Oxygen consumption kinetics of Nitrosomonas europaea and Nitrobacter hamburgensis grown in mixed continuous cultures at different oxygen concentrations

Laanbroek, Hendrikus J.; Bodelier, Paul L. E.; Gerards, Saskia

doi:10.1007/s002030050036

Cited by 36 publications

(43 citation statements)

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“…Using whole cells from isolates to calculate oxidation kinetics is a well-established method, and thus our calculated K m values are comparable to previous studies with data from pure cultures (Both et al 1992, Laanbroek et al 1994 Nowka et al 2015, our Figs. 1 & 3).…”

Section: Nitrite Oxidation Kineticssupporting

confidence: 89%

“…3). Isolates from soils, like Nitrobacter hamburgensis and Nitrobacter winogradskyi, have significantly higher K m values of up to 1370 μM (Both et al 1992, Laanbroek et al 1994 trobacter and Nitrococcus indicate low substrate affinity, which could be due to low affinities of the nitrite transporter proteins that shuttle nitrite across the cytoplasmic membrane (Nowka et al 2015). Overall, our results are somewhat ambiguous.…”

Section: Nitrite Oxidation Kineticsmentioning

confidence: 60%

“…So far, investigations on nitrite oxidation kinetics have mainly been based on non-marine pure cultures, enrichment cultures, or natural assembles (Prosser 1989, Both et al 1992, Laanbroek et al 1994, Schramm et al 1999, Blackburne et al 2007, Nowka et al 2015. The aim of this study was to investigate the substrate affinity of marine NOB to link it to environmental conditions and nitrite oxidation kinetics, which thus far has been difficult because nitrite affinity of marine NOB had not yet been investigated in pure cultures.…”

Section: Nitrite Oxidation Kineticsmentioning

confidence: 99%

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Oxidation kinetics and inverse isotope effect of marine nitrite-oxidizing isolates

Jacob¹,

Nowka²,

Merten³

et al. 2017

Aquat. Microb. Ecol.

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Nitrification, the step-wise oxidation of ammonium to nitrite and nitrate, is important in the marine environment because it produces nitrate, the most abundant marine dissolved inorganic nitrogen (DIN) component and N-source for phytoplankton and microbes. This study focused on the second step of nitrification, which is carried out by a distinct group of organisms, nitrite-oxidizing bacteria (NOB). The growth of NOB is characterized by nitrite oxidation kinetics, which we investigated for 4 pure cultures of marine NOB (Nitrospina watsonii 347, Nitrospira sp. Ecomares 2.1, Nitrococcus mobilis 231, and Nitrobacter sp. 311). We further compared the kinetics to those of non-marine species because substrate concentrations in marine environments are comparatively low, which likely influences kinetics and highlights the importance of this study. We also determined the isotope effect during nitrite oxidation of a pure culture of Nitrospina (Nitrospina watsonii 347) belonging to one of the most abundant marine NOB genera, and for a Nitrospira strain (Nitrospira sp. Ecomares 2.1). The enzyme kinetics of nitrite oxidation, described by Michaelis-Menten kinetics, of 4 marine genera are rather narrow and fall in the low end of halfsaturation constant (K m ) values reported so far, which span over 3 orders of magnitude between 9 and >1000 μM NO 2 − . Nitrospina has the lowest K m (19 μM NO 2 − ), followed by Nitrobacter (28 μM NO 2 − ), Nitrospira (54 μM NO 2 − ), and Nitrococcus (120 μM NO 2 − ). The isotope effects during nitrite oxidation by Nitrospina watsonii 347 and Nitrospira sp. Ecomares 2.1 were 9.7 ± 0.8 and 10.2 ± 0.9 ‰, respectively. This confirms the inverse isotope effect of NOB described in other studies; however, it is at the lower end of reported isotope effects. We speculate that differences in isotope effects reflect distinct nitrite oxidoreductase (NXR) enzyme orientations.

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Section: Nitrite Oxidation Kineticssupporting

confidence: 89%

Section: Nitrite Oxidation Kineticsmentioning

confidence: 60%

Section: Nitrite Oxidation Kineticsmentioning

confidence: 99%

See 1 more Smart Citation

Oxidation kinetics and inverse isotope effect of marine nitrite-oxidizing isolates

Jacob¹,

Nowka²,

Merten³

et al. 2017

Aquat. Microb. Ecol.

