Oxidative stress in testis of animals during aging with and without reproductive activity

Salomon, Tiago Boeira; Hackenhaar, Fernanda Schäfer; Almeida, Ana Carolina da Veiga Rodarte de; Schüller, Arthur Krumberg; Alabarse, Paulo Vinícius Gil; Ehrenbrink, Guilherme; Benfato, Mara da Silveira

doi:10.1016/j.exger.2013.06.010

Cited by 27 publications

(23 citation statements)

References 61 publications

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“…Indeed, H 2 O 2 ‐induced oxidative stress in rat Leydig cells has been shown to result in cell apoptosis and testicular dysfunction (Anand et al ., ). Testicular oxidative stress is also involved in ageing and thus causes male infertility (Turner & Lysiak, ; Vazquez‐Memije et al ., ; Salomon et al ., ). Indeed, TBARS and DC levels in testes of aged rats are found significantly increased as compared to those of young ones in this study.…”

Section: Discussionmentioning

confidence: 97%

Carnosine and vitamin E - a promising pair in the combat against testicular oxidative stress in aged rats

et al. 2014

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Oxidative stress is considered to play a key role in ageing. Carnosine alone or together with vitamin E may prove to be helpful in dealing with problems of ageing through its antioxidant activity. Testis, by producing steroids and possessing a poor antioxidant system may become a strong target for the chronic oxidative stress generated during ageing. Therefore we investigated the in vivo effect of carnosine alone or together with vitamin E on testicular oxidative stress in aged rats. In this study, young (5 months) and aged (22 months) Wistar rats were used. Carnosine (250 mg kg(-1); i.p.; 5 days per week) and vitamin E (200 mg kg(-1); i.m.; twice per week) were given to aged rats for 2 months. Increased testicular lipid peroxidation and superoxide dismutase activity in aged rats were declined to the levels of young ones by both treatments. Decreased glutathione peroxidase and glutathione transferase activities returned to the level of young's only by carnosine plus vitamin E treatment. Histopathological evaluation described by Johnsen's score, also showed significant improvement with preserved spermatogenesis. Carnosine plus vitamin E treatment appears to stage a powerful performance by attenuating testicular oxidative stress and sparing the antioxidant system.

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Section: Discussionmentioning

confidence: 97%

Carnosine and vitamin E - a promising pair in the combat against testicular oxidative stress in aged rats

et al. 2014

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“…One of the hypotheses for the effect of the loss of PRDX6 is that there is an ongoing oxidative stress during aging. Indeed, increased levels of reactive oxygen species (ROS) and a decrease in antioxidant enzymes have been reported in aging male rats [40–42] . This aging-associated oxidative stress promotes sperm DNA oxidation and abnormal sperm chromatin in these animals [17,43] .…”

Section: Discussionmentioning

confidence: 99%

Advancing age increases sperm chromatin damage and impairs fertility in peroxiredoxin 6 null mice

et al. 2015

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Due to socioeconomic factors, more couples are choosing to delay conception than ever. Increasing average maternal and paternal age in developed countries over the past 40 years has raised the question of how aging affects reproductive success of males and females. Since oxidative stress in the male reproductive tract increases with age, we investigated the impact of advanced paternal age on the integrity of sperm nucleus and reproductive success of males by using a Prdx6−/− mouse model. We compared sperm motility, cytoplasmic droplet retention sperm chromatin quality and reproductive outcomes of young (2-month-old), adult (8-month-old), and old (20-month-old) Prdx6−/− males with their age-matched wild type (WT) controls. Absence of PRDX6 caused age-dependent impairment of sperm motility and sperm maturation and increased sperm DNA fragmentation and oxidation as well as decreased sperm DNA compaction and protamination. Litter size, total number of litters and total number of pups per male were significantly lower in Prdx6−/− males compared to WT controls. These abnormal reproductive outcomes were severely affected by age in Prdx6−/− males. In conclusion, the advanced paternal age affects sperm chromatin integrity and fertility more severely in the absence of PRDX6, suggesting a protective role of PRDX6 in age-associated decline in the sperm quality and fertility in mice.

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“…Although not significant when compared to the younger control, CUR administration increased plasma testosterone levels (Abarikwu, Akiri, Durojaiye, & Alabi, ). Likewise, PTBW was significantly decreased with age (Salomon et al, ). MLT application in the aged rats had the lowest PTBW when compared with the aged control group.…”

Section: Discussionmentioning

confidence: 98%

“…The weight change among the groups was not significant, but the ratio was found to be significant. For this reason, the ratio has been taken into consideration (Salomon et al, ).…”

Section: Methodsmentioning

confidence: 99%

The protective role of melatonin and curcumin in the testis of young and aged rats

et al. 2018

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We aimed to investigate the effect of melatonin and curcumin treatment on oxidative stress, apoptosis, and histology of testicular tissue in our study. Four groups were formed using young (4 months old, n = 6) and aged (20–22 months old, n = 18) male Wistar albino rats: (a) Young control (1% ethanol:phosphate‐buffered saline [PBS], subcutaneously [s.c.]); (b) Aged control (CTL; n = 6, 1% ethanol:PBS, s.c.); (c) Aged Melatonin (MLT; n = 6, 10 mg/kg, s.c.); (d) Aged Curcumin (CUR; n = 6, 30 mg/kg, i.p.). At the end of 21 days, the rats were sacrificed, and testicular tissues were removed. Malondialdehyde (MDA) in the testicular tissue was determined with thiobarbituric acid reactive substances formation, and glutathione (GSH) was determined with modified Ellman method; testosterone level was determined with chemiluminescence method and histologic changes were determined with Haematoxylin‐Eosin and Johnsen's scoring; Apoptotic cell counts were made with TUNEL staining of seminiferous tubule in testis. With ageing, MDA level increased in testicular tissue, but GSH and blood testosterone levels decreased. Melatonin treatment for aged rats significantly decreased Paired total testicular/body weight ratio compared to aged control group (p < 0.05). Curcumin treatment for aged rats significantly increased GSH level compared to the aged control group (p < 0.05). Besides, melatonin and curcumin treatment significantly decreased the number of apoptotic cells and significantly increased Johnsen's score (p < 0.05).

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Oxidative stress in testis of animals during aging with and without reproductive activity

Cited by 27 publications

References 61 publications

Carnosine and vitamin E - a promising pair in the combat against testicular oxidative stress in aged rats

Carnosine and vitamin E - a promising pair in the combat against testicular oxidative stress in aged rats

Advancing age increases sperm chromatin damage and impairs fertility in peroxiredoxin 6 null mice

The protective role of melatonin and curcumin in the testis of young and aged rats

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