Oxidative Stress Mediates Anxiety-Like Behavior Induced by High Caffeine Intake in Zebrafish: Protective Effect of Alpha-Tocopherol

Carvalho, Tayana Silva de; Cardoso, Patrick Bruno; Santos-Silva, Mateus; Lima-Bastos, Sávio; Luz, Waldo Lucas; Assad, Nadyme; Kauffmann, Nayara; Passos, Adelaide da Conceição Fonseca; Brasil, Alódia; Bahia, Carlomagno Pacheco; Moraes, Suellen Alessandra Soares de; Gouvêia, Amauri; Batista, Evander de Jesus Oliveira; Oliveira, Karen Renata Matos; Herculano, Anderson Manoel

doi:10.1155/2019/8419810

Cited by 36 publications

(19 citation statements)

References 36 publications

(41 reference statements)

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“…The results were consistent with some studies. For example, a study found that the treatment of caffeine could increase the level of MDA in the zebrafish brain (de Carvalho et al., 2019). Several studies showed that ellagic acid and gallic acid could ameliorate liver steatosis in mice (Sousa et al., 2020; Zhang et al., 2019).…”

Section: Resultsmentioning

confidence: 99%

Effects of several tea extracts on nonalcoholic fatty liver disease in mice fed with a high‐fat diet

Mao

Meng

et al. 2021

Food Science & Nutrition

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Nonalcoholic fatty liver disease (NAFLD) is considered as a severe threat to human health. It has been reported that tea has abundant bioactive compounds and beneficial effects. In our study, the effects of 12 tea extracts on NAFLD were assessed and compared at the dose of 200 mg/kg body weight in mice fed with a high‐fat diet (HFD) for 15 weeks. Enshi Yulu Tea, Fenghuang Narcissus Tea, and Yihong Tea showed strong effects in suppressing the accumulation of epididymal and perirenal adipose tissue as well as the increases of body weight and liver weight. The histopathological analysis revealed that hepatic steatosis and adipocyte hypertrophy induced by a HFD could be ameliorated by tea supplementation. In addition, Enshi Yulu Tea and Qing Brick Tea exerted more remarkable functions on decreasing the level of serum triglyceride and preventing hepatic fat accumulation, respectively. Furthermore, Fenghuang Narcissus Tea, Enshi Yulu Tea, and Qing Brick Tea could reverse the abnormal change in the levels of glutathione and superoxide dismutase. Moreover, 13 phytoconstituents were detected and quantified in these teas with high‐performance liquid chromatography (HPLC) method. The correlation analysis demonstrated that gallic acid might decrease MDA level, and the reduction of liver weight might be attributed to ellagic acid. However, it should be paid attention to some teas that showed hepatotoxicity with elevated levels of aspartate transaminase and alanine aminotransferase. Several teas showed strong effects in the prevention of NAFLD, which could be developed into functional foods against NAFLD.

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Section: Resultsmentioning

confidence: 99%

Effects of several tea extracts on nonalcoholic fatty liver disease in mice fed with a high‐fat diet

Mao

Meng

et al. 2021

Food Science & Nutrition

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“…The flowers of the plant contain quercetin, and isoquercetin, while stem contains beta-sitosterol. Alpha-tocopherol exerted a protective effect against high-caffeine induced anxiety effects in Zebrafish (34). Quercetin is evident to reduce cortical GABAergic transmission (35) and exert an anxiolytic effect on experimental animals (36).…”

Section: Metabolitesmentioning

confidence: 94%

Anxiolytic-like effects of Moringa oleifera in Swiss mice

Islam

Martins

Imran

et al. 2020

Cell Mol Biol (Noisy-le-grand)