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“…The ammonia-oxidising population had a greater specific affinity for O 2 than the nitriteoxidising bacteria. Cessation of the oxygen supply resulted in an immediate accumulation of nitrite in the mixed cultures of Nitrosomonas europaea and Nitrobacter hamburgensis that had been growing at 80% O 2 saturation (Laanbroek et al 1994). Apparently the AOB win the competition for the limiting amounts of oxygen in the transient state between 80 and 0% O 2 when switched from oxic to anoxic.…”

Section: Dissolved Oxygen Concentration (Do)mentioning

confidence: 99%

“…Moreover, according to Hanaki et al (1990b) these low DO levels even result in higher (doubled) growth yields of AOB while the growth yield of NOB was unchanged, which can possibly give rise to increased nitrite concentrations. Experiments by Laanbroek et al (1994) resulted in yields of Nitrosomonas europaea at oxygen concentrations of 0 and 80% air saturation equal to 13.8 and 6.6 10 12 cells mol −1 NH + 4 respectively, which also implies more than a doubling of the growth yield for low DO levels. Ceçen and Gönenç (1995) found that the bulk oxygen to bulk ammonia ratio rather than the ammonia concentration itself is the most crucial parameter in the accumulation of nitrite.…”

Section: Dissolved Oxygen Concentration (Do)mentioning

confidence: 99%

Origin, causes and effects of increased nitrite concentrations in aquatic environments

Philips

Laanbroek

Verstraete

2002

Rev Environ Sci Biotechnol

Self Cite

312

198

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Literature frequently mentions increased nitrite concentrations along with its inhibitory effect towards bacteria and aquatic life. Nitrite accumulation has been studied for decades, and although numerous causal factors have already been commented on in literature, the mechanism of nitrite accumulation is not always clear. From the broad range of parameters and environmental factors reviewed in this paper, it is obvious that the causes and consequences of nitrite accumulation are not yet completely understood. Among others, pH, dissolved oxygen, volatile fatty acids, phosphate and reactor operation have been found to play a role in nitrite accumulation, which results from differential inhibition or disruption of the linkage of the different steps in both nitrification and denitrification. In the case of nitrification, this differential inhibition could lead to the displacement or unlinking of the ammonia oxidisers and nitrite oxidisers. In this paper, the idea is formulated that the nitrifier population forms a role model for the total microbial community. Increased nitrite concentrations would in this aspect not only signal a disruption of nitrifiers, but possibly also of the total configuration of the microbial community.Abbreviations: AMO -Ammonia mono-oxygenase; Anammox -Anaerobic ammonium oxidation; AOBAmmonia-oxidising bacteria; ATP -Adenosine triphosphate; COD -Chemical oxygen demand; DNRA -Dissimilatory nitrate reduction to ammonium; DO -Dissolved oxygen; EU -European union; FA -Free ammonia; F/M -Food to micro-organism ratio; FNA -Free nitrous acid; HAO -Hydroxylamine oxidoreductase; HRTHydraulic residence time; NDBEPR -Enhanced biological phosphate removal with nitrification and denitrification; NO -Nitric oxide; NOB -Nitrite-oxidising bacteria; NOR -Nitrite oxidoreductase; OLAND -Oxygen limited autotrophic nitrification denitrification; RBC -Rotating biological contactor; Sharon -Single reactor high activity ammonia removal over nitrite; SRT -Sludge residence time; TAN -Total ammoniacal nitrogen; TOC -Total organic carbon; VAS -Volatile attached solids

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Spatial and Temporal Dynamics of Dissolved Oxygen Concentrations and Bioactivity in the Hyporheic Zone

Reeder

Quick

Farrell

et al. 2018

Water Resources Research

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Dissolved oxygen (DO) concentrations and consumption rates are primary indicators of heterotrophic respiration and redox conditions in the hyporheic zone (HZ). Due to the complexity of hyporheic flow and interactions between hyporheic hydraulics and the biogeochemical processes, a detailed, mechanistic, and predictive understanding of the biogeochemical activity in the HZ has not yet been developed. Previous studies of microbial activity in the HZ have treated the metabolic DO consumption rate constant (KDO) as a temporally fixed and spatially homogeneous property that is determined primarily by the concentration of bioavailable carbon. These studies have generally treated bioactivity as temporally steady state, failing to capture the temporal dynamics of a changeable system. We demonstrate that hyporheic hydraulics controls rate constants in a hyporheic system that is relatively abundant in bioavailable carbon, such that KDO is a linear function of the local downwelling flux. We further demonstrate that, for triangular dunes, the downwelling velocities are lognormally distributed, as are the KDO values. By comparing measured and modeled DO profiles, we demonstrate that treating KDO as a function of the downwelling flux yields a significant improvement in the accuracy of predicted DO profiles. Additionally, our results demonstrate the temporal effect of carbon consumption on microbial respiration rates.

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Oxygen consumption kinetics of Nitrosomonas europaea and Nitrobacter hamburgensis grown in mixed continuous cultures at different oxygen concentrations

Cited by 36 publications

References 0 publications

Oxidation kinetics and inverse isotope effect of marine nitrite-oxidizing isolates

Oxidation kinetics and inverse isotope effect of marine nitrite-oxidizing isolates

Origin, causes and effects of increased nitrite concentrations in aquatic environments

Spatial and Temporal Dynamics of Dissolved Oxygen Concentrations and Bioactivity in the Hyporheic Zone

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