Oxidative Stress, Vitamin E, and Antioxidant Capacity in Knee Osteoarthritis

Suantawee, Tanyawan; Tantavisut, Saran; Tanavalee, Aree; Yuktanandana, Pongsak; Anomasiri, Wilai; Deepaisarnsakul, Benjamad; Honsawek, Sittisak

doi:10.7860/jcdr/2013/5802.3333

“…Oxidative stress shows a potential role on the pathogenesis of OA. In subjects suffering from OA there is a reduction in antioxidants [47]. Proinflammatory cytokines (IL-1β, IL-6, and TNF-α), chemokines (MIP-Iα and MIP-2), and NO also play an important function in the pathogenesis of joint damage [48][49][50].…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of New Carnosine-Hyaluronic Acid Conjugate on the Inflammation and Cartilage Degradation in the Experimental Model of Osteoarthritis

Siracusa

¹

,

Impellizzeri

²

,

Cordaro

³

et al. 2020

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Osteoarthritis (OA) is a disease that currently has no cure. There are numerous studies showing that carnosine and hyaluronic acid (HA) have a positive pharmacological action during joint inflammation. For this reason, the goal of this research was to discover the protective effect of a new carnosine conjugate with hyaluronic acid (FidHycarn) on the inflammatory response and on the cartilage degradation in an in vivo experimental model of OA. This model was induced by a single intra-articular (i.ar.) injection of 25 µL of normal saline with 1 mg of monosodium iodoacetate solution (MIA) in the knee joint of rats. MIA injection caused histological alterations and degradation of the cartilage, as well as behavioral changes. Oral treatment with FidHycarn ameliorated the macroscopic signs, improved thermal hyperalgesia and the weight distribution of the hind paw, and decreased histological and radiographic alterations. The oxidative damage was analyzed by evaluating the levels of nitrotyrosine and inducible nitric oxide synthase (iNOS) that were significantly reduced in FidHycarn rats. Moreover, the levels of pro-inflammatory cytokines and chemokines were also significantly reduced by FidHycarn. Therefore, for the first time, the effectiveness of oral administration of FidHycarn has been demonstrated in an osteoarthritis model. In conclusion, the new FidHycarn could represent an interesting therapeutic strategy to combat osteoarthritis. affects the joints that support most of the weight (such as the knees and feet) and the joints we use the most (e.g., hand joints). In a healthy joint, the cartilage shelters the surface of the bones and supports the bones to move liberally against each other. When a joint develops OA, part of the cartilage tapers and the surface becomes coarser. This means the articulation does not move as easily as it should. When the cartilage becomes worn out or damaged, all the tissues within the articulation become more active than usual as the body attempts to repair the damage. However, the reparation processes do not always operate well, and thus, modifications to the joint structure can occasionally cause or contribute to symptoms such as swelling, pain, and ultimately, disability.One of the leading characteristics of OA is collagen deterioration, as suggested by augmented tissue swelling and the loss of proteoglycans [1]. Both collagen destruction and the loss of proteoglycans unfavorably affect the mechanical properties of cartilage. Chondrocytes respond to tissue injury by increasing collagen and proteoglycan synthesis in an attempt to repair the tissue [2]. If repair fails, the damage will progress to articular cartilage degeneration. It is still not clear exactly what causes osteoarthritis. We do know that it is not simply 'wear and tear' and that the danger of developing OA depends on a number of factors.Furthermore, these degradation processes are thought to be largely elicited through excess production of pro-inflammatory and catabolic mediators. Among them, interleukin-1β (IL-1β) has...

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“…Malonaldehyde is the end product of the peroxidation process, nitrite is the final agent of RNS activity, both of which have been identified in high amounts in the plasma and synovial fluid of patients with OA [17,18]. Additionally, a decrease in the function of the body's antioxidant systems has been demonstrated in patients with OA [19]. Nitric oxide can also react with ROS producing peroxynitrite, which induces chondrocyte apoptosis by activating the caspase pathway and nuclear factor kappa B (NF-κB) with the perpetuation of the intra-articular inflammatory process [20].…”

