Oxime as a general photocage for the design of visible light photo-activatable fluorophores

Abstract

Photoactivatable fluorophores have been widely used for tracking molecular and cellular dynamics with subdiffraction resolution.

Xanthone-based solvatochromic fluorophores for quantifying micropolarity of protein aggregates

“…40 nm), resulting in poor sensitivity to changes in polarity. In recent years, elegant strategies based on single-atom replacement have been applied to different fluorophore scaffolds to obtain fluorescent dyes with larger emission shifts [47][48][49][50] . Learning from these studies, our rationale has been to develop a single-atom replacement strategy for designing minimally-modified solvatochromic probes capable of detecting changes in micro-polarity that occur during protein aggregation in live cells.…”

A Universal Photoactivatable Tag Attached to Fluorophores Enables Their Use for Single‐Molecule Imaging

“…According to the fluorescence quantum yield results, Coumarin‐OP, BODIPY‐OP, Rhodamine‐OP and Cy5‐OP showed a 108‐, 37‐, 109‐, and 105‐fold enhancement compared to their corresponding SO conjugated dyes, respectively (Table 1). We also measured the photoconversion quantum yields by a previously reported method, [5] the details were shown in Supporting Information. The relatively low photoconversion quantum yields (Table 1) indicated a slow turn‐on process, suggesting their potential for localization‐based super‐resolution imaging where sparsity of fluorescence is required and high‐power density (≈kW cm −2 ) is used.…”

“…[3,4] Moreover, by controlling the onset and the site of photoactivation, the target of interest can be probed and monitored in real-time to reveal molecular or cellular dynamics. [5][6][7] As a result, designing and constructing novel photoactivatable dyes will continuously bring new opportunities in the structural and functional study of biomolecular systems.…”

“…Images with sub‐diffraction resolution can be achieved by repetitive photoactivation of a sparse subset of labeled fluorophores and reconstructing the positions of single fluorophores [3, 4] . Moreover, by controlling the onset and the site of photoactivation, the target of interest can be probed and monitored in real‐time to reveal molecular or cellular dynamics [5–7] . As a result, designing and constructing novel photoactivatable dyes will continuously bring new opportunities in the structural and functional study of biomolecular systems.…”

A tetrazole-ene photoactivatable fluorophore with improved brightness and stability in protic solution

“…Photoactivatable fluorophores, as turn-on fluorophores activated by light, are valuable tools for tracking the dynamics of biological processes and localization fluorescence microscopy. [1][2][3][4][5] One general strategy for designing photoactivatable fluorophores is photo-uncaging of the ''caged'' fluorophores such as fluorescein, coumarin, and rhodamine to regenerate the fluorophores [5][6][7][8][9][10] in their active form. Another group of photoactivatable fluorophores were designed based on light-induced bond-breaking and thermal bond-reforming.…”