Oxygen generating biomaterial improves the function and efficacy of beta cells within a macroencapsulation device

Coronel, María M.; Liang, Jia-Pu; Li, Y.; Stabler, Cherie L.

doi:10.1016/j.biomaterials.2019.04.017

Cited by 62 publications

(42 citation statements)

References 62 publications

(99 reference statements)

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“…Differentiated hESCs offer a potentially unlimited number of cells for this purpose [19], and this approach is enhanced by improving the maturation of the generated SC-β cells [20]. The inclusion of endothelial cells with SC-β cells could be combined with macroporous scaffolds that enable retrievability [16,21] or other beneficial materials [22][23][24][25][26] to develop a more comprehensive transplantation strategy for diabetes. An alternative cell type to SC-β cells are earlier pancreatic progenitors derived from hESCs.…”

Section: Discussionmentioning

confidence: 99%

Hydrogel platform for in vitro three-dimensional assembly of human stem cell-derived β cells and endothelial cells

Augsornworawat¹,

Velazco-Cruz²,

Song³

et al. 2019

Preprint

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Differentiation of stem cells into functional replacement cells and tissues is a major goal of the regenerative medicine field. However, one limitation has been organization of differentiated cells into multi-cellular, three-dimensional assemblies. The islets of Langerhans contain many endocrine and non-endocrine cell types, such as insulin-producing β cells and endothelial cells. Transplantation of exogenous islets into diabetic patients can serve as a cell replacement therapy, replacing the need for patients to inject themselves with insulin, but the number of available islets from cadaveric donors is low. We have developed a strategy of assembling human embryonic stem cell-derived β cells with endothelial cells into three-dimensional aggregates on a hydrogel. The resulting islet organoids express β cell markers and are functional, capable of undergoing glucose-stimulated insulin secretion. These results provide a platform for evaluating the effects of the islet tissue microenvironment on human embryonic stem cell-derived β cells and other islet endocrine cells to develop tissue engineered islets.

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Section: Discussionmentioning

confidence: 99%

Hydrogel platform for in vitro three-dimensional assembly of human stem cell-derived β cells and endothelial cells

Augsornworawat¹,

Velazco-Cruz²,

Song³

et al. 2019

Preprint

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“…PDMS is a widely used material in biomedical applications such as implants, devices, and cellular studies because of its nontoxicity and biocompatibility properties. [33][34][35][36][37] PDMS possesses favorable unique properties as it is permeable to gases, chemically inert, thermally stable, rigid, and optically clear. [38][39][40][41] Lai and Liu 32 and Huang et al 30 have fabricated cubic eggshells made of PDMS with an internal egg-shaped feature in the former model but not in the latter.…”

Section: Glass Bowl Glass Bowl With Clear Lid Angiogenesis and Vasculmentioning

confidence: 99%

“…[33][34][35][36][37] PDMS possesses favorable unique properties as it is permeable to gases, chemically inert, thermally stable, rigid, and optically clear. More recently, polydimethylsiloxane (PDMS) has been utilized to form the culture vessels.…”

mentioning

confidence: 99%

Bioengineered three‐dimensional transparent eggshell as a chicken embryo experimentation platform for biomedical research

