Oxidisability of low density lipoproteins in patients with carotid or femoral artery atherosclerosis

Andrews, Brian T.; Burnand, K. G.; Paganga, George; Browse, N L; Rice‐Evans, Catherine; Sommerville, K.; Leake, David S.; Taub, Nicholas

doi:10.1016/0021-9150(94)05401-4

Cited by 49 publications

(23 citation statements)

References 10 publications

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“…Multiple linear regression analysis of changes in platelet lipids as dependent variables with changes in plasma phospholipid eicosapentaenoic and docosahexaenoic acid as independent variables* † which is putatively more susceptible to oxidation (DeGraaf et al 1991), is a defining feature. Although it remains controversial whether or not LDL oxidizability is a significant determinant of CHD risk (Regnstrom et al 1992;Andrews et al 1995;Toshima et al 2000), many consider oxidized LDL to be important in the initiation and progression of atherogenesis (Berliner et al 1990;Weis et al 1991;Steinberg, 1997;Holvoet & Collen, 1998). Several studies have investigated the effect of altering the fat composition of the diet on LDL oxidation.…”

Section: Discussionmentioning

confidence: 99%

Eicosapentaenoic acid and docosahexaenoic acid from fish oils: differential associations with lipid responses

Leigh-Firbank

A.M.*²,

Leake

et al. 2002

British Journal of Nutrition

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Fish-oil supplementation can reduce circulating triacylglycerol (TG) levels and cardiovascular risk. This study aimed to assess independent associations between changes in platelet eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and fasting and postprandial (PP) lipoprotein concentrations and LDL oxidation status, following fish-oil intervention. Fiftyfive mildly hypertriacylglycerolaemic (TG 1·5-4·0 mmol/l) men completed a double-blind placebo controlled cross over study, where individuals consumed 6 g fish oil (3 g EPA þ DHA) or 6 g olive oil (placebo)/d for two 6-week intervention periods, with a 12-week wash-out period in between. Fish-oil intervention resulted in a significant increase in the platelet phospholipid EPA (+491 %, P,0·001) and DHA (+44 %, P,0·001) content and a significant decrease in the arachidonic acid (210 %, P,0·001) and g-linolenic acid (224 %, P,0·001) levels. A 30 % increase in ex vivo LDL oxidation (P,0·001) was observed. In addition, fish oil resulted in a significant decrease in fasting and PP TG levels (P,0·001), PP non-esterified fatty acid (NEFA) levels, and in the percentage LDL as LDL-3 (P¼0·040), and an increase in LDLcholesterol (P¼0·027). In multivariate analysis, changes in platelet phospholipid DHA emerged as being independently associated with the rise in LDL-cholesterol, accounting for 16 % of the variability in this outcome measure (P¼0·030). In contrast, increases in platelet EPA were independently associated with the reductions in fasting (P¼0·046) and PP TG (P¼0·023), and PP NEFA (P¼0·015), explaining 15 -20 % and 25 % of the variability in response respectively. Increases in platelet EPA þ DHA were independently and positively associated with the increase in LDL oxidation (P¼0·011). EPA and DHA may have differential effects on plasma lipids in mildly hypertriacylglycerolaemic men.

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Section: Discussionmentioning

confidence: 99%

Eicosapentaenoic acid and docosahexaenoic acid from fish oils: differential associations with lipid responses

Leigh-Firbank

A.M.*²,

Leake

et al. 2002

British Journal of Nutrition

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“…Agarose gel electrophoresis revealed advanced oxidization of LDL in the SZ group by increasing the relative mobility of the LDL fraction [6,18,19,41,61], though a clear difference between the plasma ox-LDL concentrations in the SZ group and those in the CB group could not be detected by dot blot analysis (data not shown). The susceptibility of LDL to oxidation has been reported to have a significant relation to progression of atherosclerosis in humans [3,40], and more attention has been paid to the oxidizability of LDL from the aspect of local oxidization in the arterial walls [5,46,49,54]. Therefore, when there would be excessive LDL in blood, LDL might stay longer in the arterial wall, where it could be oxidatively modified because of its high susceptibility to oxidization.…”

