Oxidative stress in the denervated muscle

Abruzzo, Provvidenza Maria; Tullio, Simona di; Marchionni, Cosetta; Belia, Silvia; Fanò, Giorgio; Zampieri, Sandra; Carraro, Ugo; Kern, Helmut; Sgarbi, Gianluca; Lenaz, Giorgio; Marini, Marina

doi:10.3109/10715761003692487

Cited by 47 publications

(45 citation statements)

References 49 publications

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“…As such, our results suggest that the mechanisms driving muscle atrophy with normal aging are unlikely to be the result of accumulated mitochondrial DNA damage. On the other hand, our results in normally aging fasttwitch muscle are very similar to what is seen following surgical denervation where oxidative phosphorylation system subunits are also seen to be elevated in the fast-twitch tibialis anterior muscle (Abruzzo et al, 2010), suggesting denervation could be playing an important role in the aging mitochondrial phenotype.…”

Section: Mitochondrial Dysfunction and Sarcopeniasupporting

confidence: 69%

Alterations in intrinsic mitochondrial function with aging are fiber type‐specific and do not explain differential atrophy between muscles

et al. 2011

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SummaryTo determine whether mitochondrial dysfunction is causally related to muscle atrophy with aging, we examined respiratory capacity, H 2 O 2 emission, and function of the mitochondrial permeability transition pore (mPTP) in permeabilized myofibers prepared from four rat muscles that span a range of fiber type and degree of age-related atrophy. Muscle atrophy with aging was greatest in fast-twitch gastrocnemius (Gas) muscle ()38%), intermediate in both the fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus (Sol) muscles ()21%), and non-existent in adductor longus (AL) muscle (+47%). In contrast, indices of mitochondrial dysfunction did not correspond to this differential degree of atrophy. Specifically, despite higher protein expression for oxidative phosphorylation (oxphos) system in fast Gas and EDL, state III respiratory capacity per myofiber wet weight was unchanged with aging, whereas the slow Sol showed proportional decreases in oxphos protein, citrate synthase activity, and state III respiration. Free radical leak (H 2 O 2 emission per O 2 flux) under state III respiration was higher with aging in the fast Gas, whereas state II free radical leak was higher in the slow AL. Only the fast muscles had impaired mPTP function with aging, with lower mitochondrial calcium retention capacity in EDL and shorter time to mPTP opening in Gas and EDL. Collectively, our results underscore that the age-related changes in muscle mitochondrial function depend largely upon fiber type and are unrelated to the severity of muscle atrophy, suggesting that intrinsic changes in mitochondrial function are unlikely to be causally involved in aging muscle atrophy.

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Section: Mitochondrial Dysfunction and Sarcopeniasupporting

confidence: 69%

Alterations in intrinsic mitochondrial function with aging are fiber type‐specific and do not explain differential atrophy between muscles

et al. 2011

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“…SOD activity was determined in tibialis anterior and soleus skeletal muscles by a competitive colorimetric inhibition assay as previously described [38,39]. This method is based on the ability of superoxide ions, generated by xanthine and xanthine oxidase, (Sigma-Aldrich) to reduce WST-1 (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt (Dojindo Laboratories Co., Kumamoto, Japan) to a water-soluble formazan dye.…”

Section: Sod Activitymentioning

confidence: 99%

Proteomic analysis and protein carbonylation profile in trained and untrained rat muscles

Magherini

Abruzzo

Puglia

et al. 2012

Journal of Proteomics

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Understanding the relationship between physical exercise, reactive oxygen species and skeletal muscle modification is important in order to better identify the benefits or the damages that appropriate or inappropriate exercise can induce. Unbalanced ROS levels can lead to oxidation of cellular macromolecules and a major class of protein oxidative modification is carbonylation. The aim of this investigation was to study muscle protein expression and carbonylation patterns in trained and untrained animal models. We analyzed two muscles characterized by different metabolisms: tibialis anterior and soleus. Whilst tibialis anterior is mostly composed of fast-twitch fibers, the soleus muscle is mostly composed of slow-twitch fibers. By a proteomic approach we identified 15 protein spots whose expression is influenced by training. Among them in tibialis anterior we observed a down-regulation of several glycolitic enzymes. Concerning carbonylation, we observed the existence of a high basal level of protein carbonylation. Although this level shows some variation among individual animals, several proteins (mostly involved in energy metabolism, muscle contraction, and stress response) appear carbonylated in all animals and in both types of skeletal muscle. Moreover we identified 13 spots whose carbonylation increases after training.

