratkowsky square root model well. Optimum temperatures of the groundwater isolates varied between 18 and 30 degrees C. In semisolid glucose and PYGV media, 59% and 28% of the isolates, respectively, preferred microaerophilic growth and 33% were catalase-negative. The microaerophilic isolates had the highest sensitivity to H(2)O(2) whereas sensitivity to the superoxide generator paraquat was similar among microaerophilic and aerobic isolates. The results show that the cold (6-8 degrees C) and oxygen-deficient groundwater harbors psychrotolerant and microaerophilic bacteria of different phylogenetic origins which are well adapted to their environment.

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“…This difference could contribute to the ability of P. putida isolates to continue increasing oxygen uptake at lower concentrations of oxygen. Unlike the plant oxygen uptake response, there was no concurrent measurable H 2 0 2 accumulation by the bacteria, even when lower bacterial concentrations were used to avoid H202-scavenging [2]. I t is still feasible and probable that this increased oxygen uptake was leading to increased superoxide and hydrogen peroxide [ 4 , 9, 131 production, however, it may not accumulate to allou measurement.…”

Section: Discussionmentioning

confidence: 99%

Oxidative metabolism in plant/bacteria interactions: characterization of the oxygen uptake response of bacteria

Baker

Mock

Deahl

et al. 2001

Physiological and Molecular Plant Pathology

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An increase in oxygen uptake has been previously described in plant cell suspensions treated with bacteria or bacterial elicitors. These studies, regarding oxygen uptake, have all been undertaken from the perspective of the host plant cell reactirlg to the invading pathogen. In contrast, here we describe and characterize an increase in oxygen uptake by bacterial cells in response to plant suspensions or autoclaved plant cell filtrates. Autoclaved plant cell filtrates stimulated bacterial oxygen uptake by as much as sevenfold within a few minutes after addition. This oxygen uptake response was proportiorlal to both the concentration of the plant cell filtrate and the concentration of the bacteria. KCN inhibited the bacterial response, suggesting that bacterial respiratiorl may be involved. Unlike the plant oxygen uptake response to bacteria, there was no concurrent H 2 0 2 accumulation and the NADPH oxidase inhibitor, DPI, had no effect on the bacterial response. Streptomycin, an inhibitor of bacterial protein synthesis, irlllibited the bacterial oxygen uptake response to the plant cell filtrate. K-252, a protein kinase inhibitor that strongly irlhibits the plant oxygen uptake response to bacteria, had no eEect upor1 the hacterial oxygen uptake response. IVhen potatolbacterial cell suspensions were pretreated with either streptomycin or K-252, the combined plantlbacterial oxygen uptake response was inhibited by 15 or 70 %, respectively. This indicates that as much as 15-30 % of the increased oxygen consumption during plant suspension cell/bacteria interactions may be attributable to bacteria, which comprise less than 1 % of the total cell mass.

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Oxygen metabolism in plant cell culture/bacteria interactions: role of bacterial concentration and H2O2-scavenging in survival under biological and artificial oxidative stress

Cited by 14 publications

References 23 publications

ChrR, a Soluble Quinone Reductase of Pseudomonas putida That Defends against H2O2

ChrR, a Soluble Quinone Reductase of Pseudomonas putida That Defends against H2O2

Psychrotolerant and microaerophilic bacteria in boreal groundwater

Oxidative metabolism in plant/bacteria interactions: characterization of the oxygen uptake response of bacteria

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