Oxidative stress is inhibited by plant-based supplements: A quantitative lipidomic analysis of antioxidant activity and lipid compositional change

Data for “Oxidative stress is inhibited by plant-based supplements: a quantitative lipidomic analysis of antioxidant activity and lipid compositional change”

“…Numerical values of the PC: PE ratio and double bond index (DBI) are also provided. These data support of our recent publication [2] where the inhibitory effects of five plant-based supplements were investigated. This data-in-brief article adds to the existing publication by providing the scientific community with both the raw HPLC- MS/MS data and the processed numerical lipidomics data, used to generate the plots in our publication.…”

“…The antioxidant effect of five different plant extract supplements formed the basis of the experiment, which contrasts lipid oxidation in the presence of these to a control, no supplement, dataset. These data support a recent publication [2] where the possible antioxidant properties of supplements derived from plant extracts were measured using a targeted lipidomics approach. The motivation for the research was to understand how oxidative stress impacts membrane lipid composition [3] , collective membrane physical properties [4] and any ameliorating properties of dietary supplements in this context.…”

“…The following materials and methods were used throughout the study as described in full in our recent publication [2] , a brief summary of the aspects key to using the data presented follows.…”

“…Lipids were separated using reversed phase chromatography (ACQUITY UPLC HSS T3 Column (100Å, 1.8 µm, 2.1 mm X 100 mm, Waters Corporation) with a solvent gradient consisting of solvent A; (60:40 v/v); water: acetonitrile and 10 mM ammonium formate and solvent B; (90:10) isopropanol: acetonitrile and 10 mM ammonium formate at a flow rate of 200 µL/min. Full details of the method have been published previously [2] .…”

“…Filtering was performed using the peak picking ‘Vendor’ algorithm at MS level 1. Lipid identities were assigned using Lipidex software [10] , using the Lipidex_HCD_Formic, Lipidex_HCD_Hydroxy, Lipidex_HCD_Plants, Lipidex_HCD_ULCFA and Lipidex_Splash_ISTD_Formic spectral libraries, using the spectral searching parameters specified previously [2] . HPLC alignment files were generated using MZMine [8] , whereby Thermofisher raw format (.raw) files were imported to MZMine and features were detected using the settings identified in our previous publication [2] .…”