Oxidative stress index as a biochemical parameter in major depressive disorder

Abstract

Background: Oxidative stress is considered to be involved in pathogenesis of different diseases including Major Depressive Disorder (MDD). In laboratory, this can be measured by a biochemical parameter called Oxidative stress Index (OSI), which can be calculated as the ratio percentage of Total peroxide to the total anti-oxidant potential.Aims and Objectives: This study was undertaken to determine OSI in MDD and healthy control and to find out whether any significant difference exists among the mean values of OSI in MDD and healthy controls. It was also aimed to find out whether OSI can be correlated with the severity of MDD. The validity of OSI as a biochemical parameter to diagnose MDD was also evaluated.Materials and Methods: In this study OSI was determined in 101 cases of Major Depressive Disorder (MDD) along with 106 age and sex matched controls by measuring Total Peroxides (TP) and Total Antioxidant Capacity (TAC). TP was measured by (FOX 2) method and TAC was measured by The Ferric Reducing Ability of Plasma (FRAP) method. The correlation of OSI & severity of depression was assessed by Spearman rank Correlation test. ROC curve was used for determining the validity of OSI for diagnosis of Depression.Result: Significant increase in OSI was observed in MDD (2.33 + 0.457) when compared to healthy controls (1.311 + 0.352). The increase was also found to be associated with severity of MDD (Spearman coefficient of rank correlation, rho = 0.289). The diagnostic ability of OSI was evaluated by ROC curve, which showed Area under curve as 0.96. The optimal cut off value of OSI was found to be 1.83, with 87.13% sensitivity and 92.45% specificity.Conclusion: Oxidative stress may play a role in pathogenesis of MDD as indicated by measuring OSI. This parameter is found to be significantly associated with severity of disease. The diagnostic ability of OSI for MDD is quite satisfactory. However, further study is needed to validate this finding.Asian Journal of Medical Sciences Vol.7(5) 2016 31-35

Depression and anxiety symptoms are associated with prooxidant-antioxidant balance: A population-based study

“…In another study, OSI was determined in 101 cases with MDD along with 106 age and sex matched controls. Similarly, a significant increase in OSI was reported in patients with MDD when compared to healthy controls, which was associated with the severity of the disease (Gupta et al, 2016). Yanik et al observed a significantly higher OSI values in MDD patients than those of controls as well as a significant positive correlation between OSI values and Hamilton Depression Rating Scale (HDRS) (Yanik et al, 2004).…”

“…Furthermore, a limited number of studies have also reported a significant positive association between severity of depression and serum oxidative stress index (OSI), defined as the ratio of total oxidant status (TOS) to total antioxidant capacity (TAC) (Cumurcu et al, 2009;Gupta et al, 2016;Yanik et al, 2004). Cumurcu et al conducted a study on 55 MDD patients and 40 healthy controls and found significantly higher OSI in the MDD group compared with those of the controls.…”

Impaired PRR expression modulates inflammation-triggered oxidative stress and pathogenesis of recurrent vulvovaginal infections

“…A separate evaluation of our results for TOS and TAC, showed that apparently these cases did not experience oxidative stress (OS), as the level of both TAC and TOS was low in cases than controls. Therefore, it is important to evaluate not only the concentrations of oxidants and anti-oxidants separately, but also their relationship through a proportion or ratio, because it is the imbalance between oxidants and anti-oxidants that defines the concept of OS (Gupta et al, 2016). The first approach using this ratio was made in human medicine by Sharma et al (Sharma et al, 1999).…”

Effects of hydrogen sulphide on oxidative stress, inflammatory cytokines, and vascular remodelling in l‐NAME‐induced hypertension

“…OSI was calculated using the following equation 37 CTTCCT-3′ 39 and VCAM-1 forward 5′-GCGAAGGAAACTGGAGA AGACA-3′, reverse 5′-ACACATTAGGGACCGTGCAGTT-3′. 40 qPCR was performed under the following conditions: hot start step at 95°C for 7 minutes, 40 cycles of denaturation for 20 seconds at 95°C, and annealing and extension for 1 minute at 59°C.…”