Oxygen dependency of germinating Brassica seeds

Park, Myoung Ryoul; Hasenstein, Karl H.

doi:10.1016/j.lssr.2016.01.002

Cited by 16 publications

(12 citation statements)

References 61 publications

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“…Aerobic respiration provides the major supply of the energy required for germination, making oxygen supply vital (Park & Hasenstein, 2016). In Arabidopsis, the reactivation of mitochondria is immediate upon rehydration (Paszkiewicz et al, 2017).…”

Section: Respiratory Oxygen Consumption Is Initiated First In the Endmentioning

confidence: 99%

A functional imaging study of germinating oilseed rape seed

et al. 2017

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Germination, the process whereby a dry, quiescent seed springs to life, has been a focus of plant biologist for many years, yet the early events following water uptake, during which metabolism of the embryo is restarted, remain enigmatic. Here, the nature of the cues required for this restarting in oilseed rape (Brassica napus) seed has been investigated. A holistic in vivo approach was designed to display the link between the entry and allocation of water, metabolic events and structural changes occurring during germination. For this, we combined functional magnetic resonance imaging with Fourier transform infrared microscopy, fluorescence-based respiration mapping, computer-aided seed modeling and biochemical tools. We uncovered an endospermal lipid gap, which channels water to the radicle tip, from whence it is distributed via embryonic vasculature toward cotyledon tissues. The resumption of respiration is initiated first in the endosperm, only later spreading to the embryo. Sugar metabolism and lipid utilization are linked to the spatiotemporal sequence of tissue rehydration. Together, this imaging study provides insights into the spatial aspects of key events in oilseed rape seeds leading to germination. It demonstrates how seed architecture predetermines the pattern of water intake, which sets the stage for the orchestrated restart of life.

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Section: Respiratory Oxygen Consumption Is Initiated First In the Endmentioning

confidence: 99%

A functional imaging study of germinating oilseed rape seed

et al. 2017

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“…Seed germination is regulated by various hormones and environmental factors, where adequate oxygen supply is also important before the onset of photosynthetic activity. Flooding during the germination stage is believed to limit mitochondrial respiration, energy metabolism, and the gas diffusion between cells [ 7 ]. Restricted aerobic respiration under flooding conditions also leads to outbursts of reactive oxygen species (ROS), which in turn causes reprogramming of gene expression at cellular and molecular levels [ 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals Genes of Flooding-Tolerant and Flooding-Sensitive Rapeseeds Differentially Respond to Flooding at the Germination Stage

Iqbal

Zhang

et al. 2021

Plants

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Flooding results in significant crop yield losses due to exposure of plants to hypoxic stress. Various studies have reported the effect of flooding stress at seedling establishment or later stages. However, the molecular mechanism prevailing at the germination stage under flooding stress remains enigmatic. The present study highlights the comparative transcriptome analysis in two rapeseed lines, i.e., flooding-tolerant (Santana) and -sensitive (23651) lines under control and 6-h flooding treatments at the germination stage. A total of 1840 up-regulated and 1301 down-regulated genes were shared by both lines in response to flooding. There were 4410 differentially expressed genes (DEGs) with increased expression and 4271 DEGs with reduced expression shared in both control and flooding conditions. Gene ontology (GO) enrichment analysis revealed that “transcription regulation”, “structural constituent of cell wall”, “reactive oxygen species metabolic”, “peroxidase”, oxidoreductase”, and “antioxidant activity” were the common processes in rapeseed flooding response. In addition, the processes such as “hormone-mediated signaling pathway”, “response to organic substance response”, “motor activity”, and “microtubule-based process” are likely to confer rapeseed flooding resistance. Mclust analysis clustered DEGs into nine modules; genes in each module shared similar expression patterns and many of these genes overlapped with the top 20 DEGs in some groups. This work provides a comprehensive insight into gene responses and the regulatory network in rapeseed flooding stress and provides guidelines for probing the underlying molecular mechanisms in flooding resistance.

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“…Other enzymes identified that were previously reported in seeds were UDP-Glycosyl transferase (an activity involved in the glycosylation of different metabolites; [39], beta glucosidases, UDP-glucose pyrophosphorylase 2 (whose gene inactivation impeded seed set in rice); [40], UDP-D-apiose/UDP-D-xylose synthase 2 (related to cell wall formation [41]. The set in cotyledons comprises the sugar isomerase (SIS) family protein [42], the UDP-glucose 6-dehydrogenase family protein [43], glucose-1-phosphate adenylyl transferase, sucrose synthase 2 [44], GDP-D-mannose 3 ,5 -epimerase [45]. The evolution in cotyledons was similar to the previous ones, with the maximum at M7-M9 with later a decay/degradation at germination.…”

Section: Discussionmentioning

confidence: 99%

Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy

Sghaier-Hammami

Hammami

Baazaoui

et al. 2020

IJMS

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Unlike orthodox species, seed recalcitrance is poorly understood, especially at the molecular level. In this regard, seed maturation and germination were studied in the non-orthodox Quercus ilex by using a proteomics strategy based on two-dimensional gel electrophoresis coupled to matrix-assisted laser desorption ionization/time of flight (2-DE-MALDI-TOF).Cotyledons and embryo/radicle were sampled at different developmental stages, including early (M1–M3), middle (M4–M7), and late (M8–M9) seed maturation, and early (G1–G3) and late (G4–G5) germination. Samples corresponding to non-germinating, inviable, seeds were also included. Protein extracts were subjected to 2-dimensional gel electrophoresis (2-DE) and changes in the protein profiles were analyzed. Identified variable proteins were grouped according to their function, being the energy, carbohydrate, lipid, and amino acid metabolisms, together with protein fate, redox homeostasis, and response to stress are the most represented groups. Beyond the visual aspect, morphometry, weight, and water content, each stage had a specific protein signature. Clear tendencies for the different protein groups throughout the maturation and germination stages were observed for, respectively, cotyledon and the embryo axis. Proteins related to metabolism, translation, legumins, proteases, proteasome, and those stress related were less abundant in non-germinating seeds, it related to the loss of viability. Cotyledons were enriched with reserve proteins and protein-degrading enzymes, while the embryo axis was enriched with proteins of cell defense and rescue, including heat-shock proteins (HSPs) and antioxidants. The peaks of enzyme proteins occurred at the middle stages (M6–M7) in cotyledons and at late ones (M8–M9) in the embryo axis. Unlike orthodox seeds, proteins associated with glycolysis, tricarboxylic acid cycle, carbohydrate, amino acid and lipid metabolism are present at high levels in the mature seed and were maintained throughout the germination stages. The lack of desiccation tolerance in Q. ilex seeds may be associated with the repression of some genes, late embryogenesis abundant proteins being one of the candidates.

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Oxygen dependency of germinating Brassica seeds

Cited by 16 publications

References 61 publications

A functional imaging study of germinating oilseed rape seed

A functional imaging study of germinating oilseed rape seed

Transcriptome Analysis Reveals Genes of Flooding-Tolerant and Flooding-Sensitive Rapeseeds Differentially Respond to Flooding at the Germination Stage

Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy

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