Oxidative stress is H2O2 under the bridge without MED8

Hammoudi, Valentin

doi:10.1093/plcell/koab078

Cited by 4 publications

(5 citation statements)

References 4 publications

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“…Among them, 474 genes were up‐regulated and 482 genes were down‐regulated in med8 mutants (Figure S4; Dataset S1). According to gene ontology (GO) analysis, the genes that were up‐regulated and down‐regulated in med8 mutants were enriched in various biological processes, including systemic acquired resistance, defense response, response to oomycetes, response to fungus, response to bacterium, response to jasmonic acid, cellular response to hypoxia (Figure S4), consistent with previous observations (Hammoudi, 2021; Wang et al., 2011; Xu & Li, 2012; Zhang et al., 2021).…”

Section: Resultssupporting

confidence: 88%

MED8 regulates floral transition in Arabidopsis by interacting with FPA

Chen

Liu

et al. 2023

The Plant Journal

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SUMMARYSuccess in plant reproduction is highly dependent on the correct timing of the floral transition, which is tightly regulated by the flowering pathways. In the model plant Arabidopsis thaliana, the central flowering repressor FLOWERING LOCUS C (FLC) is precisely regulated by multiple flowering time regulators in the vernalization pathway and autonomous pathway, including FPA. Here we report that Arabidopsis MEDIATOR SUBUNIT 8 (MED8) promotes floral transition in Arabidopsis by recruiting FPA to the FLC locus to repress FLC expression. Loss of MED8 function leads to a significant late‐flowering phenotype due to increased FLC expression. We further show that MED8 directly interacts with FPA in the nucleus and recruits FPA to the FLC locus. Moreover, MED8 is indispensable for FPA's function in controlling flowering time and regulating FLC expression. Our study thus reveals a flowering mechanism by which the Mediator subunit MED8 represses FLC expression by facilitating the binding of FPA to the FLC locus to ensure appropriate timing of flowering for reproductive success.

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Section: Resultssupporting

confidence: 88%

MED8 regulates floral transition in Arabidopsis by interacting with FPA

Chen

Liu

et al. 2023

The Plant Journal

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“…Salt stress can induce ROS, which leads to secondary oxidation stress, disturbs cellular redox homeostasis, and damages cell component and structure. H 2 O 2 , as one of the most abundant ROS in cells, is a key signaling molecule for plant growth, development, and resistance to stress [ 22 ]. In this study, we observed that the H 2 O 2 content was significantly elevated under salt treatment, but slightly decreased at 144 h. Notably, MDA content, another stress-related physiological parameter, also demonstrated a significant increasing trend under stress with a slight decline at 144 h. These results suggested that early stages of salt stress in G. anomalum featured severe oxidative and membrane damage, but alleviation of oxidative damage occurs in the late stress stage.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Expression Profiling Reveals the Molecular Response to Salt Stress in Gossypium anomalum Seedlings

Yu,

Guo,

et al. 2024

Plants

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Some wild cotton species are remarkably tolerant to salt stress, and hence represent valuable resources for improving salt tolerance of the domesticated allotetraploid species Gossypium hirsutum L. Here, we first detected salt-induced stress changes in physiological and biochemical indexes of G. anomalum, a wild African diploid cotton species. Under 350 mmol/L NaCl treatment, the photosynthetic parameters declined significantly, whereas hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents increased. Catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activity and proline (PRO) content also significantly increased, reaching peak values at different stages of salt stress. We used RNA-Seq to characterize 15,476 differentially expressed genes in G. anomalum roots after 6, 12, 24, 72, and 144 h of salt stress. Gene Ontology enrichment analysis revealed these genes to be related to sequence-specific DNA and iron ion binding and oxidoreductase, peroxidase, antioxidant, and transferase activity; meanwhile, the top enriched pathways from the Kyoto Encyclopedia of Genes and Genomes database were plant hormone signal transduction, phenylpropanoid biosynthesis, fatty acid degradation, carotenoid biosynthesis, zeatin biosynthesis, starch and sucrose metabolism, and MAPK signaling. A total of 1231 transcription factors were found to be expressed in response to salt stress, representing ERF, MYB, WRKY, NAC, C2H2, bZIP, and HD-ZIP families. Nine candidate genes were validated by quantitative real-time PCR and their expression patterns were found to be consistent with the RNA-Seq data. These data promise to significantly advance our understanding of the molecular response to salt stress in Gossypium spp., with potential value for breeding applications.

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“…Until now plenty of studies have been conducted regarding the implication of ROS in oxidative stress responses and modulation of gene expression (44). However, still less is known about the exact mechanisms with respect to involvement of H 2 O 2 , as one the most abundant forms of ROS in cells, in how the gene expression patterns related to the activation of defense mechanisms were modulated (45). In the current study, we evaluated the effect of H 2 O 2 on the mRNA levels of ED-related genes i.e., EDN1, NOS3, VCAM1, and SERPINE1 (46) in HUVEC cells and we observed signifi cant alterations.…”

Section: Discussionmentioning

confidence: 99%

Can atypical response in endothelial dysfunction-related genes and microRNAs arise from low hydrogen peroxide exposure?

URHAN-KUCUK,

TERZI

2024

BLL

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OBJECTIVE: Vascular endothelium is a tissue in which several vasoactive substances are produced and secreted. Reactive oxygen species can cause endothelial dysfunction (ED). miRNAs can be implicated in the oxidative stress-related ED during vascular disease pathogeneses. Our aim is to investigate effect of H 2 O 2induced oxidative stress on expression levels of genes and miRNAs that are key players in ED. METHODS: H 2 O 2 effect on cell viability of human umbilical-vein endothelial cells (HUVEC) at 24-hour was measured with MTT. Low sub-cytotoxic H 2 O 2 concentrations (25, 50 μM) were selected to analyze their oxidative stress-inducing capacities with MDA assay and their effects on EDN1, NOS3, VCAM1, SERPINE1, miR21, miR22, miR126, and miR146a levels with RT-qPCR. RESULTS: Each tested H 2 O 2 concentration reduced HUVEC cell viability. Fifty μM H 2 O 2 augmented cellular MDA levels. Intriguingly, EDN1, VCAM1, and SERPINE1 and all analyzed miRNAs' levels attenuated upon H 2 O 2 treatment whereas there was no change in NOS3 levels compared to control. There was a positive correlation between miR-21 and VCAM1. CONCLUSION: Rat her than individual alterations in analyzed parameters, consistent changes in our fi ndings i.e., parallel decreases in EDN1, VCAM1, SERPINE1 mRNA levels as well as miRNAs, suggests that H 2 O 2 concentration-dependent modulation of expression patterns can bring about various impacts on ED (Tab. 1, Fig. 5, Ref. 63). Tex t in PDF www.elis.sk KEY WORDS: oxidative stress, endothelial dysfunction, hydrogen peroxide (H 2 O 2 ), microRNA, gene expression.

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Oxidative stress is H2O2 under the bridge without MED8

Cited by 4 publications

References 4 publications

MED8 regulates floral transition in Arabidopsis by interacting with FPA

MED8 regulates floral transition in Arabidopsis by interacting with FPA

Transcriptome Expression Profiling Reveals the Molecular Response to Salt Stress in Gossypium anomalum Seedlings

Can atypical response in endothelial dysfunction-related genes and microRNAs arise from low hydrogen peroxide exposure?

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