Oxygen consumption in relation to sorbitol utilization at the termination of diapause in eggs of the silkworm, Bombyx mori

Yaginuma, Toshinobu; Yamashita, Okitsugu

doi:10.1016/s0022-1910(98)00150-4

Cited by 37 publications

(17 citation statements)

References 30 publications

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“…It has been reported that oxygen consumption of silkworm diapause eggs abruptly decreases at 24 h post-oviposition at 251C (Yaginuma and Yamashita, 1999). Therefore, the peak of H 2 O 2 at 24 h may be due to the increase of oxygen consumption.…”

Section: Discussionmentioning

confidence: 93%

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Variations of hydrogen peroxide and catalase expression in Bombyx eggs during diapause initiation and termination

Sima

JinMei

Hou

et al. 2011

Arch Insect Biochem Physiol

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For diapause eggs of the silkworm, Bombyx mori, diapause initiation is prevented with hydrochloric acid (HCl) at around 20 h post-oviposition while diapause status is terminated with chilling around 5°C. To investigate whether hydrogen peroxide (H(2)O(2)) and catalase expression are involved in diapause initiation and termination, the concentration of H(2)O(2), relatively higher levels of catalase mRNA and activity of catalase were compared between (1) 20-h-old diapause eggs and the HCl-treated diapause eggs, and (2) 10-day-old diapause eggs and the 5°C-chilled diapause eggs. Compared to diapause eggs, the HCl-treated eggs had significantly higher H(2)O(2) concentrations (up from approximately 1-3 µmol/g fresh mass to 5-8 µmol/g fresh mass), higher relative level of catalase mRNA (up from 0 to 35.2%) and higher catalase activity (up from 2.51 units/mg protein to 4.97 units/mg protein) at 96 h post-treatment. On the other hand, the 5°C chilling resulted in significant increases of H(2)O(2) concentration (up from 0.79 µmol/g fresh mass to 5.57 µmol/g fresh mass), relative level of catalase mRNA (up from 0 to 71.4%) and catalase activity (up from 0.88 units/mg protein to 3.42 units/mg protein) within 120 days. The results obtained in this work suggest that variations of H(2)O(2) and catalase expression in Bombyx eggs are involved in diapause initiation and termination.

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Section: Discussionmentioning

confidence: 93%

“…It suggests that H 2 O 2 in diapause eggs may play an important role in diapause initiation. Oxygen consumption increases gradually five days after hydrochlorizing (Yaginuma and Yamashita, 1999). Therefore, enhancement of H 2 O 2 in the HCl-treated diapause eggs from 24 to 72 h may not be due to an increase in oxygen consumption.…”

Section: Discussionmentioning

confidence: 99%

Variations of hydrogen peroxide and catalase expression in Bombyx eggs during diapause initiation and termination

Sima

JinMei

Hou

et al. 2011

Arch Insect Biochem Physiol

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show abstract

“…The differentiation begins after oviposition and continues upto 4 days and organogenesis starts from the 4 th day and continues till hatching. These two stages show distinct variations in energy metabolism of diapause and non-diapause eggs (Yaginuma & Yamashita 1999). In the non-diapause eggs when incubated at 25…”

Section: Discussionmentioning

confidence: 99%

Expression of metabolic enzyme genes and heat-shock protein genes during embryonic development in diapause and non-diapause egg of multivoltine silkworm Bombyx mori

2008

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The expression of metabolic enzyme genes and heat-shock protein genes (Hsp) during early embryogenesis in diapause and non-diapause eggs of the silkworm Bombyx mori was quantified by semi-quantitative RT-PCR. The trehalase gene (Tre) was expressed in non-diapause eggs up-to nine days, while in diapause eggs was not up regulated. The glycogen phosphorylase gene (GPase) was expressed in non-diapause eggs, whereas in diapause eggs a high level was observed in early stage, but down regulated in later stage. The phosphofructokinase gene (PFK) and sorbitol dehyrogenase-2 gene (SDH-2) expression was fluctuated in non-diapause eggs, whereas in diapause eggs these were expressed only at early stage and not observed in later stage. The glucose-6-phosphate dehydrogenase gene (G6P-DH) in non-diapause eggs was highly expressed during the differentiation phase and decreased in the organogenesis phase. In contrast to this, expression in diapause eggs was of low level during differentiation phase and of high level observed in the organogenesis phase. In the tissues, PFK and SDH-2 were selectively expressed in cuticle and midgut, whereas Tre expression was high in midgut and ovary of larvae incubated at 15 (20.4, 20.8, 40, 70, and 90) were expressed in both diapause and non-diapause eggs. Their expression was, however, selective in tissues with Hsp20.4 in midgut and ovary, Hsp40 in head, Hsp70 in cuticle and Hsp90 in ovary and head in high amounts at 15• C. These results suggest that the metabolic enzyme genes studied except Hsp play a major role during embryogenesis of diapause and non-diapause silkworm.

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“…Diapause most likely underlies the variation among queens that may lead to differences in the ability to produce offspring (Beekmand and Vanstratum, 2000). Reduced oxygen consumption is generally regarded as a characteristic phenomenon of insect diapause (Yaginuma and Yamashita, 1999). Studies on oxygen consumption during insect diapause have been performed on eggs of the domestic silkworm (Chino, 1958), Cecropia silkworm (Schneiderman and Williams, 1953), Colorado potato beetles , and other diapause insects (Waku, 1965;Ellingsen, 1978;Crozier, 1979).…”

Section: Indoor Rearingmentioning

confidence: 99%

Optimal Cold Temperature for the Artificial Hibernation of Bombus ignitus Queen Bumblebees

Yoon¹,

Lee²,

Kim³

et al. 2013

International Journal of Industrial Entomology

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Bumblebees are widely used to pollinate various greenhouse crops. Among the different bumblebee species, Bombus ignitus is indigenous to Korea, China, Japan and Russia. B. ignitus undergoes one generation per year, and artificial hibernation is essential for year-round rearing of the bumblebee. Keeping the queens under low-temperature conditions for several months is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how cold temperature affects the artificial hibernation of B. ignitus queens. Under chilling temperatures of -2.5°C, 0°C, 2.5°C and 5°C with constant humidity >80%, the queens stored at 2.5°C exhibited the highest survival rates, which were 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months and 24.0% at 6 months. Lower survival rates were observed at 0°C, 5°C, 7.5°C and 12.5°C. At 2.5°C the colony developmental characteristics after diapause were 1.2-to 1.5-fold greater than those when queens were stored at 5°C. Thus, 2.5°C and 70% R.H.were the most favorable chilling temperature and humidity conditions for terminating the diapause of B. ignitus queens. Introduction AbstractInt.

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Oxygen consumption in relation to sorbitol utilization at the termination of diapause in eggs of the silkworm, Bombyx mori

Cited by 37 publications

References 30 publications

Variations of hydrogen peroxide and catalase expression in Bombyx eggs during diapause initiation and termination

Variations of hydrogen peroxide and catalase expression in Bombyx eggs during diapause initiation and termination

Expression of metabolic enzyme genes and heat-shock protein genes during embryonic development in diapause and non-diapause egg of multivoltine silkworm Bombyx mori

Optimal Cold Temperature for the Artificial Hibernation of Bombus ignitus Queen Bumblebees

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