Oxidative Stress Status in Rats After Intermittent Exposure to Hypobaric Hypoxia

Esteva, Santiago; Pedret, Rafel; Fort, Núria; Torrella, Joan Ramón; Pagés, Teresa; Viscor, Ginés

doi:10.1016/j.wem.2010.09.004

Cited by 26 publications

(14 citation statements)

References 55 publications

(69 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the contrary, Esteva et al (2010) did not find significant differences in the oxidative stress parameters in animals submitted to intermittent hypobaric hypoxia (5000 m). Although we found a significant increment in plasma protein oxidation after 21 days of hypoxic exposure, administration of vitamin C did not prevent it.…”

contrasting

confidence: 61%

Vitamin C Supplementation Does not Improve Hypoxia-Induced Erythropoiesis

Martínez-Bello

Sanchís-Gomar

Martinez‐Bello

et al. 2012

High Altitude Medicine & Biology

View full text Add to dashboard Cite

a. Vitamin C Supplementation Does Not Improve Hypoxia-Induced Erythropoiesis. High Alt Med Biol 13:269-274, 2012.-Hypoxia induces reactive oxygen species production. Supplements with antioxidant mixtures can compensate for the decline in red cell membrane stability following intermittent hypobaric hypoxia by decreasing protein and lipid oxidation. We aimed to determine whether supplementation with vitamin C is implicated in the regulation of erythropoiesis and in the oxygen-carrying capacity of the blood, and also whether antioxidant supplementation prevents the oxidative stress associated to intermittent hypoxia. Twentyfour male Wistar rats were randomly divided into four experimental groups: normoxia control (n = 6), normoxia + vitamin C (n = 6), hypoxia control (12 h pO 2 12%/12 h pO 2 21%) (n = 6), and hypoxia + vitamin C (n = 6). Animals were supplemented with vitamin C at a dose of 250 mg$kg -1 $day -1 for 21 days. Red blood cell count, hemoglobin, hematocrit, reticulocytes, erythropoietin, and oxidative stress parameters such as malondialdehyde and protein oxidation in plasma were analyzed at two different time points: basal sample (day zero) and final sample (day 21). Similar RBC, Hb, Hct, and Epo increments were observed in both hypoxic groups regardless of the vitamin C supplementation. There was no change on MDA levels after intermittent hypoxic exposure in any experimental group. However, we found an increase in plasma protein oxidation in both hypoxic groups. Vitamin C does not affect erythropoiesis and protein oxidation in rats submitted to intermittent hypoxic exposure.

show abstract

contrasting

confidence: 61%

Vitamin C Supplementation Does not Improve Hypoxia-Induced Erythropoiesis

Martínez-Bello

Sanchís-Gomar

Martinez‐Bello

et al. 2012

High Altitude Medicine & Biology

View full text Add to dashboard Cite

show abstract

“…Under hypoxia and during exercise, the increased level of ROS induces an increase of erythrocyte mass, contributing to the organism's adaptation to such conditions. However, excessive amounts of ROS as a result of an imbalance in the pro-and antioxidant systems induce oxidative damage in cells and tissues, which is termed oxidative stress [2]. Erythrocytes, the unique carriers of oxygen, are constantly exposed to ROS due to their function in the organism.…”

Section: Introductionmentioning

confidence: 99%

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Olchowik

Lotkowski

Mavlyanov

et al. 2012

Cellular and Molecular Biology Letters

View full text Add to dashboard Cite

Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5-50 µg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 µM ONOO -or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.

show abstract

“…Hypoxia exposure has also been combined with normal athletic training in a variety of ways [48]. More specifically, IHH exposure has been shown to be an efficient stimulus for eliciting different adaptive responses so as to improve peripheral oxygen supply [49,50]. Serebrovskaya et al reported similar results with a normobaric hypoxia exposure programme that was able to enhance innate immunity by mobilizing progenitor cells, activating neutrophils and by increasing circulating complement and immunoglobulins [33].…”

Section: Discussionmentioning

confidence: 99%

Combined intermittent hypobaric hypoxia and muscle electro-stimulation: a method to increase circulating progenitor cell concentration?

Corral

Javierre

Blasi

et al. 2014

Journal of Translational Medicine

Self Cite

View full text Add to dashboard Cite

BackgroundOur goal was to test whether short-term intermittent hypobaric hypoxia (IHH) at a level well tolerated by healthy humans could, in combination with muscle electro-stimulation (ME), mobilize circulating progenitor cells (CPC) and increase their concentration in peripheral circulation.MethodsNine healthy male subjects were subjected, as the active group (HME), to a protocol involving IHH plus ME. IHH exposure consisted of four, three-hour sessions at a barometric pressure of 540 hPa (equivalent to an altitude of 5000 m). These sessions took place on four consecutive days. ME was applied in two separate 20-minute periods during each IHH session. Blood samples were obtained from an antecubital vein on three consecutive days immediately before the experiment, and then 24 h, 48 h, 4 days, 7 days and 14 days after the last day of hypoxic exposure. Four months later a control study was carried out involving seven of the original subjects (CG), who underwent the same protocol of blood samples but without receiving any special stimulus.ResultsIn comparison with the CG the HME group showed only a non-significant increase in the number of CPC CD34+ cells on the fourth day after the combined IHH and ME treatment.ConclusionCPC levels oscillated across the study period and provide no firm evidence to support an increased CPC count after IHH plus ME, although it is not possible to know if this slight increase observed is physiologically relevant. Further studies are required to understand CPC dynamics and the physiology and physiopathology of the hypoxic stimulus.

show abstract

Oxidative Stress Status in Rats After Intermittent Exposure to Hypobaric Hypoxia

Abstract: The absence of significant differences between groups indicates that our IHH program has little impact on the general redox status, even in the laboratory rat, which is more sensitive to hypoxia than humans. We conclude that IHH does not increase oxidative stress.

Cited by 26 publications

References 55 publications

Vitamin C Supplementation Does not Improve Hypoxia-Induced Erythropoiesis

Vitamin C Supplementation Does not Improve Hypoxia-Induced Erythropoiesis

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Combined intermittent hypobaric hypoxia and muscle electro-stimulation: a method to increase circulating progenitor cell concentration?

Contact Info

Product

Resources

About