Section: Cartilage Oxidationmentioning

confidence: 99%

Apitherapy for Osteoarthritis: Perspectives from Basic Research

Jagua-Gualdrón

¹

,

Peña-Latorre

²

,

Fernadez-Bernal

³

2020

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Osteoarthritis is one of the most common rheumatic disease in the world and one of the leading causes of disability in the elderly. There is still no curative management for the disease, so the search for new therapeutic alternatives continues. Apitherapy is a therapeutic tool based on the use of beehive products used since ancient times and, at present, their mechanism of action begins to be known. Many of the mechanisms of action of the beehive products are useful for chronic articular pathophysiological processes such as those described in osteoarthritis. This article presents a review of the current state of understanding of the mechanisms through which bee venom, propolis, honey, pollen, and royal jelly may act on osteoarthritis. © 2020 S. Karger AG, Basel Apitherapie bei Osteoarthritis: Perspektiven aus der GrundlagenforschungSchlüsselwörter Apitherapie · Osteoarthritis · Bienengift · Bienenprodukte · Komplementär-und Alternativmedizin ZusammenfassungOsteoarthritis ist eine der weltweit häufigsten rheumatischen Erkrankungen und eine der führenden Ursachen für körperliche Beeinträchtigung im Alter. Eine kurative Behandlungsoption steht bis heute nicht zur Verfügung, daher wird weiter nach therapeutischen Alternativen gesucht. Die Apitherapie ist ein therapeutisches Instrument, das auf der Anwendung von Bienenprodukten beruht, die seit dem Altertum verwendet werden und deren Wirkmechanismen wir gerade beginnen zu verstehen. Viele dieser Mechanismen, über die die Bienenprodukte ihre Wirkung entfalten, spielen eine nützliche Rolle in pathophysiologischen Prozessen von chronischen Gelenkerkrankungen wie zum Beispiel der Osteoarthritis. Dieser Artikel gibt einen Überblick über den aktuellen Kenntnisstand der Mechanismen, über die Bienengift, Propolis, Honig, Pollen und Gelée royale bei Osteoarthritis wirken könnten.

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“…Oxidative stress shows a potential role on the pathogenesis of OA. In fact, in subjects suffering from OA there is a reduction in antioxidants [46]. Also proinflammatory cytokines (IL-1β, IL-6, and TNF-α), chemokines (MIP-Iα and MIP-2) and NO play an important function in the pathogenesis of joint damage [47][48][49].…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of New Formulation of Carnosine+Hyaluronic Acid on the Inflammation and Cartilage Degradation in the Experimental Model of Osteoarthritis

Siracusa¹,

Impellizzeri²,

Cordaro³

et al. 2020

Preprint

1

0

View full text Add to dashboard Cite

Osteoarthritis (OA) is a disease that currently has no cure. There are numerous studies showing that carnosine and hyaluronic acid (HA) have a positive pharmacological action during joint inflammation. For this reason, the goal of this research was to discover the protective effect of a new HA+Carnosine formulation (FidHycarn) on the inflammatory response and on the cartilage degradation in in vivo experimental model of OA. This model was induced by a single intra-articular (i.ar.) injection of 25µl normal saline having 1mg of monosodium iodoacetate solution (MIA) in the knee joint. MIA injection caused histological alterations and degradation of cartilage as well as behavioral changes. Oral treatment with FidHycarn ameliorated the macroscopic signs, improved thermal hyperalgesia and weight distribution of hind paw as well as decreased histological and radiographic alterations. The oxidative damage was analyzed by evaluating the levels of nitrotyrosine and inducible nitric oxide synthase (iNOS) that were significantly reduced in FidHycarn rats. Moreover, the levels of pro-inflammatory cytokines and chemokines were also significantly reduced by FidHycarn. However, interestingly, in more cases, the effects of FidHycarn were not statistically different to Naproxen used as positive control. Thus, the new formulation containing Carnosine and HA could represent an interesting therapeutic strategy to combat osteoarthritis.

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Oxidative Stress, Vitamin E, and Antioxidant Capacity in Knee Osteoarthritis

Abstract: The increased plasma levels of nitrite and MDA and the decreased plasma levels of vitamin E, TEAC, and FRAP indicated that oxidative stress was present in OA patients. These findings suggest that oxidative stress plays a potential role in pathophysiology of knee osteoarthritis.

Cited by 39 publications

References 26 publications

The Protective Effect of New Carnosine-Hyaluronic Acid Conjugate on the Inflammation and Cartilage Degradation in the Experimental Model of Osteoarthritis

The Protective Effect of New Carnosine-Hyaluronic Acid Conjugate on the Inflammation and Cartilage Degradation in the Experimental Model of Osteoarthritis

Apitherapy for Osteoarthritis: Perspectives from Basic Research

Protective Effect of New Formulation of Carnosine+Hyaluronic Acid on the Inflammation and Cartilage Degradation in the Experimental Model of Osteoarthritis

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