Oxidative stress induced in rice suspension cells exposed to microcystin-LR at environmentally relevant concentrations

Chen, Shihui; Jiang, Jinlin; Long, Tao; Zhu, Xingcheng; Zhang, Huanchao; Deng, Shaopo; Liu, Renbin

doi:10.1007/s11356-021-13353-3

Cited by 10 publications

(3 citation statements)

References 66 publications

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“…During bloom outbreaks, the dissolved MC concentrations in water normally range between 0.1 and 10 μg·L −1 , while the ecological risks of low MC concentrations in natural environments remain unclear, and the risks that are associated with MCs in the food chain have not been fully elucidated [ 1 , 2 , 9 ]. Moreover, MCs can enter farmland via irrigation water [ 10 , 11 ] and induce phytotoxicity in terrestrial plants [ 12 , 13 , 14 , 15 , 16 ]. Previous research has already shown that MCs can accumulate in the plant body via spray irrigation [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…It has been demonstrated that low concentrations of MC-LR can promote the increase of rice biomass (including plant height, root length, and fresh weight), while, at higher concentrations, MC-LR plays an inhibitory role on vegetative growth, leaving the plants small, with yellow leaves [ 10 ]. The mechanism of how MCs influence crop growth has not been well elucidated [ 11 ]. Sucrose synthase (SS) is a glycosyl transferase enzyme that plays a key role in sugar metabolism of plants.…”

Section: Introductionmentioning

confidence: 99%

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Bioaccumulation of Microcystin-LR and Induced Physio-Biochemical Changes in Rice (Oryza sativa L.) at Vegetative Stage under Hydroponic Culture Conditions

Jiang,

Shi,

Tian

et al. 2024

Toxins

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Irrigation with water containing a variety of microcystins (MCs) may pose a potential threat to the normal growth of agricultural plants. To investigate the phytotoxicity of MC-LR at environmental concentrations on rice (Oryza sativa L.), the characteristics of uptake and accumulation in plant tissues, as well as a series of key physio-biochemical process changes in leaves of rice seedlings, were measured at concentrations of 0.10, 1.0, 10.0, and 50.0 μg·L−1 in hydroponic nutrient solutions for 7, 15, 20, and 34 days. Results showed that MC-LR could be detected in rice leaves and roots in exposure groups; however, a significant accumulation trend of MC-LR in plants (BCF > 1) was only found in the 0.10 μg·L−1 group. The time-course study revealed a biphasic response of O2•− levels in rice leaves to the exposure of MC-LR, which could be attributed to the combined effects of the antioxidant system and detoxification reaction in rice. Exposure to 1.0–50.0 μg·L−1 MC-LR resulted in significant depletion of GSH and MDA contents in rice leaves at later exposure times (15–34 days). Low MC-LR concentrations promoted nitric oxide synthase (NOS) activity, whereas high concentrations inhibited NOS activity during the later exposure times. The reduced sucrose synthase (SS) activities in rice exposed to MC-LR for 34 days indicated a decrease in the carbon accumulation ability of plants, and therefore may be directly related to the inhibition of plant growth under MC exposure. These findings indicate that the normal physiological status would be disrupted in terrestrial plants, even under exposure to low concentrations of MC-LR.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of Microcystin-LR and Induced Physio-Biochemical Changes in Rice (Oryza sativa L.) at Vegetative Stage under Hydroponic Culture Conditions

Jiang,

Shi,

Tian

et al. 2024

Toxins

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show abstract

“…During bloom outbreaks, dissolved MCs concentrations in water typically range between 0.1 and 10 μg•L −1 , while ecological risks of the low MCs concentrations in natural environments remain unclear, and the risks that associated with MCs in the food chain have not been fully elucidated [1,2,9]. Moreover, MCs can enter farmland via irrigation water and will inevitably harm terrestrial plants [10,11], however, very little known about the mechanism of MC-induced phytotoxicity on terrestrial plants [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of Microcystin-LR and Induced Physio-Biochemical Changes in Rice (<em>Oryza sativa</em> L.) at Vegetative Stage under Hydroponic Culture Conditions

