Oxidative stress responses during cassava post-harvest physiological deterioration

Reilly, Kim; Gomez-Vasquez, R.; Buschmann, Holger; Tohmé, Joe; Beeching, John R.

doi:10.1023/b:plan.0000019076.76614.88

Cited by 93 publications

(142 citation statements)

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“…KIMURA et al (2007) also observed a decrease of the concentration of carotenoids during storage. The degradation of carotenoids may have occurred due to the oxidative stress suffered by cassava as a function of tissue exposure to oxygen, due to cut into stick form (REILLY et al, 2003;CHAVEZ et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage

et al. 2014

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Section: Resultsmentioning

confidence: 99%

Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage

et al. 2014

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“…Previous studies have shown that mechanical injury of cassava storage roots triggers cyanogenesis (McMahon et al, 1995) and an associated burst of acetone from cyanohydrins detected throughout the storage root (Iyer et al, 2010), followed by the production of ROS (Reilly et al, 2004). Cyanogenesis in cassava is induced during rupture of the vacuole, where linamarin is stored, followed by the production of cyanide in a two-step process initially catalyzed by the cell wall-localized enzyme linamarase and in leaves accelerated by hydroxynitrile lyase activity (Mkpong et al, 1990;White et al, 1998).…”

Section: The Oxidative Burst In Damaged Cassava Roots Is Cyanogen Indmentioning

confidence: 99%

“…However, the short shelf life of the roots (only 2-3 d) limits cassava's economic and industrial potential. Harvested cassava roots undergo rapid postharvest physiological deterioration (PPD), which reduces their quality for market and consumption (Booth, 1976;Wenham, 1995;Buschmann et al, 2000;Reilly et al, 2001Reilly et al, , 2004Westby, 2002;Iyer et al, 2010). Therefore, cassava processing facilities, if any, must be at or near the site of production to reduce postharvest losses.…”

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confidence: 99%

“…Several studies have been carried out to understand the biochemistry of PPD (Buschmann et al, 2000;Huang et al, 2001;Reilly et al, 2001Reilly et al, , 2004Iyer et al, 2010). These studies have placed reactive oxygen species (ROS) production as one of the earliest events in the deterioration process.…”

mentioning

confidence: 99%

“…In cassava roots, an oxidative burst occurs within 15 min of harvest (Reilly et al, 2004). Other early events include increased activity of enzymes that modulate ROS levels, such as catalase, peroxidase, and superoxide dismutase (Buschmann et al, 2000;Reilly et al, 2001;Iyer et al, 2010).…”

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Extending Cassava Root Shelf Life via Reduction of Reactive Oxygen Species Production

Zidenga

Leyva-Guerrero²,

Moon³

et al. 2012

Plant Physiology

138

168

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One of the major constraints facing the large-scale production of cassava (Manihot esculenta) roots is the rapid postharvest physiological deterioration (PPD) that occurs within 72 h following harvest. One of the earliest recognized biochemical events during the initiation of PPD is a rapid burst of reactive oxygen species (ROS) accumulation. We have investigated the source of this oxidative burst to identify possible strategies to limit its extent and to extend cassava root shelf life. We provide evidence for a causal link between cyanogenesis and the onset of the oxidative burst that triggers PPD. By measuring ROS accumulation in transgenic low-cyanogen plants with and without cyanide complementation, we show that PPD is cyanide dependent, presumably resulting from a cyanide-dependent inhibition of respiration. To reduce cyanide-dependent ROS production in cassava root mitochondria, we generated transgenic plants expressing a codon-optimized Arabidopsis (Arabidopsis thaliana) mitochondrial alternative oxidase gene (AOX1A). Unlike cytochrome c oxidase, AOX is cyanide insensitive. Transgenic plants overexpressing AOX exhibited over a 10-fold reduction in ROS accumulation compared with wild-type plants. The reduction in ROS accumulation was associated with a delayed onset of PPD by 14 to 21 d after harvest of greenhouse-grown plants. The delay in PPD in transgenic plants was also observed under field conditions, but with a root biomass yield loss in the highest AOX-expressing lines. These data reveal a mechanism for PPD in cassava based on cyanideinduced oxidative stress as well as PPD control strategies involving inhibition of ROS production or its sequestration.

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Cassava Genetic Improvement

Ceballos¹,

Fregene²,

Carlos³

et al. 2007

Breeding Major Food Staples

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PREFACEThis Handbook is the result of a combined effort by several current and previous cassava researchers at CIAT to review and summarize the most important results of cassava research during the past 40 years. Most of the chapters are based on the various presentations during the Regional Cassava Training Course, held in Thailand from October 6 to 17, 2008. This course was organized upon the realization that many people that were actively involved with cassava research in the 1970s and 80s, both at CIAT and in national programs, have now retired or will soon do so, and that a whole new generation of cassava researchers are currently being hired to take their place.As cassava is now becoming a very important, and mostly industrial, crop in Asia, there are many new opportunities, but also a host of new problems and challenges. These include the appearance in Asia of new cassava diseases and pests; the decreasing availability and increasing cost of rural labor, resulting in the need for partial or complete mechanization of cassava production; the rapidly increasing demand for cassava roots for production of food, feed and fuel, and the unavailability in many countries of new land for any expansion of cassava area, thus requiring a rapid increase in cassava yields to increase supplies. This requires a renewed focus on cassava research for the development of new higher-yielding varieties and more sustainable production practices.While many cassava researchers in national programs in Asia received individual or group training at CIAT-Colombia during the 1970s and 80s, this training was greatly reduced during the following two decades due to funding limitations. Thus, the objective of the Regional Cassava Training Course in 2008 was to provide a new training opportunity for young scientists in Asian countries, and to hand over the knowledge and experience of the older cassava researchers, mostly from CIAT, to a new generation that will have to face the new challenges. Thus, the course was taught mostly by current or already retired CIAT cassava researchers, while the 60-plus participants of the course included cassava researchers from Cambodia, China, East Timor, India, Indonesia, Lao PDR, Malaysia, Myanmar, the Philippines, Thailand and Vietnam. The training course not only provided knowledge -from physiology and biotechnology to animal feeding and production of fuelethanol -but also an opportunity for people from different institutions and countries to get to know each other, which will greatly facilitate future collaboration.Course participants returned home with a CD containing the PowerPoint presentations of the course. However, it was felt that a more comprehensive review of all the topics covered was warranted, as this would provide more in-depth knowledge for those working in the various specialized fields. Most of this information is available in many refereed journals, in workshop proceedings and old CIAT annual reports, but many of these are now out of print or otherwise difficult to obtain. Thus, the Cas...

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Oxidative stress responses during cassava post-harvest physiological deterioration

Cited by 93 publications

References 47 publications

Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage

Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage

Extending Cassava Root Shelf Life via Reduction of Reactive Oxygen Species Production

Cassava Genetic Improvement

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