Oxidative Stress-Induced Bioprospecting of Microalgae

Chokshi, Kaumeel; Pancha, Imran; Ghosh, Arup; Mishra, Sandhya

doi:10.1007/978-3-319-62094-7_13

Cited by 4 publications

(3 citation statements)

References 127 publications

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“…Lutein is an antioxidant that protects microalgal cells from the toxicity of reactive oxygen species produced under extreme environmental conditions (Chokshi et al 2017). We observed an increase in lutein production under each of the three tested abiotic-stress conditions, especially under HL where the lutein content was significantly higher than that under the other stress conditions.…”

Section: β-Carotene and Lutein Accumulationmentioning

confidence: 65%

Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalga Dunaliella salina Y6

Zhu

et al. 2020

Ann Microbiol

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Purpose: Under different abiotic-stress conditions, the unicellular green microalga Dunaliella salina accumulates large amounts of carotenoids which are accompanied by fatty acid biosynthesis. Carotenoids and fatty acids both possess long carbon backbones; however, the relationship between carotenoid and fatty acid metabolism is controversial and remains poorly understood in microalgae. Methods: In this study, we investigated the growth curves and the β-carotene, lutein, lipid, and fatty acid contents of D. salina Y6 grown under different abiotic-stress conditions, including high light, nitrogen depletion, and high salinity. Results: Both high-salinity and nitrogen-depleted conditions significantly inhibited cell growth. Nitrogen depletion significantly induced β-carotene accumulation, whereas lutein production was promoted by high light. The accumulation of lipids did not directly positive correlate with β-carotene and lutein accumulation under the three tested abiotic-stress conditions, and levels of only a few fatty acids were increased under specific conditions. Conclusion: Our data indicate that cellular β-carotene accumulation in D. salina Y6 positive correlates with accumulation of specific fatty acids (C16:0, C18:3n3, C14:0, and C15:0) rather than with total fatty acid content under different abiotic stress conditions.

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Section: β-Carotene and Lutein Accumulationmentioning

confidence: 65%

Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalga Dunaliella salina Y6

Zhu

et al. 2020

Ann Microbiol

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“…Induction of oxidative stress using ROS reagent in Chlorella zofingen sis, was also effective to increase the carotenoid accumu lation (Hu et al 2018). It is known that the addition of various ROS reagents into the culture medium was able to improve the carotenoid synthesis in microalgae (Chokshi et al 2017).…”

Section: Introductionmentioning

confidence: 97%

Enhanced astaxanthin production by oxidative stress using methyl viologen as a reactive oxygen species (ROS) reagent in green microalgae Coelastrum sp.

Tharek

Suzuki

Hara

et al. 2020

Indones. J. Biotechnol.

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Microalgae are known to be a potential resource of high-value metabolites that can be used in the growing field of biotechnology. These metabolites constitute valuable compounds with a wide range of applications that strongly enhance a bio-based economy. Among these metabolites, astaxanthin is considered the most important secondary metabolite, having superior antioxidant properties. For commercial feasibility, microalgae with enhanced astaxanthin production need to be developed. In this study, the tropical green microalgae strain, Coelastrum sp., isolated from the environment in Malaysia, was incubated with methyl viologen, a reactive oxygen species (ROS) reagent that generates superoxide anion radicals (O2-) as an enhancer to improve the accumulation of astaxanthin. The effect of different concentrations of methyl viologen on astaxanthin accumulation was investigated. The results suggested that the supplementation of methyl viologen at low concentration (0.001 mM) was successfully used as a ROS reagent in facilitating and thereby increasing the production of astaxanthin in Coelastrum sp. at a rate 1.3 times higher than in the control.

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“…The stability of the cell and the ability to maintain its homeostasis both under physiologically normal conditions and under the influence of stress factors is provided by AOS [ 12 ]. Any negative impact and realisation of physiological functions of the body are accompanied by the generation of free radicals, which are intermediaries in the transmission of intracellular signals, the formation of a protective reaction of the body, and in the case of excessive production, the initiation of lipid peroxidation and oxidative modification of biopolymers and metabolites, and the development of oxidative stress, which leads to cell death [ 15 , 16 , 17 ]. To prevent the result of oxidative modification of cell components, reactive oxygen species (ROS) are neutralised by AOS components, which include enzymes and low-molecular-weight antioxidants and also implement genetically programmed mechanisms to increase oxidative resistance by regulating the metabolism of individual cell components, one of which is a change in the ratio of saturated fatty acids (SFAs) and unsaturated (UFAs) in the composition lipids, as well as a decrease in the intensity of energy metabolism [ 18 , 19 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Status and Biotechnological Potential of New Vischeria vischeri (Eustigmatophyceae) Soil Strains in Enrichment Cultures

Yakoviichuk¹,

Krivova

Maltseva

et al. 2023

Antioxidants

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The functional state of enrichment cultures of the Eustigmatophycean strains Vischeria vischeri MZ–E3 and MZ–E4 after 25-day cultivation in the BBM medium was studied. The concentrations of chlorophyll a, total carotenoids, protein, vitamins A and E, fatty acid peroxidation product content, an antioxidant enzyme, and succinate dehydrogenase activity were measured. MZ–E3 succinate dehydrogenase activity was significantly higher by 2.21 times; the MZ–E4 strain had 2.94 times higher glutathione peroxidase activity. The MZ–E3 antioxidant activity index and the MZ–E3 unsaturation of fatty acids were 1.3 and 1.25 times higher than the MZ–E4. The retinol and α-tocopherol content of the MZ–E3 was 28.6% and 38.76% higher than MZ–E4. The main fatty acid profile differences were the 3.46-fold and 3.92-fold higher stearic and eicosapentaenoic acid content in the MZ–E4 biomass. MZ–E3 had higher antioxidant, energy, and metabolic and photosynthetic status than MZ–E4. The antioxidant status of the studied strains showed the dependence of the adaptive mechanisms of each, associated with differences in the ecological conditions of the biotopes from which they were isolated. These strains are promising for producing α-tocopherol and biomass enriched with omega-3 and omega-6 fatty acids.

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Oxidative Stress-Induced Bioprospecting of Microalgae

Cited by 4 publications

References 127 publications

Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalga Dunaliella salina Y6

Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalga Dunaliella salina Y6

Enhanced astaxanthin production by oxidative stress using methyl viologen as a reactive oxygen species (ROS) reagent in green microalgae Coelastrum sp.

Antioxidant Status and Biotechnological Potential of New Vischeria vischeri (Eustigmatophyceae) Soil Strains in Enrichment Cultures

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