Jiang,

Shi,

Tian

et al. 2023

Preprint

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Irrigation with water containing a variety of microcystins (MCs) may pose potential threat to normal growth of agricultural plants. The mechanism of microcystin-LR (MC-LR) induced phytotoxicity in rice (Oryza sativa L.) at environmental concentrations is still unknown. Rice seedlings were exposed to MC-LR at concentrations of 0.10, 1.0, 10.0 and 50.0 μg·L−1 in hydroponic nutrient solutions for 7, 15, 20, and 34 days in the current study. The absorption and accumulation in leaf and root tissues, as well as a series of key physio-biochemical process changes in leaves of rice at different exposure time points were measured. Results showed that MC-LR could be detected in rice leaves and roots in all exposure groups, however, a significant accumulation trend of MC-LR in plants (BCF > 1) was only found in the lowest group (0.10 μg·L−1). The time-course study revealed a biphasic response of O2•- levels in rice leaves to the exposure of MC-LR, which was more pronounced in higher concentration groups, which could be attributed to the combined effects of antioxidant and detoxification mechanisms in rice. Exposure to 1.0 - 50.0 μg·L−1 MC-LR resulted in significant depletion of GSH and MDA contents in rice leaves at later exposure times. The changes of nitric oxide synthase (NOS) in rice leaves under MC-LR exposure were firstly investigated in the current study. Low MC-LR concentrations promoted NOS activity, whereas high concentrations inhibited NOS activity during the later exposure times, implying that NO may play a role in MC-LR toxicity in rice. Reduced sucrose synthase (SS) activities in rice exposed to MC-LR can reduce the plant's ability to accumulate carbon and thus may be directly related to the reduction in vegetative growth. These findings suggest that even at low concentrations of MC-LR, terrestrial plants' normal physiological status is disrupted, which, when combined with previous findings, helps reveal the mechanism of MC-LR-induced phytotoxicity.

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As(III)-Oxidizing Bacteria Alleviate Arsenite Toxicity via Reducing As Accumulation, Elevating Antioxidative Activities and Modulating Ionome in Rice (Oryza sativa L.)

et al. 2023

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Compared to other crops, paddy rice trends to accumulate more arsenic (As) from soils. The toxicity and mobility of As mainly depended on its chemical species. To mitigate As(III) stress to rice plants, As(III)oxidizing strain SMS11 isolated from As-contaminated soils was employed in the current study. The rice seedlings were hydroponically cultivated in three groups: Control Group (without any addition), As(III) group (amended with 1 mg•L -1 As(III)) and As(III)+B group (treated with As(III) and As(III)-oxidizing bacteria). Compared to Control group, the growth of rice shoots from As(III) group was signi cantly restricted. Inoculation with the bacteria could not only enhance the growth of the rice plants, but also signi cantly reduce the As level in the rice shoots. Evaluation of oxidative stress indices showed that superoxide dismutase activity in the rice plants was enhanced under As(III) stress, resulting in promoted inhibition ability of superoxide anion radical (O 2•− ). The activity of catalase in the rice shoots was weakened when exposed to As(III), increasing the risk of hydroxyl radical ( • OH) formation. When cocultivated with the bacteria, • OH formation was signi cantly inhibited in the rice shoots. Uptake of As elevated most nutrient element contents in the rice shoots simultaneously. The levels of most nutrient elements in the rice shoots were declined with alleviation of As stress after inoculation with the bacteria. The ionomics of the rice plants revealed signi cant variations with tissues and environmental conditions.The results represented ionomic pro le as a promising tool to identify rice plants under different external conditions.

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Oxidative stress induced in rice suspension cells exposed to microcystin-LR at environmentally relevant concentrations

Cited by 10 publications

References 66 publications

Bioaccumulation of Microcystin-LR and Induced Physio-Biochemical Changes in Rice (Oryza sativa L.) at Vegetative Stage under Hydroponic Culture Conditions

Bioaccumulation of Microcystin-LR and Induced Physio-Biochemical Changes in Rice (Oryza sativa L.) at Vegetative Stage under Hydroponic Culture Conditions

Bioaccumulation of Microcystin-LR and Induced Physio-Biochemical Changes in Rice (<em>Oryza sativa</em> L.) at Vegetative Stage under Hydroponic Culture Conditions

As(III)-Oxidizing Bacteria Alleviate Arsenite Toxicity via Reducing As Accumulation, Elevating Antioxidative Activities and Modulating Ionome in Rice (Oryza sativa L.)

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