Oxygen dependence of metabolic fluxes and energy generation of Saccharomyces cerevisiae CEN.PK113-1A

Jouhten, Paula; Rintala, Eija; Huuskonen, Anne; Tamminen, Anu; Toivari, Mervi; Wiebe, Marilyn G.; Ruohonen, Laura; Penttilä, Merja; Maaheimo, Hannu

doi:10.1186/1752-0509-2-60

Cited by 107 publications

(132 citation statements)

References 79 publications

Supporting

Mentioning

118

Contrasting

Order By: Relevance

“…Up till now, metabolic profiling and flux analysis have been established for analyzing intermediate metabolites in ethanol fermentation with Saccharomyces cerevisiae under low and normal concentration substrate conditions (Jouhten et al, 2008;Nissen et al, 1997;Varela et al, 2004), but how metabolic flux distribution is affected under VHC conditions has been rarely exploited. Devantier et al (2005) performed metabolic profiling analysis for batch VHC fermentation, but so far no related study has been reported for continuous VHC ethanol fermentation systems.…”

Section: Introductionmentioning

confidence: 99%

Effect of the size of yeast flocs and zinc supplementation on continuous ethanol fermentation performance and metabolic flux distribution under very high concentration conditions

Xue

Zhao

Bai

2010

Biotech & Bioengineering

View full text Add to dashboard Cite

Taking continuous ethanol fermentation with the self-flocculating yeast SPSC01 under very high concentration conditions as an example, the fermentation performance of the yeast flocs and their metabolic flux distribution were investigated by controlling their average sizes at 100, 200, and 300 mm using the focused beam reflectance online measurement system. In addition, the impact of zinc supplementation was evaluated for the yeast flocs at the size of 300 mm grown in presence or absence of 0.05 g L À1 zinc sulfate. Among the yeast flocs with different sizes, the group with the average size of 300 mm exhibited highest ethanol production (110.0 g L À1 ) and glucose uptake rate (286.69 C mmol L À1 h À1 ), which are in accordance with the increased flux from pyruvate to ethanol and decreased flux to glycerol. And in the meantime, zinc supplementation further increased ethanol production and cell viability comparing with the control. Zinc addition enhanced the carbon fluxes to the biosynthesis of ergosterol (28.6%) and trehalose (43.3%), whereas the fluxes towards glycerol, protein biosynthesis, and tricarboxylic acid cycle significantly decreased by 37.7%, 19.5%, and 27.8%, respectively. This work presents the first report on the regulation of metabolic flux by the size of yeast flocs and zinc supplementation, which provides the potential for developing engineering strategy to optimize the fermentation system.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of the size of yeast flocs and zinc supplementation on continuous ethanol fermentation performance and metabolic flux distribution under very high concentration conditions

Xue

Zhao

Bai

2010

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…The presence of oxygen in the culture medium can shift the central yeast metabolism from fermentative to respiratory (30,32,50,60). However, Saccharomyces cerevisiae shows a fully respiratory metabolism only at low growth rates with low sugar concentrations (20).…”

mentioning

confidence: 99%

Oxygen Response of the Wine Yeast Saccharomyces cerevisiae EC1118 Grown under Carbon-Sufficient, Nitrogen-Limited Enological Conditions

Aceituno

Orellana

Torres

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

Discrete additions of oxygen play a critical role in alcoholic fermentation. However, few studies have quantitated the fate of dissolved oxygen and its impact on wine yeast cell physiology under enological conditions. We simulated the range of dissolved oxygen concentrations that occur after a pump-over during the winemaking process by sparging nitrogen-limited continuous cultures with oxygen-nitrogen gaseous mixtures. When the dissolved oxygen concentration increased from 1.2 to 2.7 M, yeast cells changed from a fully fermentative to a mixed respirofermentative metabolism. This transition is characterized by a switch in the operation of the tricarboxylic acid cycle (TCA) and an activation of NADH shuttling from the cytosol to mitochondria. Nevertheless, fermentative ethanol production remained the major cytosolic NADH sink under all oxygen conditions, suggesting that the limitation of mitochondrial NADH reoxidation is the major cause of the Crabtree effect. This is reinforced by the induction of several key respiratory genes by oxygen, despite the high sugar concentration, indicating that oxygen overrides glucose repression. Genes associated with other processes, such as proline uptake, cell wall remodeling, and oxidative stress, were also significantly affected by oxygen. The results of this study indicate that respiration is responsible for a substantial part of the oxygen response in yeast cells during alcoholic fermentation. This information will facilitate the development of temporal oxygen addition strategies to optimize yeast performance in industrial fermentations.

show abstract

“…In S. cerevisiae, ethanol biosynthesis reactions contribute mainly to the oxidization of NADH produced through glycolysis to maintain the intracellular balance of NADH and NAD + . 52) Moreover, when the ethanol biosynthesis pathways are disrupted, glycerol biosynthesis is induced to oxidize NADH produced through glycolysis. 53,54) Hence, in our experiments, glycerol production was increased in ADH1-5-disrupted strains S149 and S149sdh12.…”

Section: Resultsmentioning

confidence: 99%

Metabolic engineering of Saccharomyces cerevisiae to improve succinic acid production based on metabolic profiling

Ito

Hirasawa

Shimizu

2014

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

We performed metabolic engineering on the budding yeast Saccharomyces cerevisiae for enhanced production of succinic acid. Aerobic succinic acid production in S. cerevisiae was achieved by disrupting the SDH1 and SDH2 genes, which encode the catalytic subunits of succinic acid dehydrogenase. Increased succinic acid production was achieved by eliminating the ethanol biosynthesis pathways. Metabolic profiling analysis revealed that succinic acid accumulated intracellularly following disruption of the SDH1 and SDH2 genes, which suggests that enhancing the export of intracellular succinic acid outside of cells increases succinic acid production in S. cerevisiae. The mae1 gene encoding the Schizosaccharomyces pombe malic acid transporter was introduced into S. cerevisiae, and as a result, succinic acid production was successfully improved. Metabolic profiling analysis is useful in producing chemicals for metabolic engineering of microorganisms.

show abstract

Oxygen dependence of metabolic fluxes and energy generation of Saccharomyces cerevisiae CEN.PK113-1A

Cited by 107 publications

References 79 publications

Effect of the size of yeast flocs and zinc supplementation on continuous ethanol fermentation performance and metabolic flux distribution under very high concentration conditions

Effect of the size of yeast flocs and zinc supplementation on continuous ethanol fermentation performance and metabolic flux distribution under very high concentration conditions

Oxygen Response of the Wine Yeast Saccharomyces cerevisiae EC1118 Grown under Carbon-Sufficient, Nitrogen-Limited Enological Conditions

Metabolic engineering of Saccharomyces cerevisiae to improve succinic acid production based on metabolic profiling

Contact Info

Product

Resources

About