Oxidative stress in nonallergic nasal polyps associated with bronchial hyperresponsiveness<sup>1</sup>

Cheng, Yuan; Tsai, Ming-Hsui; Lin, Chia Der; Hwang, Guang-Yuh; Hang, Liang‐Wen; Tseng, Guan Chin; Shen, Pao‐Sheng; Chang, Wen Shin

doi:10.1111/j.1398-9995.2006.01228.x

Cited by 27 publications

(28 citation statements)

References 30 publications

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“…Dagli et al [17] investigated the role of free radicals and antioxidants in nasal polyps and suggested that the levels of antioxidants were decreased and the levels of oxidants were increased. Cheng et al [18] demonstrated that the mean level of tissue chemiluminescence in nasal polyps was significantly higher than control specimens, and the expression of superoxide dismutase 1 and 3 was higher in nasal polyp tissues. However, these studies focused on mucosal damage by ROS, while we focused on the role of ROS in signal mediators in TGF-β1-induced NPDFs.…”

Section: Discussionmentioning

confidence: 99%

Role of Caffeic Acid on Collagen Production in Nasal Polyp-Derived Fibroblasts

Chung

Park

Hong

et al. 2014

Clin Exp Otorhinolaryngol

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ObjectivesCaffeic acids are known to have anti-oxidant, anti-inflammatory, immunomodulatory, and tissue reparative effects. The purposes of this study were to determine the effect of caffeic acid on transforming growth factor (TGF) β1-induced myofibroblast differentiation and collagen production, and to determine whether caffeic acid is involved in the antioxidant effect in nasal polyp-derived fibroblasts (NPDFs).MethodsNPDFs were pretreated with caffeic acid (1-10 µM) for 2 hours and stimulated with TGF-β1 (5 ng/mL) for 24 hours. The expression of α-smooth muscle actin (SMA), collagen types I and III, and Nox4 mRNA was determined by a reverse transcription-polymerase chain reaction, and the expression of α-SMA protein was determined by actin ned by immunofluorescence microscopy. The amount of total soluble collagen production was analyzed by the Sircol collagen dye-binding assay. The reactive oxygen species (ROS) generated by NPDFs were determined using 2',7'-dichlorfluorescein-diacetate. siNox4 was used to determine the effect of Nox4.ResultsThe expression of α-SMA and production of collagen were significantly increased following TGF-β1 treatment. In contrast, the level of expression of α-SMA and the level of production of collagen were decreased by pretreatment with caffeic acid. The activation of Nox4 and the subsequent production of ROS were also reduced by pretreatment with caffeic acid. The expression of α-SMA was prevented by inhibition of ROS generation with siNox4.ConclusionCaffeic acid may inhibit TGF-β1-induced differentiation of fibroblasts into myofibroblasts and collagen production by regulating ROS.

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Section: Discussionmentioning

confidence: 99%

Role of Caffeic Acid on Collagen Production in Nasal Polyp-Derived Fibroblasts

Chung

Park

Hong

et al. 2014

Clin Exp Otorhinolaryngol

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“…Second, obesity characterised by increased systemic inflammation has been associated with increased progression of BHR and asthma 31. Third, Cheng et al 50 found a gradient of free radical concentrations in nasal polyps, an inflammatory chronic disease frequently associated with BHR and asthma. Concentrations were lowest in cases with nasal polyps without BHR and asthma, intermediate in those with concurrent silent BHR and highest in cases of nasal polyps with concurrent BHR and asthma.…”

Section: Discussionmentioning

confidence: 99%

Glutathione S-transferase genotype increases risk of progression from bronchial hyperresponsiveness to asthma in adults

