Oxygen consumption and metabolic responses of freshwater crab Sinopotamon henanense to acute and sub-chronic cadmium exposure

Xuan, Rongrong; Wu, Hao; Lin, Changdong; Ma, Dandan; Li, Yingjun; Xu, Tao; Wang, Lan

doi:10.1016/j.ecoenv.2012.10.022

Cited by 21 publications

(21 citation statements)

References 31 publications

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“…Crustaceans are sensitive to heavy metal pollution, and crabs have been considered as suitable bioindicators 5 6 9 10 16 17 18 19 20 21 . Among them, freshwater crab, Sinopotamon henanense , is an important representative species of decapod crustaceans.…”

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confidence: 99%

“…Among them, freshwater crab, Sinopotamon henanense , is an important representative species of decapod crustaceans. Because it lives in the sediments of aquatic environments and is directly exposed to heavy metals, the crab has been confirmed to accumulate Cd in its main organs such as hepatopancreas, gill, gonad, and hemocytes 11 12 17 18 19 20 21 . In crustaceans, hepatopancreas is generally thought to be a key target organ for heavy metal toxicity and other environmental stresses 17 18 19 20 22 23 24 25 .…”

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confidence: 99%

“…Because it lives in the sediments of aquatic environments and is directly exposed to heavy metals, the crab has been confirmed to accumulate Cd in its main organs such as hepatopancreas, gill, gonad, and hemocytes 11 12 17 18 19 20 21 . In crustaceans, hepatopancreas is generally thought to be a key target organ for heavy metal toxicity and other environmental stresses 17 18 19 20 22 23 24 25 . Besides its functions in digestion and metabolism 19 20 26 27 28 , hepatopancreas also plays an important role in response to environmental stresses 17 18 19 20 24 25 29 .…”

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confidence: 99%

“…In crustaceans, hepatopancreas is generally thought to be a key target organ for heavy metal toxicity and other environmental stresses 17 18 19 20 22 23 24 25 . Besides its functions in digestion and metabolism 19 20 26 27 28 , hepatopancreas also plays an important role in response to environmental stresses 17 18 19 20 24 25 29 . Previous studies have demonstrated that crabs have a compensatory metabolism with an increase of protease and aminotransferase activities of hepatopancreas and muscle in response to energy stress by acute Cd exposure 19 .…”

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Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense

Sun

Liu

et al. 2016

Sci Rep

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Cadmium (Cd) pollution is a serious global problem, which causes irreversible toxic effects on animals. Freshwater crab, Sinopotamon henanense, is a useful environmental indicator since it is widely distributed in benthic habitats whereby it tends to accumulate Cd and other toxicants. However, its molecular responses to Cd toxicity remain unclear. In this study, we performed transcriptome sequencing and gene expression analyses of its hepatopancreas with and without Cd treatments. A total of 7.78 G clean reads were obtained from the pooled samples, and 68,648 unigenes with an average size of 622 bp were assembled, in which 5,436 were metabolism-associated and 2,728 were stimulus response-associated that include 380 immunity-related unigenes. Expression profile analysis demonstrated that most genes involved in macromolecular metabolism, oxidative phosphorylation, detoxification and anti-oxidant defense were up-regulated by Cd exposure, whereas immunity-related genes were down-regulated, except the genes involved in phagocytosis were up-regulated. The current data indicate that Cd exposure alters gene expressions in a concentration-dependent manner. Therefore, our results provide the first comprehensive S.henanense transcriptome dataset, which is useful for biological and ecotoxicological studies on this crab and its related species at molecular level, and some key Cd-responsive genes may provide candidate biomarkers for monitoring aquatic pollution by heavy metals.

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Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense

Sun

Liu

et al. 2016

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“…S. paramamosain living in water is easily impacted by light intensity and the impacts of different light intensities on aquatic crustaceans have received some attention in recent years. One of the main functions of the hepatopancreas is metabolism [28,29]. This study focuses on using transcriptome technology to investigate metabolism gene expression changes in the hepatopancreas of S. paramamosain in different light intensities.…”

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Transcriptome Analysis of Genes and Pathways Associated With Metabolism in Scylla Paramamosain Under Different Light Intensities During Indoor Overwintering

Zhou

Wang

et al. 2020

Preprint

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Background: Scylla paramamosain is one of the commercially crucial marine crustaceans belonging to the genus Scylla, which is commonly distributed along the coasts of China, Vietnam, and Japan. Genomic and transcriptomic data are scarce for the mud crab. Light intensity is one of the ecological factors that affect S. paramamosain during indoor overwintering. Methods: To understand the energy metabolism mechanism adapted to light intensity, we analyzed the transcriptome of S. paramamosain hepatopancreas in response to different light intensities (0, 1.43, 40.31 μmol·m-2·s-1). Results: A total of 5052 differentially expressed genes were identified in the LL group (3104 genes were up-regulated and 1948 genes were down-regulated). A total of 7403 differentially expressed genes were identified in the HL group (5262 genes were up-regulated and 2141 genes were down-regulated). Conclusion: The results showed that S. paramamosain adapts to different light intensity environments through the regulation of amino acids, fatty acids, carbon and energy metabolism. Different light intensities had a strong impact on the energy generation of S. paramamosain by influencing oxygen consumption rate, aerobic respiration, glycolysis/gluconeogenesis pathway, the citrate cycle (TCA cycle) and fatty acid degradation. The results of the current study showed that S. paramamosain produced more energy under high light intensities. The findings of the study add to the knowledge of regulatory mechanisms related to S. paramamosain metabolism under different light intensities.

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Cadmium inhibits the vitellogenesis of freshwater crab Sinopotamon henanense

Yang

Dahms‬

et al. 2015

Enviro Toxic and Chemistry

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Cadmium (Cd) may pose risks to freshwater organisms, including crabs that live at the interface of sediments and water column all year round. One of the major changes that occur during oocyte maturation of crabs is the production of vitellin. In the present study, the authors investigated the effects of Cd on oocyte size, vitellin level, and vitellogenin (Vtg) messenger RNA (mRNA) expression in the ovary of the freshwater crab Sinopotamon henanense. The authors studied the impacts of Cd on carbohydrate as well as on the protein metabolism, metallothionein, glutathione (GSH) synthesis, energy-related parameters, and mRNA expression of genes involved in energy metabolism. After Cd treatment, vitellin concentration, Vtg mRNA expression, and oocyte diameter decreased. Less carbohydrate and enhanced protein catabolism were found in the ovary. Adenosine triphosphate to adenosine diphosphate (ATP:ADP) ratios, reduced nicotinamide adenine dinucleotide (NADH) to oxidized nicotinamide adenine dinucleotide (NAD(+) ) ratios, mitochondrial membrane potential, and energy production-related mRNA expressions increased in the ovary after 10 d Cd treatment and decreased after 20 d. Metallothionein and GSH synthesis were up-regulated after 10 d on Cd exposure. Moreover, Cd caused a time-dependent up-regulation of malondialdehyde. The authors' findings show significant effects of Cd on vitellogenesis, which suggests that Cd slows down vitellogenesis in S. henanense because of excessive energy consumption and an activated defense system.

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Oxygen consumption and metabolic responses of freshwater crab Sinopotamon henanense to acute and sub-chronic cadmium exposure

Cited by 21 publications

References 31 publications

Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense

Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense

Transcriptome Analysis of Genes and Pathways Associated With Metabolism in Scylla Paramamosain Under Different Light Intensities During Indoor Overwintering

Cadmium inhibits the vitellogenesis of freshwater crab Sinopotamon henanense

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