Oxidative stress in two populations of killifish (Fundulus heteroclitus) with differing contaminant exposure histories

Bacanskas, Lisa R.; Whitaker, Julia; Giulio, Richard T. Di

doi:10.1016/j.marenvres.2004.03.048

Cited by 56 publications

(41 citation statements)

References 8 publications

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“…Of the killifish populations known to have adapted to environmental contaminants, several have adapted specifically to chemicals that act via AhR-dependent mechanisms, and alterations to the AhR pathway seem to be a critical component of the adaptation (Prince and Cooper, 1995a,b;Nacci et al, 1999;Powell et al, 2000;Bello et al, 2001;Ownby et al, 2002;Bacanskas et al, 2004;Hahn et al, 2004). Specifically, all of these populations exhibit a marked reduction in CYP1A inducibility in response to a variety of AhR agonists (Prince and Cooper, 1995a;Elskus et al, 1999;Nacci et al, 1999;Bello et al, 2001;Arzuaga and Elskus, 2002;Meyer et al, 2002.…”

Section: Introductionmentioning

confidence: 99%

Development of the morpholino gene knockdown technique in Fundulus heteroclitus: A tool for studying molecular mechanisms in an established environmental model

et al. 2008

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a b s t r a c t A significant challenge in environmental toxicology is that many genetic and genomic tools available in laboratory models are not developed for commonly used environmental models. The Atlantic killifish (Fundulus heteroclitus) is one of the most studied teleost environmental models, yet few genetic or genomic tools have been developed for use in this species. The advancement of genetic and evolutionary toxicology will require that many of the tools developed in laboratory models be transferred into species more applicable to environmental toxicology. Antisense morpholino oligonucleotide (MO) gene knockdown technology has been widely utilized to study development in zebrafish and has been proven to be a powerful tool in toxicological investigations through direct manipulation of molecular pathways. To expand the utility of killifish as an environmental model, MO gene knockdown technology was adapted for use in Fundulus. Morpholino microinjection methods were altered to overcome the significant differences between these two species. Morpholino efficacy and functional duration were evaluated with molecular and phenotypic methods. A cytochrome P450-1A (CYP1A) MO was used to confirm effectiveness of the methodology. For CYP1A MO-injected embryos, a 70% reduction in CYP1A activity, a 86% reduction in total CYP1A protein, a significant increase in ␤-naphthoflavone-induced teratogenicity, and estimates of functional duration (50% reduction in activity 10 dpf, and 86% reduction in total protein 12 dpf) conclusively demonstrated that MO technologies can be used effectively in killifish and will likely be just as informative as they have been in zebrafish.

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Section: Introductionmentioning

confidence: 99%

Development of the morpholino gene knockdown technique in Fundulus heteroclitus: A tool for studying molecular mechanisms in an established environmental model

et al. 2008

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“…The findings also indicated that induction of GSH synthesis from L-Cys was not a major factor in the increase of hepatic GSH in contaminant-exposed fish whereas it was not accompanied by changes in either L-Cys concentrations or gammaglutamylcysteine synthetase activity (Nishimoto et al, 1995). A population of killifish (F. heteroclitus) inhabiting a creosote-polluted inlet of the Elizabeth River demonstrated higher total glutathione concentrations in adult hepatic tissue as compared to the reference population (Bacanskas et al, 2004). Study on the Indian freshwater fish Wallago attu (Bl.…”

Section: Non-enzymatic Antioxidants: Glutathione and Other Scavengersmentioning

confidence: 88%

“…Dorval et al (2005) demonstrated higher level of hepatic LPO products in white sucker (Catostomus commersoni) from the river sites in Québec (Canada), impacted by agricultural chemicals. The killifish (Fundulus heteroclitus) inhabiting a creosote-polluted inlet of the Elizabeth River also exhibited higher LPO as compared to the reference population (Bacanskas et al, 2004). Differences of the level of TBARS in a liver of common carp (Cyprinus carpio) were also detected between fish from rural and industrial sites in relatively low polluted area in Western Ukraine (Falfushynska & Stoliar, 2009).…”

Section: Oxidative Manifestations: Reactive Oxygen Species Damage Tomentioning

confidence: 92%

“…That is examplified by very few cases stydied to date (Bacanskas et al, 2004, Pandey et al, 2008Falfushynska & Stolyar, 2009;Falfushynska et al, 2010a). The decreased SOD activity along with increased O 2 •-levels suggests the weakness of antioxidant defences in common carp C. carpio at the chronically polluted industrial site (Falfushynska & Stolyar, 2009).…”

Section: Antioxidant Enzymesmentioning

confidence: 98%

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Environmental Pollution and Oxidative Stress in Fish

Stoliar¹,

Lushchak²

2012

Oxidative Stress - Environmental Induction and Dietary Antioxidants

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“…In fish, oxidative stress is mostly studied in environmental contamination by pollutants, particularly heavy metals, pesticides and polycyclic aromatic hydrocarbons (Van der Oost et al 2003;Bacanskas et al 2004;Lushchak 2011). A possible role(s) of the ROS in the pathogenesis of parasitic infections has been an active area of research in recent years.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress in the gill tissues of goldfishes (Carassius auratus) parasitized by Dactylogyrus spp

Mozhdeganloo

Heidarpour

2013

J Parasit Dis

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The aim of this study was to investigate the status of oxidative stress in the gill tissues of goldfishes (Carassius auratus) parasitized by Dactylogyrus spp. We therefore compared the concentrations of malondialdehyde (MDA) and total thiol groups in the gill tissues of parasitized and non-parasitized goldfishes. 15 goldfishes parasitized by Dactylogyrus spp. along with 15 nonparasitized goldfishes were selected for the study. A significant increase in MDA concentration (P \ 0.01) and a significant decrease in total thiol groups in the parasitized group, were observed when compared to the non-parasitized group. This is the first study which evaluates the effect of Dactylogyrus spp. on the oxidative stress status of goldfish. The results of the present study revealed that parasitized goldfishes showed more severe oxidative stress and lipid peroxidation than non-parasitized fishes and enhanced lipid peroxidation may be linked to gill damage in goldfishes parasitized by Dactylogyrus spp.

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Oxidative stress in two populations of killifish (Fundulus heteroclitus) with differing contaminant exposure histories

Cited by 56 publications

References 8 publications

Development of the morpholino gene knockdown technique in Fundulus heteroclitus: A tool for studying molecular mechanisms in an established environmental model

Development of the morpholino gene knockdown technique in Fundulus heteroclitus: A tool for studying molecular mechanisms in an established environmental model

Environmental Pollution and Oxidative Stress in Fish

Oxidative stress in the gill tissues of goldfishes (Carassius auratus) parasitized by Dactylogyrus spp

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