Imboden

Rochat

Brutsche³

et al. 2008

Thorax

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Background: Bronchial hyperresponsiveness (BHR) and variation in glutathione S-transferase (GST) genes have been associated with asthma risk. The relationship of these two risk factors with adult onset asthma in the general population was investigated. Methods: GSTP1 Ile105Val single nucleotide polymorphism and GSTM1 and GSTT1 gene deletion polymorphisms were genotyped in the populationrepresentative SAPALDIA cohort. BHR was assessed at baseline by methacholine challenge and defined as a fall of >20% in forced expiratory volume in 1 s. Independent effects of GST polymorphisms and BHR on new onset of asthma after 11 years of follow-up were estimated by multiple logistic regression analysis, adjusting for relevant baseline measures. Effect modification was assessed by including interaction terms in the model. Results: Among 4426 asthma-free participants at baseline, 14% had BHR. At follow-up, 3.3% reported new onset of physician-diagnosed asthma. BHR (p,0.001) and GSTP1 Ile105Val genotype (p = 0.005) were independently associated with incident asthma, but no association was seen for GSTT1 and GSTM1 gene deletion polymorphisms. Among subjects free of respiratory symptoms at baseline, the effect of BHR on the risk of physician-diagnosed asthma at follow-up was restricted to GSTP1 105 Ile/Ile carriers (OR 4.57, 95% CI 2.43 to 8.57 vs 1.40, 95% CI 0.58 to 3.39; p for interaction = 0.023). Conclusions: If confirmed by independent studies, our results suggest that GSTP1 Ile105Val genotype strongly determines the progression of BHR to physician-diagnosed asthma in the general population.

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“…High levels of malondialdehyde, one of the metabolites of free radical-mediated lipid peroxidation, were observed in nasal polyps [8,9]. Moreover, free radical levels in nasal polyps were associated with nasal polyp severity [10,11]. The overproduction of free radicals and decrements in the antioxidant system can both cause tissue injury.…”

Section: Introductionmentioning

confidence: 99%

Reduction in Superoxide Dismutase Expression in the Epithelial Mucosa of Eosinophilic Chronic Rhinosinusitis with Nasal Polyps

Ono

Kusunoki²,

Miwa³

et al. 2013

Int Arch Allergy Immunol

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Background: Eosinophils generate large amounts of oxidant species. The eosinophil-dominant type of chronic rhinosinusitis (CRS) with nasal polyps is related to more extensive disease and a decreased likelihood of surgical success. Superoxide dismutase (SOD) is the first-line and only antioxidant enzyme that converts superoxide to hydrogen peroxide. Methods: The patients with CRS with nasal polyps were divided into eosinophilic and noneosinophilic groups. The expression of three isoforms of SOD, intracellular copper-zinc SOD (CuZnSOD), mitochondrial manganese SOD (MnSOD) and extracellular SOD (ECSOD), were examined by enzyme activity assay, immunohistochemistry and quantitative real-time RT-PCR sampled by laser capture microdissection. Results: SOD activity in the eosinophilic and noneosinophilic groups was significantly reduced compared to that of the control groups. Immunostaining of both CuZnSOD and MnSOD in the eosinophilic group was significantly decreased compared with that in the noneosinophilic and control groups. CuZnSOD mRNA of the eosinophilic group was significantly decreased compared with that of the control group, whereas MnSOD mRNA in the eosinophilic group was significantly decreased compared with that in the noneosinophilic and control groups. Neither immunoreactivity nor mRNA of ECSOD was different among the three groups. The degree of epithelial damage and disease severity were inversely correlated with CuZnSOD and MnSOD immunoreactivity. Conclusions: The reduction in SOD activity and the downregulation of the SOD message are suggested to be related to eosinophil recruitment and epithelial damage of CRS with nasal polyps.

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Oxidative stress in nonallergic nasal polyps associated with bronchial hyperresponsiveness¹

Cited by 27 publications

References 30 publications

Role of Caffeic Acid on Collagen Production in Nasal Polyp-Derived Fibroblasts

Role of Caffeic Acid on Collagen Production in Nasal Polyp-Derived Fibroblasts

Glutathione S-transferase genotype increases risk of progression from bronchial hyperresponsiveness to asthma in adults

Reduction in Superoxide Dismutase Expression in the Epithelial Mucosa of Eosinophilic Chronic Rhinosinusitis with Nasal Polyps

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Oxidative stress in nonallergic nasal polyps associated with bronchial hyperresponsiveness1

Cited by 27 publications

References 30 publications

Role of Caffeic Acid on Collagen Production in Nasal Polyp-Derived Fibroblasts

Role of Caffeic Acid on Collagen Production in Nasal Polyp-Derived Fibroblasts

Glutathione S-transferase genotype increases risk of progression from bronchial hyperresponsiveness to asthma in adults

Reduction in Superoxide Dismutase Expression in the Epithelial Mucosa of Eosinophilic Chronic Rhinosinusitis with Nasal Polyps

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Oxidative stress in nonallergic nasal polyps associated with bronchial hyperresponsiveness¹