Ishak

Chan

Ang

et al. 2020

Engineering Reports

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The chicken embryo is widely used as an experimental model in the areas of regenerative medicine, tumor biology, and angiogenesis. Eggshell opacity and rigidity present restricted three-dimensional (3D) viewing and accessibility to the embryo and its circulatory network despite egg windowing. The ability to engineer an eggshell, which eliminates the opacity yet provides 3D access for manipulation is beneficial and would potentially make it enticing for the use of chicken embryo as a cheaper alternative vertebrate model.Here, we present the feasibility of fabricating a morphologically analogous transparent chicken eggshell made of polydimethylsiloxane using a biaxial rotation computer-controlled bioreactor to achieve uniform thickness. By culturing chicken embryo in the bioengineered eggshell, we demonstrated success in meeting developmental milestones and its practicality as an experimental platform by visualizing both embryo and vasculature development using common laboratory imaging tools. Although the viability of the embryo in the bioengineered eggshell was lower than in the normal egg, this was attributed to the absence of calcium source. The bioengineered eggshell, in its initial stage of development, provides a platform to be used to investigate beyond gross observations of the embryo and vascular network in 3D optical clarity and its imaging capabilities has the potential to be extended to other imaging modalities. We have developed a transparent ex ovo eggshell, biomimicking the elliptical geometry to address the lack of 3D optical clarity contributed by the eggshell opacity.This retains the natural shape that may support the development of the chicken embryo and allows easy access to the embryo, its circulatory system and respective organ development in far reaching applications. K E Y W O R D Sbioengineering, chicken embryo, fabrication, imaging, rotation moldingThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.The use of chicken embryo as an experimental model confers several advantages over other vertebrate models. It is a unique model, which allows relatively easy access to the embryo and its circulatory system, rendering the possibility for embryo manipulation and monitoring of the chicken embryo development from blastoderm formation until hatching. The chicken embryo is an inexpensive model with a relatively short embryonic development of 21 days. 1,2 In addition, they are ethically acceptable, only requiring animal ethics approval if hatching is expected. Moreover, its amenability to xenografting due to its naturally immunodeficient system in the first 2 weeks of development allows cell transplantation and cell tracking without rejection, rendering it an attractive model widely used in tumor biology, angiogenesis, and regenerative medicine studies. 3-11 These embryo manipulations and visualizati...

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“…Solid calcium peroxide encapsulated within PDMS discs provides controllable generation of oxygen, and these discs (termed OxySite) have been used for long-term, stable generation of oxygen for islets in in vitro culture and in vivo macroencapsulation. [250][251][252] Manipulating the nanotopographical environment can regulate cellular functions and direct stem cell fate. [253,254] Gradient nanopatterned chips composed of polystyrene recently enabled the identification of a pore nanotopography (200-300 nm diameter) that promoted the aggregation and efficient differentiation of β-cell organoids from hPSCs.…”

Section: Other Synthetic Biomaterialsmentioning

confidence: 99%

“…Solid calcium peroxide encapsulated within PDMS discs provides controllable generation of oxygen, and these discs (termed OxySite) have been used for long‐term, stable generation of oxygen for islets in in vitro culture and in vivo macroencapsulation. [ 250–252 ]…”

Section: Engineered Biomaterials For β‐Cell Organoidsmentioning

confidence: 99%

Engineered Biomaterials for Enhanced Function of Insulin‐Secreting β‐Cell Organoids

Hunckler

Garcı́a

2020

Adv Funct Materials

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Insulin-secreting β-cell organoids comprise many types of cells, including primary islets, pseudoislets, immortalized cell lines, and stem cell-derived aggregates. Successful maintenance and long-term culture of these β-cell organoids are critical for many translational applications, such as patient-specific disease modeling, drug screening, understanding β-cell physiology, and cell-based therapies to treat diabetes. Due to the mechanical and chemical insult to islets during isolation, islet viability and function are decreased. Partial restoration of the islet microenvironment through engineered biomaterials can improve islet survival and function in in vitro culture and in vivo transplantation. Natural and synthetic biomaterials can be engineered to improve the function of β-cell organoids and promote maturation of stem cell-derived β-cell organoids. Improved function and maturation of β-cell organoids will likely lead to improved transplant outcomes, but will also enable better models for physiology, disease modeling, and toxicology studies for type 1 and type 2 diabetes mellitus. The goal of this review is to highlight the diverse array of biomaterials used to enhance the in vitro and in vivo function and maturation of β-cell organoids.

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Oxygen generating biomaterial improves the function and efficacy of beta cells within a macroencapsulation device

Cited by 62 publications

References 62 publications

Hydrogel platform for in vitro three-dimensional assembly of human stem cell-derived β cells and endothelial cells

Hydrogel platform for in vitro three-dimensional assembly of human stem cell-derived β cells and endothelial cells

Bioengineered three‐dimensional transparent eggshell as a chicken embryo experimentation platform for biomedical research

Engineered Biomaterials for Enhanced Function of Insulin‐Secreting β‐Cell Organoids

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