Oxidative stress in mice infected with Angiostrongylus cantonensis coincides with enhanced glutathione-dependent enzymes activity

Chung, Li-Yu; Chen, Chun-Hsiang; Wang, Lian-Chen; Chang, Shun‐Jen; Yen, Chuan-Min

doi:10.1016/j.exppara.2010.04.017

Cited by 17 publications

(9 citation statements)

References 39 publications

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“…In A. cantonensis infection, reactive stress and injury are elevated in the parenchyma, meninges, and cerebrospinal fluid of the mouse brain [ 37 , 38 ]. Moreover, this nematode induces blood-brain barrier dysfunction and immune responses in the host brain [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Stimulation of IL-1β and IL-6 through NF-κB and sonic hedgehog-dependent pathways in mouse astrocytes by excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis

Chen

Wang

2017

Parasites Vectors

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Background: Angiostrongylus cantonensis is an important causative agent of eosinophilic meningitis and eosinophilic meningoencephalitis in humans. Previous studies have shown that the Sonic hedgehog (Shh) signaling pathway may reduce cell apoptosis by inhibiting oxidative stress in A. cantonensis infection. In this study, we investigated the relationship between cytokine secretion and Shh pathway activation after treatment with excretory/secretory products (ESP) of fifth-stage larval A. cantonensis (L5).

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Section: Discussionmentioning

confidence: 99%

Stimulation of IL-1β and IL-6 through NF-κB and sonic hedgehog-dependent pathways in mouse astrocytes by excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis

Chen

Wang

2017

Parasites Vectors

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“…These findings are consistent with those observed in A . cantonensis -infected mice with meningoencephalitis [ 31 ]. Moreover, the larvae have been reported to cause severe eosinophilic meningoencephalitis in infected BALB/c mice.…”

Section: Discussionmentioning

confidence: 99%

“…In experimentally infected mice, blood-brain barrier (BBB) dysfunction and CSF eosinophilia always occur [ 30 ]. Chung et al [ 31 , 32 ] reported that A . cantonensis infection may cause elevation of ROS and antioxidants in the CSF of infected mice.…”

Section: Introductionmentioning

confidence: 99%

Activation of Sonic Hedgehog Leads to Survival Enhancement of Astrocytes via the GRP78-Dependent Pathway in Mice Infected withAngiostrongylus cantonensis

Chen

Cheng

Wang

2015

BioMed Research International

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Angiostrongylus cantonensis infection may cause elevation of ROS and antioxidants in the CSF of infected mice. Astrocytes may protect the surrounding neurons from oxidative stress-induced cell death by secreting Sonic hedgehog (Shh) via the PI3-K/AKT/Bcl-2 pathway. This study was conducted to determine the role of the Shh signaling pathway in A. cantonensis-infected BABL/c mice by coculturing astrocytes with living fifth-stage larvae or soluble antigens. The Shh pathway was activated with corresponding increases in the level of the Shh. Glial fibrillary acidic protein (GFAP) and Shh were increased in astrocyte cocultured with living fifth-stage larvae or soluble antigens. The survival of astrocytes pretreated with Shh was significantly elevated in cocultures with the antigens but reduced by its inhibitor cyclopamine. The expression of GRP78 and Bcl-2 was significantly higher in astrocytes pretreated with recombinant Shh. These findings suggest that the expression of Shh may inhibit cell death by activating Bcl-2 through a GRP78-dependent pathway.

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“…It has been demonstrated that the expression of ROS-related enzymes such as glutathione reductase, glutathione peroxidase, and glutathione S-transferase (GST) significantly increases in the CSF of mice infected with A . cantonensis (Chung et al 2010). …”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of the fifth-stage larvae of Angiostrongylus cantonensis by next-generation sequencing

et al. 2013

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Angiostrongylus cantonensis is an important zoonotic nematode. It is the causative agent of eosinophilic meningitis and eosinophilic meningoencephalitis in humans. However, information of this parasite at the genomic level is very limited. In the present study, the transcriptomic profiles of the fifth-stage larvae (L5) of A. cantonensis were investigated by next-generation sequencing (NGS). In the NGS database established from the larvae isolated from the brain of Sprague–Dawley rats, 31,487 unique genes with a mean length of 617 nucleotides were assembled. These genes were found to have a 46.08 % significant similarity to Caenorhabditis elegans by BLASTx. They were then compared with the expressed sequence tags of 18 other nematodes, and significant matches of 36.09–59.12 % were found. Among these genes, 3,338 were found to participate in 124 Kyoto Encyclopedia of Genes and Genomes pathways. These pathways included 1,514 metabolisms, 846 genetic information processing, 358 environmental information processing, 264 cellular processes, and 91 organismal systems. Analysis of 30,816 sequences with the gene ontology database indicated that their annotations included 5,656 biological processes (3,364 cellular processes, 3,061 developmental processes, and 3,191 multicellular organismal processes), 7,218 molecular functions (4,597 binding and 3,084 catalytic activities), and 4,719 cellular components (4,459 cell parts and 4,466 cells). Moreover, stress-related genes (112 heat stress and 33 oxidation stress) and genes for proteases (159) were not uncommon. This study is the first NGS-based study to set up a transcriptomic database of A. cantonensis L5. The results provide new insights into the survival, development, and host–parasite interactions of this blood-feeding nematode.Electronic supplementary materialThe online version of this article (doi:10.1007/s00436-013-3495-z) contains supplementary material, which is available to authorized users.

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Oxidative stress in mice infected with Angiostrongylus cantonensis coincides with enhanced glutathione-dependent enzymes activity

Cited by 17 publications

References 39 publications

Stimulation of IL-1β and IL-6 through NF-κB and sonic hedgehog-dependent pathways in mouse astrocytes by excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis

Stimulation of IL-1β and IL-6 through NF-κB and sonic hedgehog-dependent pathways in mouse astrocytes by excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis

Activation of Sonic Hedgehog Leads to Survival Enhancement of Astrocytes via the GRP78-Dependent Pathway in Mice Infected withAngiostrongylus cantonensis

Transcriptome profiling of the fifth-stage larvae of Angiostrongylus cantonensis by next-generation sequencing

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