Oxidoreductase activity of sorghum root exudates in a phenanthrene-contaminated environment

Muratova, A. Yu.; Pozdnyakova, N. N.; Голубев, С. А.; Wittenmayer, Lutz; Makarov, O. E.; Merbach, W.; Turkovskaya, O. V.

doi:10.1016/j.chemosphere.2008.11.011

Cited by 45 publications

(24 citation statements)

References 34 publications

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“…They found phenanthrene more phytotoxic than pyrene and wheat the most sensitive species. Phytotoxicity is in fact due to an excessive accumulation of phenanthrene in plant tissues and plants respond to pollutants only after being exposed to the "threshold concentration" that varies with both pollutant and species (Muratova et al, 2009). In this context, it was established that a fast accumulation phase of phenanthrene occurs during the first hours (the first two hours in the case of wheat) of treatment, followed by a slower one.…”

Section: Discussionmentioning

confidence: 99%

“…This suggests that Thellungiella salsuginea and Arabidopsis thaliana accumulated high quantities of the pollutant during the first hours of treatment, which can explain the noticeable reduction in their fresh weights. But, the differences in their responses to the stress in terms of biomass production can be attributed to a probable lower accumulation and/or a better management of the accumulated quantity of phenanthrene by the halophyte in comparison with the glycophyte, including metabolization by cell-enzymes, vacuole-sequestration, and volatilization by stomata (Muratova et al, 2009). The lipophilic PAH nature can indirectly disturb biomembrane structure as a consequence of the formation of free radicals induced by these molecules and their derivatives (Tukaj and Aksmann, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Responses of glycophytes to PAHs were well studied and most of them showed significant sensitivity to these constraints (Liu et al, 2009;Muratova et al, 2009;Song et al, 2005;Weisman et al, 2010). In this context, several investigations were performed on the model plant Arabidopsis thaliana for a better understanding of the mechanisms involved in PAH tolerance with the aim to identify selection criteria or useful tools in phytoremediation and/or amelioration programs thanks to its high intraspecific diversity and to the abundance of its developed molecular tools.…”

Section: Introductionmentioning

confidence: 99%

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The halophytic model plant Thellungiella salsuginea exhibited increased tolerance to phenanthrene-induced stress in comparison with the glycophitic one Arabidopsis thaliana: Application for phytoremediation

Shiri

Rabhi

Abdelly

et al. 2015

Ecological Engineering

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Polycyclic aromatic hydrocarbons constitute a large family of organic environmental pollutants. Hence, a particular attention has been attributed to all approaches involved in the reduction of their contamination in water and natural ecosystems. Plant tolerance, absorption, accumulation, and likely biodegradation of these pollutants, known as phytoremediation, have emerged as an efficient technique to remediate environments contaminated with polycyclic aromatic hydrocarbons. The present work was aimed to compare the tolerance to phenanthrene (a polycyclic aromatic hydrocarbon) in the two model plants: Arabidopsis thaliana (glycophyte) and Thellungiella salsuginea (halophyte). Our study showed that the development of these two species was reduced under phenanthrene stress, the effect being more pronounced in Arabidopsis thaliana than in Thellungiella salsuginea. In parallel, results from the intrinsic quantum yield of photosystem II and chlorophyll concentrations were concomitant with those of growth and phenotypic changes, and confirmed the higher tolerance of Thellungiella salsuginea compared to that of Arabidopsis thaliana. The intrinsic quantum yield of photosystem II was drastically decreased in the glycophyte, which indicates a marked disturbance in photosystem II performance. This induced a severe oxidative stress as shown by the utilization of specific reactive oxygen species probes. In parallel, the activities of glutathione reductase, gaiacol peroxidase, and superoxide dismutase were increased by 95, 73, and 36%, respectively, which indicates a marked phenanthrene-induced oxidative stress. In Thellungiella salsuginea, photosystem II performance was not significantly affected. This species showed less accumulated reactive oxygen species than Arabidopsis thaliana. Its enzymatic antioxidant system showed few changes as superoxide dismutase was the only enzyme whose activity was enhanced (+34%). A 3 much higher capacity of recovery was also noticed in this halophyte as compared to the glycophyte. Indeed, it seems that Thellungiella salsuginea accumulated phenanthrene in stomata, which suggests its possible volatilization. All these data, taken together, add new insight to the mechanisms involved in halophytic plant tolerance to abiotic stresses and their potential use in phytoremediation. Graphical abstract Phenanthrene Glycophytic

