Oxidative stress in skeletal muscle causes severe disturbance of exercise activity without muscle atrophy

Kuwahara, Hirotomo; Horie, Tetsuro; Ishikawa, Shin; Tsuda, Chizuru; Kawakami, Satoru; Noda, Yoichi; Kaneko, Takao; Tahara, Shoichi; Tachibana, Toshiaki; Okabe, Masataka; Melki, Judith; Takano, Ryosuke; Toda, Toshifusa; Morikawa, Daichi; Nojiri, Hidetoshi; Kurosawa, Hisashi; Shirasawa, Takuji; Shimizu, Takahiko

doi:10.1016/j.freeradbiomed.2010.02.011

Cited by 110 publications

(124 citation statements)

References 43 publications

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“…28,[32][33][34] Under normal conditions, redox equilibrium between the production of ROS and the ability of cells to defend against them is maintained by self-regulation. In the perioperative period, ROS is increased by anesthesia, surgical trauma, inflammation and other factors.…”

Section: Discussionmentioning

confidence: 99%

Anti-fatigue Effect of Ginsenoside Rb1 on Postoperative Fatigue Syndrome Induced by Major Small Intestinal Resection in Rat

Tan

Zhou

et al. 2013

Biological & Pharmaceutical Bulletin

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Ginsenoside Rb1 (GRb1), one of the principle active ingredients of Panax ginseng, exerts multiple pharmacological activities to fight fatigue. In the present study, we investigate the anti-fatigue effect of GRb1 on postoperative fatigue syndrome (POFS) in a rat model induced by major small intestinal resection. GRb1 (10 mg/kg) was administrated intraperitoneally once daily for 1, 3, 7, and 10 d from the operation day. Antifatigue effect was assessed by grasping test and biochemical parameters in blood or skeletal muscle were determined by autoanalyzer or commercially available kits. Transmission electron microscope was applied to observe the ultra microstructure of skeletal muscles. The results revealed that GRb1 significantly enhanced rat maximum grip strength with POFS. Similarly, negative alterations in biochemical parameters (lactic acid, hepatic glycogen, muscle glycogen and malondialdehyde) of POFS rats were improved by GRb1. In addition, GRb1 also increased the activity of lactate dehydrogenase and superoxide dismutase in POFS. No significant differences of levels of blood urea nitrogen and ultra microstructure of skeletal muscles were found between the POFS and GRb1 treatment rats. The potent anti-fatigue effect of GRb1 on POFS might be achieved through improvement of energy metabolism and suppression of skeletal muscle oxidative stress.Key words ginsenoside Rb1; postoperative fatigue syndrome; energy metabolism; oxidative stress; animal experiment Ginseng, the dried root of Panax ginseng C. A. MEYER (Araliaceae), has been used as a tonic to treat various disorders in Chinese traditional medicine. It is considered the king of herbs since the earliest Chinese pharmaceutical monograph "Shen Nong Ben Cao Jing." 1) Currently, ginseng is used worldwide as a popular herbal medicine, especially in China, Korea and Japan. Ginsenosides are thought to be the main active ingredients of ginseng with multiple pharmacological activities including anti-aging, anti-carcinogenic, anti-oxidation, antiinflammation, and anti-fatigue.2-6) Modern science has identified more than 50 kinds of ginsenosides. Ginsenoside Rb1 (GRb1), one of the ginsenosides, belongs to the protopanaxadiol group of steroidal saponins. Accumulating evidence indicates that GRb1 exerts a protective effect against stress in various conditions. [7][8][9] Currently, one of the major tasks of surgery is to enhance the recovery after operation. Postoperative fatigue syndrome (POFS) is a common complication after surgery, especially major abdominal and cardiac procedures. 10) For affected patients, POFS manifests itself as a feeling of malaise, lethargy, loss of energy, concentration difficulties and debilitating fatigue. It can persist for up to one month after abdominal operations and gradually resolves in as long as 3 months after uncomplicated gastrointestinal surgery.11-13) Patients who suffer from POFS have a prolonged recovery to normal daily life. In addition, POFS increases health-service costs, burdens patients themselves, their families, hospitals...

