Oxidative Stress Markers and Their Dynamic Changes in Patients after Acute Ischemic Stroke

Abstract

We have focused on determining the range of oxidative stress biomarkers and their dynamic changes in patients at different time points after the acute ischemic stroke (AIS). 82 patients with AIS were involved in our study and were tested: within 24 h from the onset of the attack (group A); at 7-day follow-up (group B); and at 3-month follow-up (group C). 81 gender and age matched volunteers were used as controls. Stroke patients in group A had significantly higher concentrations of plasma lipid peroxides and urine 8-isoprostanes when compared with controls. Protein carbonyls were not significantly different in any experimental group compared to controls. Antioxidant capacity of plasma was increased only in experimental group C. Activities of superoxide dismutase and catalase were elevated in all three experimental AIS groups compared to controls. Paraoxonase activity was reduced in groups A and B and unchanged in group C when compared to controls. Glutathione peroxide activity was elevated only in group A. Our results suggest that free radical damage is the highest within 24 h after the attack. During the next 3 months oxidative damage to lipids caused by free radicals is reduced due to activated antioxidant system.

Can oxidative stress markers help define stroke prognosis?

“…Paspalj et al [3] point out that the levels of catalase demonstrated a slight increase in patients who did not survive, but no statistical relevance was verified. But for Žitnanova [5], there was a statistically significant increase in catalase levels, especially on the seventh day. In another aspect, Chen et al [12] comprehend thiol as a relevant biomarker for the measurement of increased oxidative damage.…”

“…However, Žitnanova [5] produced a sample with a statistically significant elevation of SOD in larger quantities on the seventh day after stroke onset. It could be construed as an adaptive response of the organism to pre-ischemic conditioning.…”

“…A marked disturbance of the AIS metabolism in serum was found, mainly associated with amino acid related metabolism. Tyrosine, lactate and tryptophan can be considered as potential AIS markers [5] To determine the range of oxidative stress markers and antioxidants in stroke patients over a 3-month period following acute ischemic stroke.…”

“…Unfortunately, reactive oxygen species (ROS) increase after a stroke, causing damage to vascular brain structures and compromising physical-chemical interactions [5]. The production of free radicals is physiological, being important for biochemical reactions, in the production of energy, as well as a defense mechanism, among other functions [6].…”

The Influence of Cat - 262 C/T Polymorphism on Catalase Activity in Patients With Ischemic Stroke

“…It catalyzes the decomposition reaction of H2O2 to water and molecular oxygen (7). Although numerous studies point to the increased production of ROS in AIS, the results of antioxidant enzymes activities in this disease are contradictory (8)(9)(10).…”