Oxygen consumption of human peripheral blood mononuclear cells in severe human sepsis *

Differential expression of the nuclear-encoded mitochondrial transcriptome in pediatric septic shock

“…In particular, the prominence of NADH dehydrogenase activity in the top gene networks is consistent with decreased complex I gene expression [56] and activity noted in skeletal muscle in human adult sepsis [57,58]. Prior studies using blood samples from patients with sepsis have also demonstrated altered respiratory chain activity in peripheral blood mononuclear cells and platelets [9,11,13,59,60].…”

“…Circulating blood cells from critically ill patients with septic shock exhibit decreased oxidative respiration, electron chain complex activity, mitochondrial turnover, and mitochondrial membrane potential [8][9][10][11][12]. Blood is an easily accessible tissue that can be used to directly measure mitochondrial dysfunction in immune cells and may reflect a systemic process affecting other vital organs.…”

“…Blood is an easily accessible tissue that can be used to directly measure mitochondrial dysfunction in immune cells and may reflect a systemic process affecting other vital organs. Mitochondrial dysfunction in blood cells has been associated with severity of illness, organ dysfunction, mortality, and immunoparalysis in human sepsis [8][9][10][11][12], including children [13]. Differential expression of mitochondrial genes in blood cells has been reported for several diseases in which bioenergetic failure is a postulated mechanism [14][15][16], and injection of endotoxin has been shown to cause widespread suppression of genes encoding for mitochondrial ATP production and protein synthesis within human leukocytes [17].…”

Mitochondria Are Gate-keepers of T Cell Function by Producing the ATP That Drives Purinergic Signaling

“…We found that mitochondrial activity in lymphocytes of septic patients is impaired, which may be an underlying cause of T cell suppression in these patients. This notion is supported by findings that oxygen consumption of peripheral blood mononuclear cells is lower in sepsis patients than in patients without sepsis (48,49) and that the cellular ATP content of circulating cells is lower and lactate levels are higher in sepsis patients compared with patients without sepsis (50).…”

New Prognostic Biomarkers of Mitochondrial Oxidative Stress in Septic Patients

“…The function of the OXPHOS system in septic patients has been analyzed only in studies with small sample sizes (fewer than 40 septic patients and 40 control subjects), and the results are demonstrably inconsistent (67)(68)(69)(70)(71)(72)(73)(74)(75)(76)(77)(78)(79)(80)(81)(82). Some studies have found lower activity of different mitochondrial respiratory complexes in septic patients than in control subjects in muscle biopsies (ie, biceps brachii, triceps brachii, vastus lateralis, serratus anterior), peripheral blood mononuclear cells, and circulating platelets.…”