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The halophytic model plant Thellungiella salsuginea exhibited increased tolerance to phenanthrene-induced stress in comparison with the glycophitic one Arabidopsis thaliana: Application for phytoremediation

Shiri

Rabhi

Abdelly

et al. 2015

Ecological Engineering

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“…Using lyophilized samples study, it was concluded that as a result of the enzymatic activity of the root exudates, some of the PAHs and products of PAH degradation were oxidized in the reaction mixture supplemented with the mediating agents, but that no oxidation was observed in the reaction mixtures without the mediators. The revealed enzymatic activity of the sorghum root exudates may indicate the involvement of the root -released oxidoreductases in rhizospheric degradation of PAHs and/or their derivatives (Muratovaa et al, 2009).…”

Section: Approaches Of Remediationmentioning

confidence: 99%

Nature and role of root exudates: Efficacy in bioremediation

Shukla¹,

Sharma²,

Singh³

et al. 2013

Afr. J. Biotechnol.

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“…In plant roots, the bulk of the PO is localized in the primary cell wall but also in membranes, organelles, vacuoles, and the cytoplasm [33]. Class (iii) peroxidases could thus be leached from roots of cotton, wheat, cress, tomato, water hyacinth, French bean, rice [33,35] and other plants [15,30,36]. Taken with the chromogen guaiacol at pH 7.0, alfalfa plants but not oilseed rape surpassed the root exuded PO activities of white mustard 167-182 times both in axenic and natural soil cultures [15,37].…”

Section: Introductionmentioning

confidence: 99%

Formation of the Azodication (ABTS2+) from ABTS [2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulphonate)] in Sterile Plant Cultures: Root–Exuded Oxidoreductases Contribute to Rhizosphere Priming

Gramss

2018

Soil Systems

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Abstract:Rhizosphere priming by terrestrial plants comprises increased or repressed efflux of CO 2 and N from soil organic matter (SOM), decaying under the impact of temperature, moisture, and the composition of rhizodeposits. Contemporarily, increases in water solubility vs. losses in molecular size, aromaticity, and the content in phenolic OH groups denote the degradation of SOM in planted soil. Root peroxidases (POs) and 'polyphenoloxidases' are surmised to contribute to these effects, however, final evidence for this is lacking. Therefore, seedlings of white mustard, alfalfa, and oilseed rape with wide spans in PO release were grown in hydroponic cultures at variable levels of Cu/Fe/Mn as Fenton metals, but also under P and Fe starvation to stimulate the release of carboxylic acids that form catalytic Mn 3+ chelants from Mn 2+ and MnO 2 . The shortage in active oxygen as a cosubstrate of POs delayed the immediate oxidation of 2,2 -azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS) supplements to the green ABTS •+ by PO/H 2 O 2 , the possible formation of Mn 3+ via PO catalyzed aryloxy radicals from root-released phenolics, and of HO • by metal cations in H 2 O 2 dependent Fenton-like reactions. Enhanced by exuded and external malate, O 2 independent MnO 2 supplements in some treatments formed ABTS •+ spontaneously. The culture fluids then turned red in all treatments within 24-60 h by the formation of azodication (ABTS 2+ ) derivatives in a second plant initiated oxidation step that is known to be catalyzed by substrate radicals. It is concluded that plants initiate oxidative activities that contribute to rhizosphere priming in an environment of oxidoreductase and carboxylate exudates, the indicated presence of mediating substrate radicals, and the cations and (hydr)oxides of transition metals. Pathways of H 2 O 2 production upon the degradation of carboxylates and by the POs themselves are indicated.

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Oxidoreductase activity of sorghum root exudates in a phenanthrene-contaminated environment

Cited by 45 publications

References 34 publications

The halophytic model plant Thellungiella salsuginea exhibited increased tolerance to phenanthrene-induced stress in comparison with the glycophitic one Arabidopsis thaliana: Application for phytoremediation

The halophytic model plant Thellungiella salsuginea exhibited increased tolerance to phenanthrene-induced stress in comparison with the glycophitic one Arabidopsis thaliana: Application for phytoremediation

Nature and role of root exudates: Efficacy in bioremediation

Formation of the Azodication (ABTS2+) from ABTS [2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulphonate)] in Sterile Plant Cultures: Root–Exuded Oxidoreductases Contribute to Rhizosphere Priming

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