Section: Discussionmentioning

confidence: 99%

APA Hamster Model for Diabetic Atherosclerosis. 2. Analysis of Lipids and Lipoproteins.

et al. 2000

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Syrian hamsters of the APA strain (APA hamsters) have recently been shown to have atheromatous lesions in the aortic arches under diabetic condition induced by a single injection of streptozotocin (SZ). In that model, fatty streaks, which are the initial lesions of atherogenesis, develop by 6 weeks after the injection (WAI). In this study, we evaluated plasma lipid concentrations and lipoprotein profiles in diabetic APA hamsters at 6 WAI to reveal the early stage of atherogenesis clinicopathologically. As a result, by biochemical analysis, hyperglycemic APA hamsters showed signs of hypercholesterolemia and hypertriglyceridemia. Low-density lipoprotein (LDL) cholesterol significantly increased, but high-density lipoprotein (HDL) cholesterol significantly decreased. Agarose gel electrophoresis showed an obvious increase in the fractions of chylomicron, LDL and abnormal lipoprotein. Plasma LDL in diabetic animals was in a state more susceptible to oxidization. In addition, a significant increase in glycated LDL was also found in the diabetic animals by enzyme linked immunosorbent assay (ELISA). Moreover, lipid peroxidation product (4-hydroxynonenal (4 HNE))-adducted proteins and advanced glycation end-products (AGE) were immunohistochemically detected in the foam cells of the fatty streaks. These results revealed that diabetic APA hamsters had hyperlipidemia characterized by increases in chylomicron, LDL and abnormal lipoprotein, and suggested that oxidized LDL and/or glycated LDL might be actively uptaken by macrophages and play an important role in the initial stage of atherogenesis.

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“…Increased LDL susceptibility to oxidation and elevated levels of lipid oxidation products were found in patients with CHD (Rengstrom et al 1992, Andrews et al 1995, Iribarren et al 1997). On the other hand, inverse relationship between plasma antioxidants such as vitamin E and atherosclerotic disease was found in several clinical studies (Stampfer et al 1993, Knekt et al 1994, Iribarren et al 1997.…”

Section: Introductionmentioning

confidence: 95%

Increased oxidative stress in subjects exposed to carbon disulfide (CS 2 ) - an occupational coronary risk factor

Wrońska-Nofer

Chojnowska-Jezierska²,

Nofer

et al. 2002

Archives of Toxicology

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There is considerable epidemiological evidence that workers exposed to carbon disulfide (CS2) develop premature atherosclerosis leading to increased rates of coronary heart disease (CHD), but mechanisms underlying this association remain obscure. The present study documents that occupational exposure to CS2 modifies the oxidative status of plasma, which is a major determinant of the susceptibility to atherosclerosis. Concentrations of thiobarbituric reactive substances (TBARS), which reflect lipid peroxidation processes in plasma, were determined in 29 men who were exposed to CS2 for more than 20 years, in 24 patients with peripheral atherosclerosis, and in 30 unexposed, healthy control subjects. TBARS concentrations were significantly increased both in CS2-exposed subjects and in patients with peripheral atherosclerosis. Subjects in both groups presented also with decreased levels of plasma alpha-tocopherol, a major plasma antioxidant. In addition, decreased activities of two enzymatic antioxidants, glutathion peroxidase and catalase, were noted both in CS2-exposed subjects and patients with peripheral atherosclerosis. Finally, LDL isolated from both groups showed increased susceptibility to transition metal-induced oxidation in vitro. It is concluded, that occupational exposure to CS2 produces oxidative stress in plasma. This may favor the development of atherosclerosis and increase the incidence of CHD in workers exposed to CS2.

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Oxidisability of low density lipoproteins in patients with carotid or femoral artery atherosclerosis

Cited by 49 publications

References 10 publications

Eicosapentaenoic acid and docosahexaenoic acid from fish oils: differential associations with lipid responses

Eicosapentaenoic acid and docosahexaenoic acid from fish oils: differential associations with lipid responses

APA Hamster Model for Diabetic Atherosclerosis. 2. Analysis of Lipids and Lipoproteins.

Increased oxidative stress in subjects exposed to carbon disulfide (CS 2 ) - an occupational coronary risk factor

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