Oxygen consumption by the isolated smooth muscle of guinea‐pig taenia coli

Abstract

SUMMARY1. An apparatus is described for simultaneous measurement of oxygen consumption and electrical and mechanical activity of isolated smooth muscle preparations.2. The mean oxygen uptake by the isolated taenia coli of the guineapig was 10-20 L./g/min.3. In spontaneously active preparations, adrenaline (10-8-10-7 g/ml.) caused, with the inhibition of electrical and mechanical activity, a reduction in oxygen uptake.4. After prolonged exposure to substrate free solution spontaneous activity ceased periodically. Adrenaline, when applied during a silent period, had no detectable effect on resting oxygen consumption, while readmission of substrate, either glucose or /5-hydroxybutyrate, increased oxygen uptake.5. Adrenaline did not modify the increased oxygen uptake during the initial recovery period when it was given simultaneously with the substrate. However, adrenaline shortened the time interval which elapsed from the addition of substrate until spontaneous activity was resumed, indicating an acceleration of the recovery process.

Effects of Calcium Depletion on Oxygen Consumption in Guinea Pig Taenia Coli

“…In smooth muscle of guinea pig taenia coli, showing spontaneous activity, the curves of rhythmic changes in muscle tension appears parallel to the curves of changes in Pot level in the medium (1)(2)(3).…”

Oxygen Consumption During Potassium Induced Contracture in Guinea Pig Taenia Coli

“…Notwithstanding similarities our apparatus was devised quite independent of their work (4), and some of the data obtained using our apparatus has already been published (5).…”

The effects of isoprenaline and phenylephrine on oxygen consumption in isolated smooth muscle

“…However, isoprenaline and phenylephrine (this study) and adrenaline (Bulbring & Golenhofen, 1967) failed to produce changes in oxygen consumption when administered during periods of mechanical quiescence in guinea-pig taenia coli. These observations further suggest that, in mechanically active preparations, the metabolic changes were the result of altered mechanical activity and did not involve a direct metabolic action of the drugs.…”

“…An increase in oxygen consumption accompanied the increase in tension produced spontaneously (Billbring, 1953;Bulbring & Golenhofen, 1967; this study), by acetylcholine (Builbring, 1953; this study), by histamine (Biilbring, 1953), by potassium ions (Saito, Sakai, Ikeda & Urakawa, 1968), and by activation of a-adrenoceptors (this study) and /8-adrenoceptors (Williamson, 1966). A decrease in tension produced spontaneously (Bulbring & Golenhofen, 1967;this study) or by activation of a-or /3-adrenoceptors was accompanied by a reduction in oxygen consumption.…”