Oxidized LDL and its correlation with lipid profile and oxidative stress biomarkers in young healthy Spanish subjects

Abstract

The biological effects of oxidized LDL (oxLDL) may contribute to initiation and progression of the atherosclerotic process, and the association between cardiovascular disease and oxidation of LDL has been largely demonstrated. The objectives of this study were to establish the reference values of oxidative stress biomarkers in a young healthy Spanish population to determine the concentration of oxLDL and its relationship with lipid profile and with these biomarkers. oxLDL, F(2)-isoprostanes, protein carbonyls (PC), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were determinate by ELISA in 72 healthy subjects. Antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) were carried out on a Hitachi 912 analyzer; lipid profile were assayed using automated systems (Cobas 711, Roche Diagnostics). All statistics were analyzed by using SPSS for Windows 15.0. SPSS Inc, Chicago, IL, USA. (Normal mean reference values): oxLDL (63.23 +/- 16.23 U/L), (Male/Female 68.06 +/- 17.69/58.39 +/- 13.6 U/L), F(2)-isoprostanes (2.26 +/- 0.9 microg/g creatinine), PC (0.34 +/- 0.15 nmol/mg), 8-OHdG (23.27 +/- 10.58 ng/ml), SOD (931.97 +/- 271.09 U/g Hb), GR (46.56 +/- 11.68 U/L), GPx (27.58 +/- 6.89 U/gHb (Male/Female 25.91 +/- 5.03/29.2 +/- 8.07 U/L)). OxLDL (63.23 U/L) was significantly (p < 0.05) positively correlated with BMI (22.53 Kg/m(2)), total cholesterol (175.79 mg/dl), triglycerides (87.58 mg/dl), LDL cholesterol (96.25 mg/dl), and uric acid (4.78 mg/dl), while negatively correlated with HDL-cholesterol (62.25 mg/dl). We have found different correlation between oxLDL and isoprostanes by gender with the rest of parameters. Normal reference values have been found significantly different for oxLDL and GPx by gender. Oxidized LDL is correlated with lipid profile but not with the oxidative stress biomarkers. Urinary isoprostanes are positively correlated with triglycerides and negatively with GR and GPx.

The <b><i>MTHFR</i></b> C677T Polymorphism Is Related to Plasma Concentration of Oxidized Low-Density Lipoprotein in Adolescents with Cardiovascular Risk Factors

“…On the other hand, Koubaa et al [39] did not find any difference in ox-LDL levels among Tunisian carriers of the C677T variant allele. The mean ox-LDL concentrations verified in our study were lower than those observed in adolescents and young adults with severe obesity and greater oxidative stress degree [40,41]. …”

Impact of obesity and Ala16Val MnSOD polymorphism interaction on lipid, inflammatory and oxidative blood biomarkers

“…A positive correlation of OxLDL concentration with cholesterol levels and body mass index, has been reported in previous studies [30,33,37] indicating that metabolic syndrome and their components as obesity exacerbates oxidized LDL levels in a feedback loop [39]. The results found in the corroborates the relevance of oxidative stress status on OxLDL levels.…”

“…These results are not new since a large body of previous evidences has described the influence of obesity on lipid, oxidative and inflammatory biomarkers [30,36,37]. However, in our study we found that the alterations in these biomarkers can be influenced by the genetic status of volunteers considering the Ala16Val MnSOD polymorphism.…”

Intake of Red Wine in Different Meals Modulates Oxidized LDL Level, Oxidative and Inflammatory Gene Expression in Healthy People: A Randomized Crossover Trial

“…Indeed small LDL particles themselves are easily oxidized to yield atherogenic oxidized LDL (ox-LDL) particles, also detectable in healthy subjects [5, 6]. Elevated levels of ox-LDL particles in blood stream have been reported to be associated with increased cardiovascular disease risk [6, 7]. …”