Oxidative stress response as biomarker of exposure of a freshwater invertebrate model organism (Unio mancus Lamarck, 1819) to antifouling copper pyrithione

Abstract

Sublethal effects of copper pyrithione (CuPT) on an invertebrate model organism, freshwater mussel (Unio mancus), were assessed using oxidative stress parameters and histopathology. The environmentally relevant concentration of 5 ?g/l copper pyrithione was applied as a 96 h semi-static exposure. MDA levels in digestive gland (DG) significantly increased, compared to a control group (p < 0.001). Gill MDA levels also increased, but not significantly (p > 0.05). GSH level in DG increased significantly (p < 0.05), and gill tissue levels also increased but not significantly (p > 0.05). Levels of SOD, GPx activities, and AOPP levels did not change significantly (p > 0.05). The most prominent histopathological alterations in the gills were haemocyte infiltration, lipofuscin aggregates and lamellar deformations with tubule degeneration and accumulated lipofuscin aggregates in DG tubules. CuPT impacts antioxidant defense systems even during sublethal exposure of Unio mancus as the freshwater model organism.

Ecotoxicity of Copper(I) Chloride in Grooved Carpet Shell (Ruditapes decussatus)

“…In this study, the digestive gland was the most involved tissue in the modulation of the total glutathione levels. Recent data showed that GSH levels increased significantly in the digestive glands of freshwater mussel ( Unio mancus ) exposed to antifouling copper pyrithione, while thiol levels were unchanged in the gills [ 67 ]. These findings are in agreement with previous research in which the key role of the digestive gland in metal bioaccumulation and detoxification was discussed [ 68 ].…”

Evaluation of freshwater heavy metals accumulation effect on oxidative stress, Metallothionein biosynthesis and histopathology of Procambarus clarkii (Girard,1985) collected from three locations in the Delta region, Egypt

“…The findings of this study demonstrated that the hepatopancreas of crayfish suffered substantial structural damage as a result of accumulating distribution levels. Non-essential elements have been shown in other investigations [ 26 , 48 ] to accumulate in crayfish tissues as environmental concentration and exposure time increase.…”

“…Also, our results came along with results of Wang et al [ 25 ], who recorded a significant increase in SOD and GST activity after exposure of freshwater crab Sinopotamon henanense ’s gills to Cd. Furthermore, an increase in SOD level in both gills and hepatopancreas of Unio macus was noticed after exposure to Cu [ 26 ]. The studies indicated that gill and hepatopancreas tissues are the most sensitive tissues exposed to toxicants.…”