Schwertmannite (Sch) is an efficient adsorbent for arsenic(III) removal from arsenic(III)-contaminated groundwater. In this study, bio-schertmannite was synthesized in the presence of dissolved ferrous ions and Acidithiobacillus ferrooxidans LX5 in a culture media. Bio-synthesized Sch characteristics, such as total organic carbon (TOC), morphology, chemical functional groups, mineral phase, specific surface area, and pore volume were systematically studied after it was dried at 105 • C and then heated at 250-550 • C. Differences in arsenic(III) removal efficiency between 105 • C dried-sch and 250-550 • C heated-sch also were investigated. The results showed that total organic carbon content in Sch and Sch weight gradually decreased when temperature increased from 105 • C to 350 • C. Sch partly transformed to another nanocrystalline or amorphous phase above 350 • C. The specific surface area of 250 • C heated-sch was 110.06 m 2 /g compared to 5.14 m 2 /g for the 105 • C dried-sch. Total pore volume of 105 • C dried-sch was 0.025 cm 3 /g with 32.0% mesopore and 68.0% macropore. However, total pore volume of 250 • C heated-mineral was 0.106 cm 3 /g with 23.6% micropore, 33.0% mesopore, and 43.4% macropore. The arsenic(III) removal efficiency from an initial 1 mg/L arsenic(III) solution (pH 7.5) was 25.1% when 0.25 g/L of 105 • C dried-sch was used as adsorbent. However, this efficiency increased to 93.0% when using 250 • C heated-sch as adsorbent. Finally, the highest efficiency for arsenic(III) removal was obtained with sch-250 • C due to high amounts of sorption sites in agreement with the high specific surface area (SSA) obtained for this sample.