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Moringa oleifera is evident to act against many neurological diseases, including muscle spasm, epilepsy, nervousness, fatigue, memory impairment, convulsion, and epilepsy. Anxiety represents the most common and disabling psychiatric condition, being often associated with depressive symptoms. This study investigated the anxiolytic-like effects of crude organic fractions of M. oleifera leaves in different behavioral paradigms that evaluate anxiety in mice. To this end, mice were administered with crude extracts (500 mg/kg, p.o.) and/or diazepam (2 mg/kg, p.o.), and submitted to behavioral tests. In the open-field test, the number of square field cross, grooming and rearing were calculated, while in light-dark and swing test were, respectively, the time spent in dark portion and number of swings. Each test was performed for 3 min. M. oleifera leaf methanol and n-hexane extracts elicited an anxiolytic-like effect observed by increased total time in the center and decreased number of rearings and groomings responses in the open field and swing tests, and residence in the dark portion in the light-dark box, similar to the diazepam group. A moderate anxiolytic effect was observed in the aqueous fraction group, while insignificant effects were recorded in the ethyl acetate fraction group in all test paradigms. In addition, both extracts potentiate the calming effects of diazepam in experimental animals. Preliminary phytochemical reports suggest that M. oleifera contains alkaloids, flavonoids, phenols, steroids, glycosides, saponins, tannin, terpenes, and gums. Of note, the results expand the understanding of M. oleifera effects in central nervous system and suggest that plant metabolites may be helpful for anxiety-related disorders management.

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“…Zebrafish were cold-anesthetized and injected intra-abdominally with either saline solution (SAL, 0.9%) or 1 mg/Kg ACEA dissolved in 0.005% DMSO. Drug dilution and treatments were performed 30 min before the behavioral experiments to assure drug distribution and reach in zebrafish brain as performed by de Carvalho et al ( 2019 ) and Assad et al ( 2020 ). The fish were then transferred to individual plastic aquaria, in which they were kept for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…The product of lipid peroxidation was determined in the supernatant using 20 mM of pH 7.4 Tris-HCl, 10.3 mM of N-methyl-2phenylindole (NMFI), and methanesulfonic acid at 45°C. The lipid peroxidation was analyzed based on the standard curve of the malondialdehyde (MDA) concentrations, measured by its absorbance at a wavelength of 570 nm (de Carvalho et al, 2019 ). The MDA concentration was quantified in nmols per milligram of protein determined using the Bradford method.…”

Section: Methodsmentioning

confidence: 99%

Putative Activation of the CB1 Cannabinoid Receptors Prevents Anxiety-Like Behavior, Oxidative Stress, and GABA Decrease in the Brain of Zebrafish Submitted to Acute Restraint Stress

Luz

Santos-Silva

Cardoso

et al. 2021

Front. Behav. Neurosci.

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Anxiety disorder is a well-recognized condition observed in subjects submitted to acute stress. Although the brain mechanisms underlying this disorder remain unclear, the available evidence indicates that oxidative stress and GABAergic dysfunction mediate the generation of stress-induced anxiety. Cannabinoids are known to be efficient modulators of behavior, given that the activation of the cannabinoid receptors type-1 (CB1 receptors) induces anxiolytic-like effects in animal models. In the present study, we aimed to describe the effects of the stimulation of the CB1 receptors on anxiety-like behavior, oxidative stress, and the GABA content of the brains of zebrafish submitted to acute restraint stress (ARS). The animals submitted to the ARS protocol presented evident anxiety-like behavior with increased lipid peroxidation in the brain tissue. The evaluation of the levels of GABA in the zebrafish telencephalon presented decreased levels of GABA in the ARS group in comparison with the control. Treatment with ACEA, a specific CB1 receptor agonist, prevented ARS-induced anxiety-like behavior and oxidative stress in the zebrafish brain. ACEA treatment also prevented a decrease in GABA in the telencephalon of the animals submitted to the ARS protocol. Overall, these preclinical data strongly suggest that the CB1 receptors represent a potential target for the development of the treatment of anxiety disorders elicited by acute stress.

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Oxidative Stress Mediates Anxiety-Like Behavior Induced by High Caffeine Intake in Zebrafish: Protective Effect of Alpha-Tocopherol

Cited by 36 publications

References 36 publications

Effects of several tea extracts on nonalcoholic fatty liver disease in mice fed with a high‐fat diet

Effects of several tea extracts on nonalcoholic fatty liver disease in mice fed with a high‐fat diet

Anxiolytic-like effects of Moringa oleifera in Swiss mice

Putative Activation of the CB1 Cannabinoid Receptors Prevents Anxiety-Like Behavior, Oxidative Stress, and GABA Decrease in the Brain of Zebrafish Submitted to Acute Restraint Stress

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