Oxidized low density lipoprotein stimulates collagen production in cultured arterial smooth muscle cells

Jimi, Shiro; Saku, Keijiro; Uesugi, Noriko; Sakata, Noriyuki; Takebayashi, Shigeo

doi:10.1016/0021-9150(95)05515-x

Cited by 55 publications

(40 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…OxLDL has been shown to induce tissue factor gene expression mediated by both early growth response protein 1 (Egr-1) and specificity protein 1 (Sp1) in SMCs (77). Collagen production by SMCs is stimulated by oxLDL but not native LDL, suggesting that elevations in oxLDL could lead to collagenosis in atherosclerosis (159). OxLDL may also promote osteogenic differentiation of VSMCs and thus vascular calcification (35).…”

Section: B Oxldl Is One Of the Most Important Risk Factors For Athermentioning

confidence: 99%

The Human Paraoxonase Gene Cluster As a Target in the Treatment of Atherosclerosis

She

Chen

Yan

et al. 2012

Antioxidants & Redox Signaling

View full text Add to dashboard Cite

The paraoxonase (PON) gene cluster contains three adjacent gene members, PON1, PON2, and PON3. Originating from the same fungus lactonase precursor, all of the three PON genes share high sequence identity and a similar b propeller protein structure. PON1 and PON3 are primarily expressed in the liver and secreted into the serum upon expression, whereas PON2 is ubiquitously expressed and remains inside the cell. Each PON member has high catalytic activity toward corresponding artificial organophosphate, and all exhibit activities to lactones. Therefore, all three members of the family are regarded as lactonases. Under physiological conditions, they act to degrade metabolites of polyunsaturated fatty acids and homocysteine (Hcy) thiolactone, among other compounds. By detoxifying both oxidized low-density lipoprotein and Hcy thiolactone, PONs protect against atherosclerosis and coronary artery diseases, as has been illustrated by many types of in vitro and in vivo experimental evidence. Clinical observations focusing on gene polymorphisms also indicate that PON1, PON2, and PON3 are protective against coronary artery disease. Many other conditions, such as diabetes, metabolic syndrome, and aging, have been shown to relate to PONs. The abundance and/or activity of PONs can be regulated by lipoproteins and their metabolites, biological macromolecules, pharmacological treatments, dietary factors, and lifestyle. In conclusion, both previous results and ongoing studies provide evidence, making the PON cluster a prospective target for the treatment of atherosclerosis. Antioxid. Redox Signal. 16, 597-632.

show abstract

Section: B Oxldl Is One Of the Most Important Risk Factors For Athermentioning

confidence: 99%

The Human Paraoxonase Gene Cluster As a Target in the Treatment of Atherosclerosis

She

Chen

Yan

et al. 2012

Antioxidants & Redox Signaling

View full text Add to dashboard Cite

show abstract

“…8,9,53 Moreover, ascorbic acid increases LDL degradation in cultured vascular smooth muscle cells 54 and alters their production of extracellular matrix basally 55 and after treatment with oxLDL. 56 These actions may explain the antiatherogenic potential of ascorbic acid in reducing intimal thickening and LDL levels in the subendothelial space.…”

mentioning

confidence: 99%

Vitamin C Protects Human Arterial Smooth Muscle Cells Against Atherogenic Lipoproteins

Siow

Sato²,

Leake³

et al. 1998

ATVB

View full text Add to dashboard Cite

Abstract-Glutathione (GSH) plays a key role in cellular antioxidant defenses by scavenging reactive oxygen species and reducing lipid peroxides. Intracellular GSH levels are regulated by transport of its precursor L-cystine via system x c Ϫ , which can be induced by oxidant stress. As oxidatively modified low density lipoproteins (LDLs) contribute to impaired vascular reactivity and the formation of atherosclerotic lesions, we have examined the effects of oxidized LDL and the antioxidant vitamins C and E on the L-cystine-GSH pathway in human umbilical artery smooth muscle cells (HUASMCs). Oxidized LDL, but not native LDL, elevated intracellular GSH levels and L-cystine transport via system x c Ϫ in a time-dependent (up to 24 hours) and dose-dependent (10 to 100 g ⅐ mL Ϫ1 ) manner. These increases were dependent on protein synthesis and the extent of LDL oxidation, but the induction of L-cystine transport activity was independent of GSH synthesis. Pretreatment of HUASMCs for 24 hours with vitamin E (100 mol/L) attenuated oxidized LDL-mediated increases in GSH, whereas pretreatment with vitamin C depressed basal levels and abolished oxidized LDL-induced increases in GSH and L-cystine transport in a time-dependent (3 to 24 hours) and dose-dependent (10 to 100 mol/L) manner. Pretreatment of cells with dehydroascorbate had no effect on oxidized LDL-mediated increases in L-cystine transport and only marginally attenuated increases in GSH. Our findings provide the first evidence that vitamin C spares endogenous adaptive antioxidant responses in human vascular smooth muscle cells exposed to atherogenic oxidized LDL.

show abstract

“…The balance between oxidants and anti-oxidants is important in the pathophysiology of AS as well as several other conditions 19) . For example, previous studies have shown that oxidized-LDL stimulates collagen synthesis in arterial smooth muscle cells and causes intimal thickening, which may contribute to AS 20) . PON1 is an enzyme synthesized in the liver and secreted into the blood, where it mainly binds to high density lipoproteins 21) .…”

Section: Discussionmentioning

confidence: 99%

Serum Paraoxonase 1 Activity Predicts Arterial Stiffness in Renal Transplant Recipients

Güngör

Kırçelli

Demirci

et al. 2011

JAT

View full text Add to dashboard Cite

Aim: Paraoxanase 1 (PON 1) has been shown to protect against atherosclerosis by modifying lipoproteins. Its activity decreases in dialysis patients but is restored after transplantation. Whether it affects arterial stiffness is unclear. In this study we aimed to investigate the effects of PON 1 on arterial stiffness in renal transplant patients. Methods: Seventy renal transplant recipients were enrolled. Arterial stiffness was measured using a Syphmocor device. PON-1 activity was assessed from the rate of enzymatic hydrolysis of paraoxon to p-nitrophenol.

show abstract

Oxidized low density lipoprotein stimulates collagen production in cultured arterial smooth muscle cells

Cited by 55 publications

References 31 publications

The Human Paraoxonase Gene Cluster As a Target in the Treatment of Atherosclerosis

The Human Paraoxonase Gene Cluster As a Target in the Treatment of Atherosclerosis

Vitamin C Protects Human Arterial Smooth Muscle Cells Against Atherogenic Lipoproteins

Serum Paraoxonase 1 Activity Predicts Arterial Stiffness in Renal Transplant Recipients

Contact Info

Product

Resources

About