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Section: Discussionmentioning

confidence: 99%

Anti-fatigue Effect of Ginsenoside Rb1 on Postoperative Fatigue Syndrome Induced by Major Small Intestinal Resection in Rat

Tan

Zhou

et al. 2013

Biological & Pharmaceutical Bulletin

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“…In the MnSOD -/ -knockout mice (70), the putative SOD mimetic MnTBAP increased the lifespan of the animals only to unmask an underlying neurological deficit. Similarly, the small molecule antioxidants used by Case et al (11) provided partial protection in T-cells from the susceptibility to infection, while Kuwahara et al (58) and Nojiri et al (78) restored exercise capacity in damaged skeletal muscles. Antioxidant supplementation in models of MnSOD deficiency is complex and fraught with unanswered questions involving the selection of an antioxidant(s), the specificity of antioxidants with ROS and RNS, proper dosing regimens, and the bioavailability in the mitochondrial compartment, to name a few.…”

Section: Factors To Consider When Studying Mnsod Tissue-specific Knocmentioning

confidence: 94%

The Use of the Cre/loxPSystem to Study Oxidative Stress in Tissue-Specific Manganese Superoxide Dismutase Knockout Models

Marecki

Parajuli

Crow

et al. 2014

Antioxidants & Redox Signaling

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Significance: Respiring mitochondria are a significant site for reactions involving reactive oxygen and nitrogen species that contribute to irreversible cellular, structural, and functional damage leading to multiple pathological conditions. Manganese superoxide dismutase (MnSOD) is a critical component of the antioxidant system tasked with protecting the oxidant-sensitive mitochondrial compartment from oxidative stress. Since global knockout of MnSOD results in significant cardiac and neuronal damage leading to early postnatal lethality, this approach has limited use for studying the mechanisms of oxidant stress and the development of disease in specific tissues lacking MnSOD. To circumvent this problem, a number of investigators have employed the Cre/loxP system to precisely knockout MnSOD in individual tissues. Recent Advances: Multiple tissue and organ-specific Creexpressing mice have been generated, which greatly enhance the specificity of MnSOD knockout in tissues and organ systems that were once difficult, if not impossible to study. Critical Issues: Evaluating the contribution of MnSOD deficiency to oxidant-mediated mitochondrial damage requires careful consideration of the promoter system used for creating the tissue-specific knockout animal, in addition to the collection and interpretation of multiple indices of oxidative stress and damage. Future Directions: Expanded use of well-characterized tissuespecific promoter elements and inducible systems to drive the Cre/loxP recombinational events will lead to a spectrum of MnSOD tissue knockout models, and a clearer understanding of the role of MnSOD in preventing mitochondrial dysfunction in human disease.

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“…Morris water maze, a classical test to assess physical fatigue, suggested that learning and memory efficiency was impaired in the model of POFS [13]. Furthermore, reactive oxygen species were associated with different types of fatigue [25][26][27][28][29]. Surgical trauma caused major oxidative stress [30,31], e.g.…”

Section: Nutritional State and Serum Levels Of Sod And Mdamentioning

confidence: 99%

Establishment and assessments of a new model for the postoperative fatigue syndrome by major small intestinal resection in rats

Zhang¹,

Chen²,

Dong³

et al. 2011

Scandinavian Journal of Gastroenterology

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. (2011). Establishment and assessments of a new model for the postoperative fatigue syndrome by major small intestinal resection in rats. Scandinavian Journal of Gastroenterology, 46(11), 1302-1309. DOI: 10.3109/00365521.2011 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal Morris water maze test were used to evaluate the degree of POFS. Serum albumin, transferrin, prealbumin and fibronectin were measured to assess the nutritional status, and superoxide dismutase (SOD) and malondealdehyde (MDA) were also measured. Results:As compared to the other 3 groups, the 70% small intestinal resection group showed the worst general state of health, decreased strength of the tail suspension test and the score of Morris water maze test (P<0.05) after operation.All rats suffering small intestinal resection demonstrated a certain degree of malnutrition and behaviour of depression, and the 70% resection group had the lowest levels of transferrin, prealbumin and fibronectin as compared to the other 3 groups (P<0.05), as well as decreased SOD and increased MDA in serum (P<0.05). Conclusions:Resection of 70% of the small intestine resulted in typical characteristics of POFS. As this procedure is simple, stable and easily reproducible, it may serve as a model for research on POFS.

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Oxidative stress in skeletal muscle causes severe disturbance of exercise activity without muscle atrophy

Cited by 110 publications

References 43 publications

Anti-fatigue Effect of Ginsenoside Rb1 on Postoperative Fatigue Syndrome Induced by Major Small Intestinal Resection in Rat

Anti-fatigue Effect of Ginsenoside Rb1 on Postoperative Fatigue Syndrome Induced by Major Small Intestinal Resection in Rat

The Use of the Cre/loxPSystem to Study Oxidative Stress in Tissue-Specific Manganese Superoxide Dismutase Knockout Models

Establishment and assessments of a new model for the postoperative fatigue syndrome by major small intestinal resection in rats

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