Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis

Abstract

Introduction andObjective. Human oxidative stress-induced growth inhibitor 1 (OSGIN1) is a protein identified in 2001 which belongs to the OKL38 protein family. The aim of the study was to investigate the levels of this protein depending on the severity of alcohol-induced liver cirrhosis. Materials and method. The study group consisted of 60 patients: 30 patients with cirrhosis in the P-Ch A and B stage and 30 in the P-Ch C stage. The control group consisted of 18 healthy individuals without liver diseases, who did not abuse alcohol. Oxidative stress induced growth inhibitor 1 (OSGIN1), fibroblast growth factor 1 (FGF1) and fibroblast growth factor 21 (FGF21) were determined in blood serum using enzyme-linked immunosorbent assay (ELISA) kits. All absorbance readings were conducted using an Epoch Microplate Spectrophotometer (BioTek Instrumentals, Inc., Winooski, VT, USA). OSGIN1, FGF1 and FGF21 concentrations were determined using Sandwich enzyme immunoassay kits (by Cloud Clone Corp., Katy, TX, USA). Statistica 13.3 (TIBCO Software, Inc.) was used for data analysis. Results. The concentration of OSGIN1 was 0.028 ± 0.017 in the control group which increased with the advancement of liver cirrhosis (stage of Pugh-Child): 0.075 ± 0.098 in the P-Ch A + B group and 0.121 ± 0.134 in the P-Ch C stage. Multiple comparison tests confirmed statistically significant differences in OSGIN1 concentration between the control group and P-Ch C (p <0.02). Significant correlations were noted between OSGIN1 and FGF1 (r = 0.39; p = 0.004) and between OSGIN1 and FGF21 (r = 0.53; p <0.0001). Conclusions. The study revealed that the level of OSGIN1 increased significantly in the P-Ch C stage of liver cirrhosis. It is possible that OSGIN1 may be used for the non-invasive diagnosis of ALD, but its possible diagnostic value is still very uncertain.

The crosstalk between fibroblast growth factor 21 (FGF21) system and substance use

“…They found that OSGIN1 and FGF21 levels were elevated in people with ALC, and that levels of OSGIN1 correlated with levels of FGF21. Interestingly, OSGIN1 levels were highest in the cirrhosis group at the Child-Pugh C stage, while FGF21 levels were highest in the combined Child-Pugh A/B group compared to Child-Pugh C. 71 Another study that measured serum FGF21 levels in people with alcohol-associated hepatitis vs. people with decompensated alcohol-associated cirrhosis found elevated FGF21 levels in the hepatitis group compared to cirrhosis. 72 Together, these data indicate that acute alcohol exposure increases FGF21, and that ALD is associated with elevated FGF21 levels, which may be blunted in more severe forms of ALD in which the liver is cirrhotic and reduced overall functioning.…”

“… 62 Alcohol Humans Individuals with varying degrees of alcohol-related cirrhosis using the Child-Pugh score and related categories (P-Ch A, B, and C) and Healthy Controls FGF21 levels increased in participants with liver cirrhosis compared to HC FGF21 levels were highest in P-Ch A and B grp compared to P-Ch C Sak et al. 71 Alcohol Humans People with varying degrees of ALD: alcohol-associated hepatitis vs. decompensated alcohol-associated cirrhosis groups. Serum FGF21 measured in both groups.…”