Oxygen limitations and aging as explanations for the field persistence of naphthalene in coal tar‐contaminated surface sediments

Madsen, Eugene L.; Mann, Cynthia L.; Bilotta, Sharon E.

doi:10.1002/etc.5620151104

Cited by 38 publications

(34 citation statements)

References 31 publications

(38 reference statements)

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“…6, 20, and 21). To discover the identity of the active populations, we implemented field respiration and SIP procedures (19), which released 13 C-labeled naphthalene where flowing groundwater has deposited naphthalene in organic matter-rich surface sediment (22). The small volumes of naphthalene-dosed sediment (Ϸ50 ml) were covered by open-bottom glass chambers that allowed headspace gases produced by the native microbial community to be periodically gathered and analyzed for evolution of 13 CO 2 above background produced from sediment organic matter (19).…”

Section: Resultsmentioning

confidence: 99%

“…If a high-substrate concentration had been used, SIP may have revealed opportunistic microorganisms other than those active under ambient site conditions. Because the mass of 13 C-naphthalene added (Ϸ60 g͞5 g sediment) roughly matched ambient concentrations (22), we feel that potential concentration-based artifacts were avoided. Data interpretation presumed that the 16S rRNA gene sequences found in 13 C-labeled DNA were indicative of microbial populations directly responsible for naphthalene biodegradation.…”

Section: Discussionmentioning

confidence: 98%

“…The site is a shallow unconfined aquifer (water table at Ϸ2.5 m depth) near the western edge of the Hudson River. Coal-tar waste, rich in naphthalene and other aromatic hydrocarbons, was deposited 40 years ago in this flat, sandy, forested area where flowing groundwater has created a narrow subsurface plume of coal-tar constituents and has deposited naphthalene in surface sediments in a low-lying seepage area (6,(19)(20)(21)(22).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Discovery of a bacterium, with distinctive dioxygenase, that is responsible for in situ biodegradation in contaminated sediment

Jeon

Park

Parameswaran

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

226

173

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Microorganisms maintain the biosphere by catalyzing biogeochemical processes, including biodegradation of organic chemical pollutants. Yet seldom have the responsible agents and their respective genes been identified. Here we used field-based stable isotopic probing (SIP) to discover a group of bacteria responsible for in situ metabolism of an environmental pollutant, naphthalene. We released 13 C-labeled naphthalene in a contaminated study site to trace the flow of pollutant carbon into the naturally occurring microbial community. Using GC͞MS, molecular biology, and classical microbiological techniques we documented 13 CO2 evolution (2.3% of the dose in 8 h), created a library of 16S rRNA gene clones from 13 C labeled sediment DNA, identified a taxonomic cluster (92 of 95 clones) from the microbial community involved in metabolism of the added naphthalene, and isolated a previously undescribed bacterium (strain CJ2) from site sediment whose 16S rRNA gene matched that of the dominant member (48%) of the clone library. Strain CJ2 is a ␤ proteobacterium closely related to Polaromonas vacuolata. Moreover, strain CJ2 hosts the sequence of a naphthalene dioxygenase gene, prevalent in site sediment, detected before only in environmental DNA. This investigative strategy may have general application for elucidating the bases of many biogeochemical processes, hence for advancing knowledge and management of ecological and industrial systems that rely on microorganisms.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Discovery of a bacterium, with distinctive dioxygenase, that is responsible for in situ biodegradation in contaminated sediment

Jeon

Park

Parameswaran

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

226

173

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“…Chemotaxis has been proposed to have an impact on the fate of contaminants in the environment (Pandey and Jain, 2002;Parales and Harwood, 2002), but the availability of dissolved oxygen can be limited at contaminated sites (Madsen et al, 1996). Here, we have shown that low dissolved oxygen concentrations and aerotaxis can inhibit chemotaxis to naphthalene and, consequently, the rate of naphthalene degradation under aerobic conditions.…”

Section: Discussionmentioning

confidence: 86%

The effect of oxygen on chemotaxis to naphthalene by Pseudomonas putida G7

Law

Aitken

2006

Biotech & Bioengineering

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Chemotactic bacteria can be attracted to electron donors they consume. In systems where donor is heterogeneously distributed, chemotaxis can lead to enhanced removal of donor relative to that achieved in the absence of chemotaxis. However, simultaneous consumption of an electron acceptor may result in the formation of an acceptor gradient to which the bacteria also respond, thus diminishing the positive effect of chemotaxis. Depletion of an electron acceptor can also reduce the rate of electron donor consumption in addition to its effect on chemotaxis. In this study, we examined the effect of oxygen on chemotaxis to naphthalene and on naphthalene consumption by Pseudomonas putida G7. The organism was able to move up an oxygen gradient when there was a naphthalene gradient in the opposite direction. In the absence of an oxygen gradient, low levels of oxygen attenuated chemotaxis to naphthalene but did not affect random motility. The rate of naphthalene consumption decreased at dissolved oxygen concentrations similar to those at which chemotaxis was attenuated. These results suggest that low dissolved oxygen concentrations can reduce naphthalene removal by P. putida G7 in systems where naphthalene is heterogeneously distributed by simultaneously attenuating chemotactic motion toward naphthalene and decreasing the rate of naphthalene degradation.

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“…Characteristics and details of this site have been previously published (24)(25)(26)(27)42). Naphthalene-degrading strains isolated from contaminated sediment from our study site (designated Cg) have been previously described (19).…”

mentioning

confidence: 99%

Naphthalene and Donor Cell Density Influence Field Conjugation of Naphthalene Catabolism Plasmids

Hohnstock

Stuart-Keil

Kull

et al. 2000

Appl Environ Microbiol

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We examined transfer of naphthalene-catabolic genes from donor microorganisms native to a contaminated site to site-derived, rifampin-resistant recipient bacteria unable to grow on naphthalene. Horizontal gene transfer (HGT) was demonstrated in filter matings using groundwater microorganisms as donors. Two distinct but similar plasmid types, closely related to pDTG1, were retrieved. In laboratory-incubated sediment matings, the addition of naphthalene stimulated HGT. However, recipient bacteria deployed in recoverable vessels in the field site (in situ) did not retrieve plasmids from native donors. Only when plasmid-containing donor cells and naphthalene were added to the in situ mating experiments did HGT occur.

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Oxygen limitations and aging as explanations for the field persistence of naphthalene in coal tar‐contaminated surface sediments

Cited by 38 publications

References 31 publications

Discovery of a bacterium, with distinctive dioxygenase, that is responsible for in situ biodegradation in contaminated sediment

Discovery of a bacterium, with distinctive dioxygenase, that is responsible for in situ biodegradation in contaminated sediment

The effect of oxygen on chemotaxis to naphthalene by Pseudomonas putida G7

Naphthalene and Donor Cell Density Influence Field Conjugation of Naphthalene Catabolism Plasmids

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