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“…[34][35][36][37][38][39][40][41][42] In either case, the progression of denervation in muscle fibers begins with spontaneous activation, i.e. fibrillation, and leads to myofiber atrophy, ultrastructural changes of excitationcontraction coupling, and the gradual loss of excitability in response to external electrical stimulation using standard commercial stimulators.…”

Section: Effects Of Long-lasting Complete Denervation Of Human Musclesmentioning

confidence: 99%

“…fibrillation, and leads to myofiber atrophy, ultrastructural changes of excitationcontraction coupling, and the gradual loss of excitability in response to external electrical stimulation using standard commercial stimulators. 36 Finally, muscles enter severe atrophy, wherein myofibers undergo the internalization of subsarcolemmal myonuclei, resulting in the regrouping of tens of myonuclei within the center of myofibers and the complete disappearance of long segments of the contractile apparatus. 13 Eventually, muscle fibers all but completely disappear, while fibrous and adipose tissue accumulates.…”

Section: Effects Of Long-lasting Complete Denervation Of Human Musclesmentioning

confidence: 99%

3D false color computed tomography for diagnosis and follow-up of permanent denervated human muscles submitted to home-based Functional Electrical Stimulation

2015

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This report outlines the use of a customized false-color 3D computed tomography (CT) protocol for the imaging of the rectus femoris of spinal cord injury (SCI) patients suffering from complete and permanent denervation, as characterized by complete Conus and Cauda Equina syndrome. This muscle imaging method elicits the progression of the syndrome from initial atrophy to eventual degeneration, as well as the extent to which patients' quadriceps could be recovered during four years of home-based functional electrical stimulation (h-b FES). Patients were pre-selected from several European hospitals and functionally tested by, and enrolled in the EU Commission Shared Cost Project RISE (Contract n. QLG5-CT-2001-02191) at the Department of Physical Medicine, Wilhelminenspital, Vienna, Austria. Denervated muscles were electrically stimulated using a custom-designed stimulator, large surface electrodes, and customized progressive stimulation settings. Spiral CT images and specialized computational tools were used to isolate the rectus femoris muscle and produce 3D and 2D reconstructions of the denervated muscles. The cross sections of the muscles were determined by 2D Color CT, while muscle volumes were reconstructed by 3D Color CT. Shape, volume, and density changes were measured over the entirety of each rectus femoris muscle. Changes in tissue composition within the muscle were visualized by associating different colors to specified Hounsfield unit (HU) values for fat, (yellow: [-200; -10]), loose connective tissue or atrophic muscle, (cyan: [-9; 40]), and normal muscle, fascia and tendons included, (red: [41; 200]). The results from this analysis are presented as the average HU values within the rectus femoris muscle reconstruction, as well as the percentage of these tissues with respect to the total muscle volume. Results from this study demonstrate that h-b FES induces a compliance-dependent recovery of muscle volume and size of muscle fibers, as evidenced by the gain and loss in muscle mass. These results highlight the particular utility of this modality in the quantitative longitudinal assessment of the responses of skeletal muscle to long-term denervation and h-b FES recovery.

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Oxidative stress in the denervated muscle

Cited by 47 publications

References 49 publications

Alterations in intrinsic mitochondrial function with aging are fiber type‐specific and do not explain differential atrophy between muscles

Alterations in intrinsic mitochondrial function with aging are fiber type‐specific and do not explain differential atrophy between muscles

Proteomic analysis and protein carbonylation profile in trained and untrained rat muscles

3D false color computed tomography for diagnosis and follow-up of permanent denervated human muscles submitted to home-based Functional Electrical Stimulation

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