Oxidative stress preconditioning of mouse perivascular myogenic progenitors selects a subpopulation of cells with a distinct survival advantage in vitro and in vivo

Abstract

Cell engraftment, survival and integration during transplantation procedures represent the crux of cell-based therapies. Thus, there have been many studies focused on improving cell viability upon implantation. We used severe oxidative stress to select for a mouse mesoangioblast subpopulation in vitro and found that this subpopulation retained self-renewal and myogenic differentiation capacities while notably enhancing cell survival, proliferation and migration relative to unselected cells. Additionally, this subpopulation of cells presented different resistance and recovery properties upon oxidative stress treatment, demonstrating select advantages over parental mesoangioblasts in our experimental analysis. Specifically, the cells were resistant to oxidative environments, demonstrating survival, continuous self-renewal and improved migration capability. The primary outcome of the selected cells was determined in in vivo experiments in which immunocompromised dystrophic mice were injected intramuscularly in the tibialis anterior with selected or non-selected mesoangioblasts. Resistant mesoangioblasts exhibited markedly enhanced survival and integration into the host skeletal muscle, accounting for a more than 70% increase in engraftment compared with that of the unselected mesoangioblast cell population and leading to remarkable muscle recovery. Thus, the positive effects of sorting on mesoangioblast cell behaviour in vitro and in vivo suggest that a selection step involving oxidative stress preconditioning may provide a novel methodology to select for resistant cells for use in regenerative tissue applications to prevent high mortality rates upon transplantation.

Expression Profiling of Extracellular Matrix Genes Reveals Global and Entity-Specific Characteristics in Adenoid Cystic, Mucoepidermoid and Salivary Duct Carcinomas

“…In summary, we revealed an overexpression of ECM components, which are associated with enhanced migratory capacities of tumour cells ( TNC [ 76 , 77 ], FN1 [ 78 ]), resistance to chemotherapy ( COL11A1 [ 43 , 75 ], SPP1 [ 79 ]) and adverse outcome ( COL11A1 [ 42 ], TNC [ 80 ], FN1 [ 78 ]) in other primaries. The functional role of these molecules and their potential as therapeutic targets are currently under intense investigation [ 65 , 66 , 75 , 79 , 81 , 82 ]. Hence, as new therapeutic approaches for SGC are needed, our findings are of high translational relevance.…”

Identification of hsa_circ_0039053 as an up-regulated and oncogenic circRNA in hepatocellular carcinoma via the miR-637-mediated USP21 activation

“…Herein, we revealed that hsa_circ_0039053 could serve as the ceRNA of miR-637 to increase USP21 expression in HCC. Previous studies demonstrated that miR-637 exerted the tumor-inhibitory roles in diverse human tumors, such as cervical cancer, colorectal cancer, and gastric cancer 23 - 25 . In consistence of previous studies, we also observed that miR-637 was reduced in HCC and the elevation of miR-637 was linked to poor disease outcomes.…”

Anti-Inflammatory Activity and ROS Regulation Effect of Sinapaldehyde in LPS-Stimulated RAW 264.7 Macrophages

“…Lipopolysaccharide (LPS) is widely localized in the outer cell wall of Gram-negative bacteria. Thus, LPS elicits a host inflammatory response that results in increased production of chemokines, cytokines, and pro-inflammatory mediators by the immune system [ 10 ]. Macrophages exposed to LPS during microorganism infections stimulate the immune system to produce cytokines and chemokines, followed by inflammatory events.…”